Skyline Support for Proteome-wide Data Analysis of Bruker timsTOF dia-PASEF Acquisition

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…

prm-PASEF®: enabling high-throughput, high sensitivity targeted proteomics The prm-PASEF acquisition method has been developed to translate the advantages of the parallel accumulation serial fragmentation (PASEF) acquisition strategy to the targeted proteomics field. Abstract In comparison with standard selected and parallel…

High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and…