Advanced HPLC Size-Exclusion Chromatography for the Analysis of Macromolecular Proteins Using 3.5 μm Ethylene Bridged Hybrid (BEH) Particles

XBridge Protein BEH SEC Columns for HPLC-based Separations Waters XBridge ® Protein BEH SEC, 200Å and 450Å, 3.5 µm Columns were developed for use on HPLC instrumentation and to complement our existing line of smaller particle sized, UPLC ® -based…

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

Successful Transfer of Size-Exclusion Separations Between HPLC and UPLC Stephan Koza, Susan Serpa, Edouard Bouvier, and Kenneth J. Fountain Waters Corporation, Milford, MA, USA A P P L I C AT I O N B E N E F I…

The Analysis of Multimeric Monoclonal Antibody Aggregates by Size-Exclusion UPLC Stephan Koza, Matthew Lauber, and Kenneth J. Fountain Waters Corporation, Milford, MA, USA A P P L I C AT I O N B E N E F I T…