Subunit Level Top-down ECD Characterization of Biotherapeutic Proteins: Improving Mass Spectral Clarity with Fragment Ion Level Ion Mobility

EXPANDING NATIVE MASS SPECTROMETRY CAPABILITIES FOR SOLUBLE AND MEMBRANE PROTEINS USING A QUADRUPOLE-ION MOBILITY-TIME-OF-FLIGHT MASS SPECTROMETRY SYSTEM Brad Williams1, Dale A. Cooper-Shepherd2, Mario Hensen3, Kleitos Sokratos3, Jonathan T.S. Hopper3, and James I. Langridge2 1. Waters Corporation, 34 Maple Drive, Milford,…

In-Depth Versatile Characterization of Aspartic Acid Isomers via a Cyclic Ion Mobility Mass Spectrometry (cIMS) Samantha Ippoliti1; Brad J. Williams1, Dale Cooper-Shepherd2, Ying Qing Yu1, Weibin Chen1, Barbara Sullivan1, James I. Langridge2 1Waters Corporation, Milford, MA, USA; 2Waters Corporation, Wilmslow,…

Application Note Electron Capture Dissociation in Combination With High Performance Cyclic Ion Mobility Separation for Detailed Protein Studies Dale Cooper-Shepherd Waters Corporation This is an Application Brief and does not contain a detailed Experimental section. Abstract Electron capture dissociation (ECD)…

Increasing sensitivity in peptide-level studies; Cyclic ECD product ions Dale A. Cooper-Shepherd1, Isabella A. Jones1, Emma Marsden-Edwards1, Ramin Rabbani2 1Waters Corporation, Wilmslow, Cheshire, UK; 2Waters Corporation, Milford, MA, USA IMS-enabled Wideband Enhancement of CID and INTRODUCTION OPTIMIZATION OF CYCLIC IMS…