<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 2nd November 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><h3><strong>1. Agilent Technologies: Automated Workflow for Peptide Mapping of Pembrolizumab on the Agilent 6545XT AdvanceBio LC/Q-TOF</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_peptide_mapping_pembrolizumab_5994_9456en_agilent_9e452e569c.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><div><div><div><div><div><div><div><div><div><div><div><div><div><p><span>Therapeutic monoclonal antibodies (mAbs) are structurally complex biopharmaceuticals that require detailed characterization to ensure safety, efficacy, and batch‑to‑batch consistency. Due to their heterogeneity, arising from post‑translational modifications (PTMs) such as glycosylation, oxidation, and deamidation, comprehensive analytical strategies are essential.&nbsp;</span></p><p><span>By digesting the therapeutic protein into peptides and analyzing them using LC/MS/MS, this workflow delivers comprehensive sequence-level characterization and a high‑resolution molecular fingerprint, enabling:</span></p><ul><li><span>Confirmation of primary amino acid sequence&nbsp;</span></li><li><span>Identification and localization of PTMs&nbsp;</span></li><li><span>Monitoring critical quality attributes (CQAs) throughout development and manufacturing&nbsp;</span></li></ul><p><span>The Agilent AssayMAP Bravo Protein Sample Prep platform automates mAb sample preparation workflows, including digestion and peptide cleanup, to improve reproducibility, reduce hands-on time, and increase throughput. Automated processing ensures consistent peptide mapping results and supports reliable characterization of CQAs in biotherapeutics.</span></p><h4><span><strong>Experimental</strong></span></h4><h5><span><strong>Instruments&nbsp;</strong></span></h5><ul><li><span>Agilent 1290 Infinity III bio high-speed pump (G7132A)&nbsp;</span></li><li><span>Agilent 1290 Infinity III bio multisampler (G7137A) with Agilent InfinityLab sample thermostat (G7167A-60201)&nbsp;</span></li><li><span>Agilent 1290 Infinity III multicolumn thermostat (G7116B)&nbsp;</span></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=6545XT"><span><strong>Agilent 6545XT AdvanceBio LC/Q-TOF</strong></span></a><span> with Agilent Dual Jet Stream ESI source</span></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=AssayMAP+Bravo"><span><strong>Agilent AssayMAP Bravo system</strong></span></a><span> (G5542A) – for reduced and nonreduced analysis&nbsp;</span></li></ul><h5><span><strong>Software&nbsp;</strong></span></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=MassHunter+data+acquisition"><span><strong>Agilent MassHunter data acquisition software</strong></span></a><span> for LC/TOF and LC/Q-TOF version 11.1&nbsp;</span></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=MassHunter+BioConfirm"><span><strong>Agilent MassHunter BioConfirm software</strong></span></a><span> version 12.1&nbsp;</span></li><li><span>Agilent VWorks version 13.1</span></li></ul><h4><span><strong>Conclusion&nbsp;</strong></span></h4><p><span>A comprehensive workflow has been demonstrated, encompassing automated sample preparation on an Agilent&nbsp;AssayMAP Bravo platform, LC separation using an Agilent 1290 Infinity III bio LC system, data acquisition with an Agilent 6545XT AdvanceBio LC/QTOF, and data analysis through Agilent MassHunter BioConfirm software. This approach enables amino acid sequence identification and confirmation, verification of the intended S228P mutation, terminal sequence analysis, as well as characterization of PTMs including the low-abundance oxidation form and disulfide linkages in pembrolizumab mAb.</span></p><p><span>The high-quality MS/MS spectra, combined with extensive coverage of low-abundance peptides, underscore the strong performance of the Agilent 6545XT AdvanceBio LC/QTOF for peptide mapping applications.</span></p></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div><h3><strong>2. Shimadzu: Quantitative Analysis of Selected Per- and Polyfluoroalkyl Substances (PFAS) in Cosmetic Products by LC-MS/MS</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_07_2608_en_89b912446a.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User benefits</strong></h5><ul><li>Accurate quantification of multiple PFAS in complex cosmetic matrices is possible using LC-MS/MS with internal-standard calibration.&nbsp;</li><li>QuEChERS extraction combined with SPE cleanup provides effective matrix removal, enabling reliable PFAS analysis in diverse cosmetic products.&nbsp;</li><li>The PFAS-targeted method can be expanded to include additional PFAS compounds as analytical requirements evolve.