Fully automated glycoform profiling and sequence validation of the NIST reference antibody

Protein Clipping Variant Analysis of Vedolizumab using dedicated workflows in BioPharma Compass Proteolytic degradation of biopharmaceuticals during their life cycle can pose health risks and require dedicated analysis. Abstract In this work we used enzymatically introduced antibody truncation variants as…

Extensive LC-Top-Down MS Sequence Confirmation of the NISTmAb Reference Material 8671 Characterization of therapeutic antibodies aims on primary sequence validation, localization of modifications such as glycan profiles and the determination of disulfide bond status of the target molecule. Introduction Typically,…

Routine analysis of drug to antibody ratio and drug distribution with maXis II Antibody drug conjugates (ADC) are small molecule conjugated mAbs. These are rapidly emerging complex biological molecules in the biopharma space. Abstract Characterization of ADC’s includes intact mass…

Automated High-Throughput Clone Screening of Therapeutic Antibodies: From Automated IdeS Digestion to Intact Fc/2-Glycosylation Analysis by MALDI-TOF The glycosylation status of the Fc/2 region is a critical quality attribute (CQA) of therapeutic antibodies and is typically tested in early stages…