Top-down Analysis of Intact Antibodies under Denatured and Native Conditions on the Omnitrap Platform Coupled to an Orbitrap Mass Spectrometer

Novel ion activation workflows in the hybrid Orbitrap-Omnitrap platform empower top-down and bottom-up proteomics Dimitris Papanastasiou1; Athanasios Smyrnakis1; Mariangela Kosmopoulou1; Kyle L. Fort2; Maria Reinhardt-Szyba2; Susanna Lundström3; Jan Commandeur4; Julia Chamot-Rooke5; Tingting Fu5; Yury Tsybin6; Konstantin Nagornov6; Anton Kozhinov6; David…

AS MS 2 0 2 5 – M P 7 1 3 n Offline tandem MS workflows on the timsOmni platform for deep sequencing of intact proteins and mAbs Athanasios Smyrnakis1, Mariangela Kosmopoulou1, Anastasios Grigoriadis1, Florian Busch2, Stuart Pengelley3, Eduardo…

Top-down sequencing of bispecific antibodies by electron capture dissociation on a timsOmni platform Iuliia Stroganova1,2, Simon Ollivier1,2, Jean-François Greisch1,5, Athanasios Smyrnakis3, Mariangela Kosmopoulou3, Detlev Suckau4, Dimitris Papanastasiou3 and Albert J.R. Heck1,2 1Biomolecular Mass Spectrometry & Proteomics, NL, 2Netherlands Proteomics Center,…

Applying cDDA-eXd on a timsOmni to the analysis of the Fab regions of antibodies originating from human serum Simon Ollivier1,2, Manuel Bauer5, Dina Schuster1,2, Athanasios Smyrnakis3, Jan Fiala1,2, Stuart Pengelley4, Oliver Raether4, Mariangela Kosmopoulou3, Detlev Suckau4, Jean-François Greisch1,5, Dimitris Papanastasiou3…