</li></ul><p>Per- and polyfluoroalkyl substances (PFAS) are a diverse group of synthetic chemicals that have been intentionally used in certain cosmetic products because of their unique physicochemical properties and functional characteristics, including water and oil resistance, conditioning, and film formation<sup>1</sup>. These properties can contribute to the performance and sensory characteristics of cosmetic products. However, concerns regarding the persistence and potential environmental and health impacts of certain PFAS have increased the need for reliable analytical methods to identify and quantify these compounds in cosmetic products<sup>2</sup>. This Application News presents a workflow for the quantitative analysis of selected PFAS in cosmetic products using <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=8060RX"><strong>Shimadzu’s LCMS-8060RX triple quadrupole mass spectrometer</strong></a> coupled with the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+LC-40"><strong>Nexera LC-40 ultra-high-performance liquid chromatography system</strong></a> (Fig. 1).</p><h4><strong>Conclusion</strong></h4><p>The developed workflow provides a simple and effective approach for the extraction and quantitative analysis of PFAS in cosmetic products. The QuEChERS-based extraction combined with SPE cleanup provides effective matrix reduction, although it does not eliminate matrix-related effects. The cleanup helps minimize matrix components that can contribute to ion suppression and contamination of the LC-MS/MS ion source. The use of PFAS-free materials throughout the analytical workflow further minimizes the risk of background contamination. Combined with the high sensitivity of the LCMS-8060 RX, the developed workflow demonstrated good analytical performance for the targeted PFAS compounds in cosmetic matrices. The developed sample preparation protocol can be extended to HRMS platforms such as the LCMS-9050 for non-targeted PFAS analysis, enabling the screening and identification of a broader range of PFAS beyond the targeted compounds evaluated in this study.</p><h3><strong>3. Thermo Fisher Scientific: Out-of-the-box workflow for PFAS quantitation using a triple quadrupole approach with the TSQ Altis Plus mass spectrometer</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/ab_003941_lsms_pfas_quantitation_tsq_altis_plus_ab003941_em_en_6e529f20ab.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>Application benefits&nbsp;</strong></h5><ul><li>Quantitation of a large panel of 61 PFAS compounds with performance and robustness proved with the applied analytical strategy.</li><li>Designed to comply with current European regulations (0.1 µg/L sum of 20 PFAS; 0.5 µg/L total PFAS), and with many local regulations.</li><li>The sensitivity of our method is compatible with major sample preparation techniques, including solid phase extraction (SPE). Limits of Quantification (LOQs) are achieved in solvent ranges from 50 ng/L for over 80% of targets to 500 ng/L for fewer than 5% of targets. Corresponding LOQs in matrices range from 0.1 ng/L to 1 ng/L with 500-fold concentration.</li><li>Challenging PFAS (FTOH, FTAB) on the same method with a single HPLC separation.</li></ul><p>Per- and polyfluoroalkyl substances (PFAS) are a large group of synthetic chemicals known for their resistance to heat, water, and oil. Due to these properties, PFAS have been widely used in various industrial and consumer products. However, PFAS are often referred to as "forever chemicals" because they do not break down easily and can accumulate in the environment and in human and animal tissues over time. This persistence, along with their widespread use, has led to extensive environmental contamination, particularly in water sources.<sup>1</sup> As a result, analyzing PFAS in different matrices, especially drinking water, has become increasingly important. Analyzing PFAS is critical for understanding their distribution, concentration, and potential risks to human health and the environment.<sup>2</sup> To address these health risks, various governmental regulatory agencies have established validated methods to quantify individual or collective PFAS levels in drinking water.&nbsp;</p><h4><strong>Regulatory framework in Europe&nbsp;</strong></h4><p>In Europe, the main regulation governing drinking water quality is the Drinking Water Directive (2020/2184/EU), which sets two thresholds for PFAS:&nbsp;</p><ul><li>0.1 µg/L for the sum of a group of 20 PFAS&nbsp;</li><li>0.5 µg/L for the totality of PFAS<sup>3</sup>&nbsp;</li></ul><p>Some countries, such as Denmark and Italy, test additional compounds. In the United Kingdom, the testing is based on tiered results with different countermeasures depending on PFAS concentrations (Tier 1: &lt;0.01 µg/L, Tier 2: 0.01 µg/L – 0.1 µg/L, Tier 3: &gt;0.1 µg/L). Additionally, several European countries have established limits for the sum of four PFAS compounds to which the population has been most exposed: PFOA, PFNA, PFHxS, and PFOS.