Direct Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD

Application Update: 181 Rapid Screening of Sialic Acids in Glycoproteins by HPAE-PAD Deanna Hurum and Jeff Rohrer Introduction • Neuraminic Acids • Anion Exchange • Pulsed Amperometric Detection • CarboPac PA20 Fast Sialic Acid Column • High-Throughput Analysis Glycoprotein sialylation…

APPLICATION NOTE 74121 Determination of glycoprotein sialic acid composition using HPAE-PAD in dual eluent generation cartridge mode Authors: Jingli Hu and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Dionex CarboPac PA20-1 mm column, Dionex ICS-6000 HPIC system, electrochemical detection,…

Sialic Acid Determination in Glycoproteins: Comparison of Two Liquid Chromatography Methods Deanne Hurum and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Introduction Experimental Sialic acids are critical in determining glycoprotein bioavailability, function, stability, and eventual catabolism. Although over 50…

Table of Contents Introduction Applications Innovative Analytical Technologies Monosaccharide and Sialic Acid Determinations in Biosimilars Using HPAE-PAD Introduction Table of Contents Introduction Applications Innovative Analytical Technologies A large number of innovator biotherapeutics are currently losing patent protection, resulting…