Faster protein digestion without loss of fidelity: rapid desalting, optimized buffers and heat-stabilized immobilized trypsin

Peptide Mapping for Biotherapeutics Strategies for Simplifying Protein Digestion A sponsored publication from Peptide Mapping Doesn’t Need to Be Complex Introducing PeptideWorks™ Tryptic Protein Digestion Kits PeptideWorks Tryptic Protein Digestion Kits uniquely deliver automatable, high efficiency, reproducible peptide maps in…

Application Note Quick and Robust Sample Preparation for Tryptic Peptide Mapping With the PeptideWorks™ Kit Using Simple, Automatable Workflows Caitlin M. Hanna, Jonathan P. Danaceau, Stephan M. Koza, Steve Shiner, Mary Trudeau Waters Corporation Abstract Here, we present an automatable…

IMPROVED BIOPHARMACEUTICAL PEPTIDE MAPPING WORKFLOWS USING A NOVEL AUTOLYSIS-RESISTANT TRYPSIN ENZYME Samantha Ippoliti1, Nick Zampa1, Ying Qing Yu1, Matthew A Lauber1, Leslie Napoletano1, Scott Berger1 Waters Corporation, Milford, MA METHODS Peptide mapping provides a wealth of information about a protein…

Application Note Automated High-Throughput LC-MS Focused Peptide Mapping of Monoclonal Antibodies in Microbioreactor Samples Hua Yang, Stephan M. Koza, Ying Qing Yu Waters Corporation Abstract To facilitate cell line selection and bioprocess optimization, an automated high-throughput (HT) sample preparation method…