Factors Influencing Diastereomer Separations in Oligonucleotide Analysis

Product spotlight | 000477 Oligonucleotide characterization Oligonucleotide characterization using μPAC columns in ion-pairing reversed-phase high performance liquid chromatography mode Abstract Single stranded oligonucleotides such as antisense oligonucleotides (ASOs) represent a Keywords broad-spectrum therapeutic platform. Ion-pairing reversed-phase high performance liquid Micro…

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…

Application Note Platform Ion Pairing RPLC Method for Oligonucleotides Using High Throughput 20 mm Length Columns Szabolcs Fekete, Mateusz Imiolek, Matthew A. Lauber Waters Corporation Abstract A novel generic (platform) method is proposed for the separation of oligonucleotide size variants…

An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations: From Analysis to Purification Melissa Goodlad, PhD Columns and Supplies Applications Engineer April 15, 2025 1 April 15, 2025 An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations DE-006001 Introduction to Ion-Paired Reverse-Phase (IP-RP) Separations…