GC Analysis of Derivatized Amino Acids

Anila I Khan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Key Words Derivatization reagent, silylation, N-methyl-N–(trimethylsilyl) trifluoroacetamide (MSTFA), L-alanine, L-leucine, L-lysine, amino acid Abstract The derivatization of amino acids was achieved using the MSTFA silylation reagent. The TFA derivatized compounds were…

Thermo Scientific Reagents, Solvents and Accessories • Gas Chromatography • High Performance Liquid Chromatography • Mass Spectrometry Table of Contents Thermo Scientific Products for GC and HPLC Amino Acid Analysis 33-43 Derivatization Reagents for Specific Functional Groups 1-2 Derivatization Reagents…

Anila I Khan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Key Words Derivatization reagent, acylation, N-methyl-bis(trifluoroacetamide) (MBTFA) glucose, fructose, sugars, TR-1701 Abstract Sugars must be derivatized to a volatile form so as to be analyzed by GC. A commonly used derivatization…

Anila I Khan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Appli cat i on N ote 2 0 5 5 9 GC Analysis of Derivatized Chlorinated Acetic Acids Key Words Derivatization reagent, extractive alkylation, pentafluorobenzyl bromide (PFBBr), chloroacetic acid (CA), dichloroacetic…