Rapid Profiling of Proteomes and Sub-Proteomes on the Orbitrap Fusion Tribrid Mass Spectrometer

Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…

evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…

Xiaoyue Jiang1, David Horn1, Michael Blank1, Devin Drew1, Bernard Delanghe2, Rosa Viner1, Andreas FR Huhmer1 1 Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Bremen, Germany Key Words Protein coverage, post-translational modification (PTM), Proteome Discoverer, SEQUEST, Byonic, Mascot,…

Large Scale Targeted Protein Quantification Using WiSIM-DIA Workflow on a Orbitrap Fusion Tribrid Mass Spectrometer Reiko Kiyonami,1 Bhavin Patel, 3 Michael Senko,1 Vlad Zabrouskov,1 Jarrett Egertson, 2 Sonia Ting, 2 Michael MacCoss, 2 John Rogers, 3 Andreas FR Hühmer1 1…