New Paradigm for HPLC Method Development

VOL 18.6 TheReporter www.sigma-aldrich.com/TheReporter (continued on page 3) LC issue Conditions for A, B & C differ only in column length, flow and gradient. Panel A. Column: Discovery C18, 15cm x 4.6mm ID, 5µ Cat. No.: 504955 Sample: Gradient RP…

595 North Harrison Road Bellefonte, PA 16823-0048 USA Telephone 800-247-6628 • 814-359-3441 Fax 800-447-3044 • 814-359-3044 email: supelco@s ial.com sigma-aldrich.com/supelco Bulletin 937 Use Discovery to Reduce HPLC Method Development Time Following the Discovery method development protocol and using the Column…

VOL 18.3 TheReporter www.sigma-aldrich.com/TheReporter Optimizing HPLC Separations: Samples with Widely Differing Polarities When confronted with a sample containing widely varying polarities, the LC chromatographer will typically rely on gradient elution. However, if gradient elution is not an option, isocratic elution…

HPLC Column Comparison Screening Study for Reversed Phase Columns Carmen T. Santasania, David S. Bell, and Tracy L. Ascah-Ross Supelco, 595 North Harrison Road, Bellefonte, PA 16823, USA T403157 GIB Introduction The selection of the proper HPLC column for any…