<sup>4</sup></p><h4><strong>Analytical approach and methodology&nbsp;</strong></h4><p>Liquid chromatography coupled with mass spectrometry provides high sensitivity and specificity for detecting a wide range of PFAS compounds. In previous work, we demonstrated the ability to meet regulatory requirements for PFAS analysis using large volume injection and high-end triple quadrupole technology for drinking water analysis.<sup>6</sup> Nevertheless, other types of water need sample preparation, which is often critical, to concentrate PFAS from large volumes and to remove potential interferences. We have previously demonstrated the capabilities of an automated dispersive liquid-liquid microextraction (DLLME) procedure using a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=3&amp;search=TriPlus+RSH+SMART"><strong>Thermo Scientific™ TriPlus™ RSH SMART autosampler</strong></a><strong> </strong>for the extraction and analysis of PFAS in drinking water.<sup>7</sup> The DLLME protocol offers advantages such as automation and the use of small sample and reagent volumes. However, the most common approach for PFAS extraction and pre-concentration is based on solid phase extraction (SPE)<sup>8,9</sup> and is compatible with our method.</p><h4><strong>Experimental&nbsp;</strong></h4><h5><strong>Instrument configuration and method&nbsp;</strong></h5><p>A <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/743"><strong>Thermo Scientific™ Vanquish™ Flex Binary UHPLC</strong></a><strong> pump</strong> with a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/742"><strong>Thermo Scientific™ Vanquish™ Duo</strong></a><strong> Autosampler </strong>is used, coupled to a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/404"><strong>TSQ Altis Plus triple quadrupole mass spectrometer</strong></a>. The gradient uses a combination of water and methanol as the mobile phases. The hardware configuration schematics can be seen in Figure 1 and Table 1. A single combination of delay column/ analytical column is used here. The second channel is kept for flexibility for additional or complementary applications. Table 2 recaps the main instrument parameters.</p><h4><strong>Conclusions&nbsp;</strong></h4><p>Our method offers significant advantages for PFAS analysis, ensuring accurate, reliable, and compliant results. The major customer benefit is that we provide a ready-to-go solution, already optimized to meet regulatory limits in terms of performance, designed to comply with regulatory criteria, fully tested for robustness, applicable to any environmental sample type, and extremely easy to handle with a user-optimized interface.&nbsp;</p><ul><li><strong>Accurate PFAS quantitation:</strong> The method enables the precise quantitation of a broad range of PFAS compounds using the targeted approach of the TSQ Altis Plus mass spectrometer, demonstrating high sensitivity, selectivity, and robustness.&nbsp;</li><li><strong>Well-established configuration: </strong>The TSQ Altis Plus configuration is well-established and widely used, ensuring reliability and familiarity.&nbsp;</li><li><strong>Regulatory compliance: </strong>Designed to comply with current European regulations for PFAS in drinking water (0.1 µg/L sum of 20 PFAS; 0.5 µg/L total PFAS); it can be extended to other regional PFAS lists.&nbsp;</li><li><strong>Robust method:</strong> Proven robustness with consistent performance across multiple injections and different matrices (drinking water, surface water, groundwater, wastewater), maintaining accuracy and stability without the need for maintenance or MS tuning.&nbsp;</li><li><strong>Validated performance: </strong>Meets stringent validation criteria, including linearity (R² &gt; 0.990), accuracy, precision, and robustness, with relative amount deviations well within acceptable limits.&nbsp;</li><li><strong>Practical application: </strong>Applicable for the analysis of extracted water samples, including drinking water, surface water, groundwater, and wastewater, supporting environmental and public health monitoring.&nbsp;</li></ul><h3><strong>4. Waters Corporation / ASMS: Automated Cyclic Ion Mobility–Mass Spectrometry MS/MS Workflow and Data Analysis for Shotgun Lipidomics</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009459_e85813f9c2.