Improved Middle-Down Characterization of Antibodies Using Multiple Ion Activation Techniques and Ion-Ion Proton Transfer Reactions on a Modified Orbitrap Mass Spectrometer

Technical note | 003318 Biopharma In-depth characterization of monoclonal antibodies Intact mass analysis and middle-down mass spectrometry approaches on an Orbitrap Ascend BioPharma Tribrid mass spectrometer Authors Goal Jingjing Huang, Christopher Mullen, To assess the performance of the Thermo Scientific™…

APPLICATION NOTE 74160 Proton transfer charge reduction (PTCR) improves spectral matching and sequence coverage in middle-down analysis of monoclonal antibodies Luca Fornelli,1 Ryan N. Oates,1 Kristina Srzentić,2 Christopher Mullen,3 John E. P. Syka,3 and Romain Huguet3 University of Oklahoma, Norman,…

Poster Note 64778 Top-down Characterization of Monoclonal Antibody on an Orbitrap Fusion Lumos™ Tribrid Mass Spectrometer Seema Sharma, Stephane Houel, Christopher Mullen, Chad Weisbrod, Romain Huguet, John Syka, Dave Horn, Jonathan Josephs, Jae Schwartz, Vlad Zabrouskov Thermo Fisher Scientific, San…

WHITE PAPER 65971 Grant application resource Go beyond today’s discovery with the Orbitrap Eclipse Tribrid mass spectrometer Keywords: Orbitrap Eclipse Tribrid Mass Spectrometer, FAIMS Pro Interface, Mass Spectrometry, Quantitative Proteomics, TMT and TMTpro Multiplexing, Single-cell Sensitivity, Top-down Proteomics, Native Intact…