The Impact of Adducts and Strategies to Control Them in IP-RPLC Based Oligonucleotide Analyses

[ APPLICATION NOTE ] Evaluation of Alternative Ion-pairing Reagents in the Analysis of Oligonucleotides with the ACQUITY QDa Detector Robert E. Birdsall and Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Method compatibility with alternative Electrospray…

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…

Extending the Lower Limits of Quantification of a Therapeutic Oligonucleotide Through Microflow LC-MS/MS Featuring SCIEX QTRAP® 6500+ LC-MS/MS System with OptiFlow™ Turbo V source and the SCIEX M5 MicroLC System Daniel Warren, Sean McCarthy, Lei Xiong SCIEX, Framingham, MA, USA…

Application Note BioPharma/Pharma Eliminate Metal Ions from Your Oligonucleotide LC/MS Analysis Avoid undesirable metal interactions with Agilent Ultra Inert HPLC column hardware and fully biocompatible LC instrumentation Authors Abstract Andrew Coffey, Andrea Angelo P. Tripodi, Anne Blackwell, and Jordy Hsiao…