Improved Peak Recovery and Peak Shape of Oligonucleotides Using Waters PREMIER Columns

Application Note Mitigation of Non-Specific Adsorption During Column Conditioning for an Oligonucleotide HILIC Analysis Tony Reinhold, Martin Gilar, Andrew Steere, Paula Hong Waters Corporation Abstract Oligonucleotides, the short strands of nucleic acids that are pivotal in genetic research and therapeutic…

Application Note Deploying the Alliance™ iS Bio HPLC System as a Modern HPLC for Biopharmaceutical Analysis in QC Environments Xiangsha Du, Robert E. Birdsall, Pawel Bigos, Duanduan Han, Karen Nyholm Waters Corporation Abstract The Alliance iS Bio HPLC System with…

Application Note Improved Chromatographic Analysis of Oligonucleotides with ACQUITY PREMIER Oligonucleotide BEH C18 Columns Martin Gilar, Zhimin Li, Jason MacLean Waters Corporation Abstract This application note illustrates sources of oligonucleotide loss due to adsorption on metallic column hardware (frits). Solutions,…

Application Note HILIC as an Alternative Separation Mode for Intact Mass Confirmation of Oligonucleotides on the BioAccord System Catalin E. Doneanu, Ying Qing Yu, Joseph Fredette, William J. Warren, Weibin Chen Waters Corporation Abstract HILIC chromatography provides a clean, cost-effective…