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p><span>Shotgun lipidomics is a powerful approach for comprehensive lipid profiling, but the identification and quantification of individual lipid species remain challenging due to the presence of isomeric and isobaric compounds. Researchers from the Research Center Borstel – Leibniz Lung Center and Waters Corporation developed an automated workflow combining cyclic ion mobility–mass spectrometry (cIM-MS) with tandem mass spectrometry (MS/MS). By introducing ion mobility separation alongside mass-to-charge ratio (m/z), the approach reduces spectral complexity, improves lipid identification and enables the differentiation of closely related lipid species that conventional mass spectrometry cannot easily resolve.</span></p><p><span>The analytical workflow employed the </span><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/202"><span><strong>Waters SELECT SERIES Cyclic IMS mass spectrometer</strong></span></a><span>, coupled with an Advion Triversa NanoMate nanoelectrospray ionization (nano-ESI) system for direct sample infusion. Lipids were prepared using a modified methyl tert-butyl ether (MTBE) extraction protocol, with five independent extractions performed. Measurements were conducted in positive-ion mode using cyclic ion mobility MS (cIM-MS) and quadrupole-selected cyclic ion mobility tandem MS (Q-cIM-MS/MS). The combination of precursor ion selection, ion mobility separation and fragmentation provided multidimensional information for lipid characterization.</span></p><p><span>An automated data-processing pipeline was developed to handle two-dimensional datasets containing m/z and ion mobility drift-time information. </span><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/635"><span><strong>Waters MassLynx 4.2</strong></span></a><span> and DriftScope 3.0 were used for data acquisition and review, while ApexRT 1.17 supported peak detection. Python libraries, including Pandas and NumPy, facilitated drift-time calculations, and prototype versions of LipidXplorer 1.5 and lxPostman enabled lipid identification, annotation and quantitative data processing. This integrated workflow supported automated feature detection and reduced interference from overlapping lipid signals.</span></p><p><span>The results demonstrated that cyclic ion mobility separation can distinguish overlapping lipid species, including phosphatidylcholines (PC), ether-linked phosphatidylcholines (PC-O), sphingomyelins (SM) and triglycerides (TG). The system acquired 250 Q-cIM-MS/MS measurements within six minutes, highlighting its potential for rapid lipid characterization. In the proof-of-principle experiment, 166 lipid species from eight lipid classes were quantified using positive-ion shotgun lipidomics. The study establishes a promising framework for automated multidimensional lipid analysis, with future developments focusing on expanded MS/MS coverage, integration of positive- and negative-ion measurements and application to larger sample cohorts.&nbsp;</span></p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 45, 2026

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 2nd November 2026? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><h3><strong>1. Agilent Technologies: ICP-MS Elemental Impurity Analysis of Pharmaceuticals Following USP and ICH Protocols</strong></h3><p><strong>Routine oral drug analysis using the </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=10&amp;search=9500"><strong>Agilent 9500 Triple Quadrupole ICP-MS</strong></a><strong> in Advanced Helium Mode</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_elemental_pharma_AHM_9500_icp_ms_5995_0021en_agilent_32a608446d.pdf"><strong>Full PDF for download</strong></a><strong>&nbsp;</strong></li></ul><p>All pharmaceutical products must be evaluated to demonstrate that they are safe for patients and effective for their intended therapeutic use. As part of this evaluation, potentially toxic elemental impurities must be monitored. The relevant requirements and procedures are specified by organizations such as the United States Pharmacopeia (USP) and the International Council for Harmonisation of Technical Requirements (ICH). ICH Q3D(R2) establishes Permitted Daily Exposure (PDE) values for 24 elements and includes the elements specified in USP General Chapter &lt;232&gt;.<sup>1,2</sup> USP General Chapter &lt;233&gt; describes the analytical procedures and validation requirements for determining elemental impurities.<sup>3</sup> In this study, the 24 elements listed in ICH Q3D(R2) were analyzed using an ICP-MS method based on USP &lt;233&gt; to meet the requirements of both ICH and USP.</p><p>Agilent ICP-MS systems, including single quadrupole (SQ) and triple quadrupole (QQQ) instruments, have been extensively tested and used in pharmaceutical laboratories for many years.4 The instruments deliver reliable, high-quality data, supported by trusted service teams, qualification services, and compliance solutions. To extend the benefits of ICP‑QQQ technology to a wider range of laboratories, Agilent has developed the 9500 ICP-QQQ, an entry-level triple quadrupole ICP-MS.