Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride (PVDF) Membranes

APPLICATION UPDATE 176 Preparation of peptide N-Glycosidase F digests for HPAE-PAD analysis Authors Introduction Pranathi Perati and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Glycosylation is one of the most important post-translational modifications in eukaryotic cell proteins. Glycoproteins are involved…

Technical Note 42 Glycoprotein Oligosaccharide Analysis Using HighPerformance Anion-Exchange Chromatography INTRODUCTION The development of recombinant-derived glycoproteins for therapeutic use has led to an increasing demand for methods to characterize their carbohydrate structures. High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD)…

Application Note 215 Separation of Asparagine-Linked (N-Linked) Oligosaccharides from Human Polyclonal IgG Using the CarboPac PA200 Column Introduction High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a widely used technique for determining an extensive set of carbohydrates including, but…

TECHNICAL NOTE 70671 Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) Author: Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: Technical Note 20, monosaccharide, alditol, glycan, oligosaccharide, sialic acid, sugar Goal Update Dionex Technical Note 20 to…