Analysis of Exoglycosidase Digestions of N-Linked Oligosaccharides Using HPAE-PAD

Deanna Hurum, Lipika Basumallick, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Prostate-Specific Antigen (PSA), Transferrin, N-Glycans, O-Glycans, Dionex CarboPac Column Introduction Changes in protein glycosylation are frequently studied in cancer research to identify potential biomarkers. Factors…

Application Note 105 Glycosylation Analysis of Human Serum Transferrin Glycoforms Using Pellicular Anion-Exchange Chromatography INTRODUCTION Glycoforms and Protein Sialylation Glycoproteins are proteins with a carbohydrate attached to the polypeptide backbones through one or more glycosylation sites. Oligosaccharides can be linked…

APPLICATION NOTE 72914 HPAE-PAD profiling of N-linked oligosaccharides from glycoproteins using dual eluent generation cartridges Authors Beibei Huang and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Goal Demonstrate Dual EGC capability and performance for profiling N-linked oligosaccharides released from…

TECHNICAL NOTE 72264 HPAE-PAD N-linked oligosaccharide profiling of IgG Authors Sachin Patil, Deanna Hurum, Lipika Basumallick, and Jeff Rohrer Thermo Fisher Scientific, Sunnyvale, CA Goal To develop an HPAE-PAD method to profile the major neutral and charged asparagine-linked (N-linked) oligosaccharides…