<sup>5</sup> Unlike traditional ICP-QQQ systems, which are often used for advanced applications, the 9500 is designed for routine sample analysis. It combines excellent interference removal, dependable performance, and straightforward operation, enabling laboratories to benefit from ICP‑MS/MS capability in high‑volume daily workflows while still supporting more complex research projects.&nbsp;</p><h4><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=10&amp;search=9500"><strong>Agilent 9500 Triple Quadrupole ICP-MS&nbsp;</strong></a></h4><p>The 9500 ICP-QQQ is equipped with the Dual-Cell System (DCS), a new collision/reaction cell (CRC) that incorporates two independently controlled ion guides. The DCS enables effective removal of many spectral interferences through a new standard cell-gas mode, Advanced Helium Mode (AHM), which uses Kinetic Energy Discrimination (KED) and Collision-Induced Dissociation (CID) mechanisms.<sup>6</sup> This enhanced helium (He) mode provides efficient removal of polyatomic interferences while maintaining sufficient high sensitivity in the low-mass range, making it suitable for the analysis of most analytes in the majority of sample types.&nbsp;</p><p>AHM replaces the need for conventional multitune operation using no gas, He, and high energy (HE)He modes. By eliminating gas switching between these modes, AHM simplifies operation and improves sample throughput.&nbsp;</p><p>In pharmaceutical samples, AHM is particularly advantageous for the analysis of elements such as lithium (Li), which typically has low sensitivity in conventional He mode, as well as vanadium (V), chromium (Cr), arsenic (As), and selenium (Se), which are susceptible to polyatomic interferences.&nbsp;</p><p>While AHM effectively resolves many polyatomic ion interferences, such as oxide ions (MO+ ) and argon-based ions (ArM+ ), it is less effective at suppressing doubly charged ion interferences (M++). However, a reactive cell gas mode known as Air cell mode has been developed for the 9500 that reduces M++ interferences through ion–molecule reactions with oxygen (O2 ).7 In Air cell mode, ambient air is purified through an air filter before being introduced into the cell. Using MS/MS settings to control which analyte ions enter the DCS and interact with O2, the 9500 ICP-QQQ ensures effective interference removal of M++, as well as challenging polyatomic species that AHM cannot fully suppress.</p><p>This note describes the analysis of 24 elements in an over-the-counter (OTC) oral drug product using the 9500 ICP-QQQ in AHM. The method was evaluated for both limit and quantitative procedures against the performance criteria specified in the ICH guidance and USP Chapters.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>A rapid method for the simultaneous determination of the 24 elemental impurities specified in ICH Q3D (R2) was successfully developed using the Agilent 9500 Triple Quadrupole ICP-MS. Operating the 9500’s Dual-Cell System (DCS) in Advanced Helium Mode (AHM) effectively suppressed polyatomic interferences while maintaining sufficient sensitivity across the entire mass range. This performance enabled quantification of all target elements in an oral drug product using a single gas mode. Eliminating conventional multitune operation and gas switching also enabled a faster and more streamlined ICP‑MS workflow.&nbsp;</p><p>The method was evaluated in accordance with the requirements of USP &lt;233&gt;, including detectability, precision, intermediate precision, specificity, and accuracy. All 24 elements met the applicable acceptance criteria. The RSDs for the primary isotopes were less than 3% in the precision study, and results obtained on different days met the intermediate precision criterion. Acceptable recoveries were obtained at all evaluated spike levels from 0.3 to 1.5&nbsp;J.&nbsp;</p><p>AHM provided low detection limits and background equivalent concentrations for all 24 elements, including V, Cr, As, and Se, which are susceptible to polyatomic interferences arising from the sample matrix. These results demonstrate effective interference removal capability and quantitative performance of the method. The use of a preset USP/ICH method in the Agilent OpenLab ICP-MS software further simplified method setup and routine operation.&nbsp;</p><p>In addition, Air cell mode provides a complementary massshift approach for specific sample matrices affected by spectral interferences that cannot be sufficiently resolved using AHM alone. Examples include doubly charged rare earth element ion interferences on As and Se.&nbsp;</p><p>Overall, the 9500 ICP-QQQ method using AHM met the requirements of both ICH and USP for the determination of elemental impurities in the OTC drug sample. The ability to accurately determine the target elements at 0.3&nbsp;J confirms that the method is suitable for monitoring elemental impurities at concentrations as low as 30% of the J value.