Multi-Attribute Monitoring of Antibody Variants through Hydrophobic and Cation- Exchange Chromatography Coupled with Native Mass Spectrometry

Poster Reprint ASMS 2023 Poster number MP 626 Multi-attribute monitoring of aggregates and charge variants of monoclonal antibody through native 2DSEC-MS-WCX-MS Sunil Kumar1, Tushar Sharad Savane1, Vadiraja Bhat2, Mike Knierman3, Anurag Singh Rathore1 1 Department of Chemical Engineering, Indian Institute…

Poster Reprint ASMS 2023 Poster number MP 630 Rapid analysis of titer, aggregate and intact mass of antibody therapeutics using multi-dimensional liquid chromatography coupled with native mass spectroscopy Tushar Sharad Savane1, Sunil Kumar1, Vadiraja Bhat2, Anurag Singh Rathore1 1 Department…

Application Note Biopharma/Pharma Characterization of Antibody‑Drug Conjugates Using 2D-LC and Native MS Authors David L. Wong and Sarah M. Stow Agilent Technologies, Inc. Abstract Antibody drug conjugates (ADCs), which comprise a monoclonal antibody (mAb) conjugated to a small molecule drug…

Application Note Biopharma/Pharma Determination of Multiple Attributes of Monoclonal Antibodies Simultaneous and parallel multi-attribute analysis using 3D-LC/MS with 2D multimethod option mAb SEC Size variants Protein A CEX LC/MS mAb Titer Charge variants Mol wt (MW) AA sequence PTM Cell…