Improved Long-Term Stability of N-Acetylneuraminic Acid and N-Glycolylneuraminic Acid Peak Area Responses Using Waveform A, a Quadruple Potential Waveform

Technical Note 41 Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography INTRODUCTION EQUIPMENT Sialic acids comprise a large family of N- and O-substituted neuraminic acids. The amino group of neuraminic acid is linked to either an N-acetyl or N-glycolyl group,…

Application Update 180 Direct Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD INTRODUCTION Sialic acids are critical in determining glycoprotein bioavailability, function, stability, and metabolism.1 Although over 50 natural sialic acids have been identified,2 two forms are commonly determined…

Application Update: 181 Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD Deanna Hurum and Jeff Rohrer Introduction • Neuraminic Acids • Anion Exchange • Pulsed Amperometric Detection • CarboPac PA20 Fast Sialic Acid Column • High-Throughput Analysis Glycoprotein sialylation…

Sialic Acid Determination in Glycoproteins: Comparison of Two Liquid Chromatography Methods Deanne Hurum and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Introduction Experimental Sialic acids are critical in determining glycoprotein bioavailability, function, stability, and eventual catabolism. Although over 50…