&nbsp;</p><p>The single-mode analytical approach simplifies operation, reduces analysis time, and improves sample throughput, making it suitable for routine pharmaceutical analysis.</p><h3><strong>2. LECO: Metallography of Lithium-Ion Battery Coated Foils</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/METALLOGRAPHY_LITHIUM_ION_BATTERY_COATED_FOILS_203_821_726_ec2c1207dc.pdf"><strong>Full PDF for download</strong></a></li></ul><p>As the demand for renewable energy and green technologies accelerates, companies are prioritizing powerful, efficient, and reliable energy storage solutions. Lithium-ion batteries have rapidly become the leading clean energy choice across automotive, defense, and stationary storage applications. Most lithium-ion cells use a graphite anode paired with either a Lithium Iron Phosphate (LFP) or Lithium Nickel Manganese Cobalt (NMC) cathode. During manufacturing, the anode and cathode slurries are coated onto thin metal foils, which makes metallographic inspection a critical quality control step. Metallography enables precise evaluation of key battery performance factors, including adhesion, coating and foil thickness, and porosity. These characteristics directly influence charge-discharge efficiency, energy density, and overall safety, helping manufacturers prevent defects that could otherwise lead to performance loss or hazardous failures.</p><h4><strong>Understanding Battery Foil Coating&nbsp;</strong></h4><p>Thickness measurements of anode and cathode coatings are essential for preventing downstream issues such as reduced battery efficiency, shortened battery life, and the risk of thermal runaway. Irregular coatings can generate localized hotspots that worsen with every charge– discharge cycle. To minimize manufacturing waste and maintain quality, it is recommended to check coating thickness at multiple stages of the production process.&nbsp;</p><p>These measurements reveal uniformity and flatness across the foil. The material should be evenly distributed with homogeneous particle dispersion and be free of defects such as holes, cracks, delamination, or scratches. By monitoring thickness in real time, manufacturers can adjust parameters, such as coating speed or calendering gaps, to optimize performance and improve overall process efficiency.</p><h4><strong>Image Analysis for Coating Thickness Measurements&nbsp;</strong></h4><p>LECO's image analysis software, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1860"><strong>PAX-it</strong></a>, provides users with an enhanced user interface to automate and generate reports based on common metallographic inspections for coated foils, including coating and layer thicknesses. Samples in the as-polished condition are suitable for observation and these measurements (Figure 7).&nbsp;</p><p>With the “Measurement Sequence” capability, users can repeatedly measure thicknesses from a captured image (Figure 8). The Measurement Sequence is set up to prompt an operator to make measurements based on predefined parameters and locations. It is often recommended to use multiple measurements and areas of a sample for an accurate average of the entire layer of interest (Figure 9). Pass/Fail criteria can be established to alert a user if any of the measurements don't meet quality standards. This can also be used to observe any coating inconsistencies, like porosity or inclusions, that could lead to failure. Users also have the option to customize and print out Excel reports for traceability purposes.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>Metallographic inspection is a critical quality control tool in lithium-ion battery manufacturing, directly impacting performance, safety, and reliability. Accurate evaluation of coating thickness, adhesion, and uniformity on anode and cathode foils helps prevent efficiency losses, premature failure, and safety risks such as thermal runaway. Because these foils are highly sensitive to deformation, careful sectioning, delicate cold mounting, and controlled grinding and polishing are essential to preserve the true microstructure. LECO's recommended preparation methods and consumables enable consistent, deformation-free surfaces suitable for precise analysis. When combined with PAX-it image analysis software, manufacturers can reliably measure coating and foil thickness, detect defects, and generate traceable reports. Overall, a well-controlled metallographic workflow supports optimized process control, reduced manufacturing waste, and the production of safer, higherperforming lithium-ion batteries.</p><h3><strong>3. Metrohm: Automated quality control of diesel using near-infrared spectroscopy</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_nir_080_download_ee71fd0827.