Successful Transfer of Size-Exclusion Separations Between HPLC and UPLC

Advanced HPLC Size-Exclusion Chromatography for the Analysis of Macromolecular Proteins Using 3.5 µm Ethylene Bridged Hybrid (BEH) Particles Stephan Koza, Susan Serpa, Hua Yang, Edouard Bouvier, and Kenneth J. Fountain Waters Corporation, Milford, MA, USA A P P L I…

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

XBridge Protein BEH SEC Columns for HPLC-based Separations Waters XBridge ® Protein BEH SEC, 200Å and 450Å, 3.5 µm Columns were developed for use on HPLC instrumentation and to complement our existing line of smaller particle sized, UPLC ® -based…

Application Note Ultimate Resolution HPLC-Based Size Exclusion Chromatography for the Analysis of Small Proteins and Peptides Using 3.5 μ m Ethylene Bridged Hybrid (BEH) Particles Susan Serpa, Hua Yang, Edouard Bouvier, Stephan M. Koza Waters Corporation Abstract The introduction of…