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Key parameters for diesel fuel quality testing include the cetane index (ASTM D613), flash point (ASTM D56), density (ASTM D975), T95 (ISO 3405), and viscosity at 40 °C (ISO 3104). Usually, determining these properties is labor intensive and time consuming, requiring multiple analytical techniques and dedicated instrumentation. The OMNIS NIR Analyzer Liquid provides a fast, reliable, and reagentfree solution to simultaneously determine these diesel fuel properties without sample preparation. The precise temperature control ensures that all samples are measured in compliance with ASTM D8340, ASTM D8321, and ASTM D6122. Combined with the OMNIS Sample Robot M – NIR, the system offers fully automated operation for maximum lab efficiency.</p><h4><strong>EXPERIMENTAL EQUIPMENT&nbsp;</strong></h4><p>Diesel samples were measured with an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=220&amp;search=OMNIS+NIR"><strong>OMNIS NIR Analyzer Liquid</strong></a> (Figure 1) in transmission mode over a wavelength range of 1000–2250 nm. Reproducible spectrum acquisition was achieved using the built-in temperature control at 30 °C. An <strong>OMNIS Sample Robot M – NIR </strong>was used for fully automated sample measurement with disposable vials (8 mm pathlength). The vials are closed, which reduces the flammability risk for fuel samples to an absolute minimum. <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=220&amp;search=OMNIS+software"><strong>OMNIS Software</strong></a><strong> </strong>was used for all data acquisition and prediction model development.</p><h4><strong>CONCLUSION</strong></h4><p>This Application Note shows the feasibility of using NIR spectroscopy for the quality control of diesel. Determination of cetane index, cetane number, T95, density, flash point, and viscosity in diesel can be done simultaneously in a few seconds by using the OMNIS NIR Analyzer Liquid. The possibility of fully automated analysis in combination with the OMNIS Sample Robot M – NIR increases throughput, maximizing efficiency. With the OMNIS pre-calibration of diesel (6.06008.009), users can measure all the mentioned parameters from day one.</p><h3><strong>4. Shimadzu: Analysis of Fluorine by EDXRF —A Simple Check for PFAS in Electrical and Electronic Components</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_00977_en_27a7e26bb5.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User benefits</strong></h5><ul><li>EDXRF analyzes halogens (F, Cl, Br, and I) more easily than absorption/emission methods.</li><li>Fluorine (F) on the material surface is detected directly, without sample preparation.</li><li>Simultaneous halogen analysis enables simple screening for the possible use of halogenated flame retardants.</li></ul><p>Asregulations on PFAS and halogenated organic flame retardants tighten in the EU1), 2) and some U.S. states3), 4), there is a growing need to identify at an early stage whether halogens such as fluorine are present in electrical and electronic materials. Rapid supply-chain screening is also increasingly required. The energy dispersive X-ray fluorescence spectrometer <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=1&amp;search=8100"><strong>EDX-8100</strong></a> requireslittle to no sample preparation and can quickly and easily detect halogenssuch asfluorine (F), chlorine (Cl), and bromine (Br). EDX-8100 quickly shows whether these halogens are present, helping narrow down regulated products and prioritize samples for detailed analysis. This article demonstrates the usefulness of halogen detection by EDXRF using an ETFE-coated wire and a relay as examples.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>As shown above, fluorine can be detected and quantified rapidly and easily when its content is about 0.1 wt% or higher. In addition to intentional use, the presence of fluorine compounds can also be confirmed in cases of unintentional inclusion,such as transfer or contamination. In the electrical and electronics field, demand for analysis related to PFAS and halogenated flame retardants is expected to increase in the future, and EDXRF is useful as a simple initial screening method</p>

News from LabRulezICPMS Library - Week 45, 2026

<p><strong>Light and urea. These are two "basic ingredients" that can be enough for effective and gentle water purification. This has been proven by new research conducted by scientists from CEITEC Brno University of Technology (BUT). Although this is only basic research and scientists will continue to build on it, its results can already be used in practice.</strong></p><p>Urea is an organic substance that is commonly found in nature. Thanks to this, it is widely available.</p><p>The key is its modification, in which scientists obtain a special compound of carbon and nitrogen from it. This can then be used for photocatalysis. <i>"Our compound therefore serves as a catalyst that is activated by incident light. Thanks to this, substances that eliminate impurities present in water begin to be released,</i>" says <strong>Daina Arenas Buelvas</strong> from the <i>Quantum Molecular Magnetism</i> team at CEITEC BUT, who led the research.</p><p>This represents a significant shift compared to current methods. Filters that capture impurities are now used to purify water. These filters then need to be removed and the captured substances disposed of. The carbon and nitrogen compound formed from urea, when hit by the light, both captures and decomposes pollutants. In laboratory tests this is done with an efficiency of up to <strong>97%</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Cisteni_vody_pomoci_svetla_Laboratorni_testy_prokazaly_ucinnost_az_97_2_719253ff50.jpeg" alt="CEITEC BUT/Jakub Rozboud:  Purifying Water Using Light? Laboratory Tests Have Shown 97% Efficiency: Daina Dayana Arenas Buelvas preparing samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Cisteni_vody_pomoci_svetla_Laboratorni_testy_prokazaly_ucinnost_az_97_2_719253ff50.jpeg 235w," sizes="100vw" width="1600" height="1067"></p><h3><strong>A unique method that freezes particles</strong></h3><p>The research is also unique thanks to the technology used. The scientists used <strong>EPR spectroscopy</strong>, which can capture free radicals whose lifetime is only a few nanoseconds and “freezes” them, giving scientists the space to examine them.</p><p><i>“This allowed us to describe the entire reaction that took place in the material. We thus obtained information that we and other scientists can use in follow-up research,”</i> points out Daina Arenas Buelvas.</p><blockquote><p>Researchers use a technique called<i> spin trapping</i> to confirm that the material produces reactive oxygen species that break down impurities. Since these molecules are too short-lived to be detected directly, a special 'trap' molecule is added to convert them into a stable, measurable compound, definitively proving that the material generates the active cleaning agents.</p></blockquote><p>The results of the research were <a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S2214993726002150?via%3Dihub"><strong>published</strong></a><strong> </strong>in the prestigious scientific magazine <i>Sustainable Materials and Technologies</i>. In addition to the <i>Quantum Molecular Magnetism</i> team of Vinicius Tadeu Santana from CEITEC BUT, the Brazilian scientific teams of Felipe La Porta from the University of Paraná and Débora Quadreli from the University of Londrina, and the team of Christian M. M. Rocha from the University of Barranquilla, Colombia, also participated in it.</p><h3><strong>They will test the use against microplastics</strong></h3><p>The scientists verified the effectiveness of graphitic carbon nitride, which is the name of the used carbon and nitrogen compound, in water contaminated with methylene blue. Thanks to the photocatalytic reaction, the compound removed 97.2% of the impurities.</p><p>The wide availability of urea and the easy treatment required to obtain the key compound allow for rapid implementation in practice. At the same time, other scientific works can follow up on this research.</p><p>Daina Arenas Buelvas also plans to do the same. <i>"I want to find out whether it is possible to use photocatalysis in graphitic carbon nitride to remove microplastics. But we are only at the beginning," </i>adds the scientist, who came to the Czech Republic from Colombia.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Cisteni_vody_pomoci_svetla_Laboratorni_testy_prokazaly_ucinnost_az_97_3_0b9e23b800.jpeg" alt="CEITEC BUT/Jakub Rozboud:  Purifying Water Using Light? Laboratory Tests Have Shown 97% Efficiency: EPR laboratory at CEITEC BUT." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Cisteni_vody_pomoci_svetla_Laboratorni_testy_prokazaly_ucinnost_az_97_3_0b9e23b800.jpeg 235w," sizes="100vw" width="1600" height="1067"></p><blockquote><h4><strong>Article&nbsp;</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/abs/pii/S2214993726002150?via%3Dihub"><strong>Unraveling precursor effects on the cytotoxicity, adsorption and photocatalytic Performance of g-C3N4 materials: Insights from experimental and computational approaches</strong></a></h4><p>Daina D.A. Buelvas, João P.A. de Jesus, Lucie Kotásková, Christian M.M. Rocha, Débora H. Quadreli, Juliana M. Serpeloni, Glaura S.A. Fernandes, Bruno L.S. Vicentin, Vinicius T. Santana, Felipe A. La Porta&nbsp;</p><p><span><strong>Sustain. Mater. Technol.</strong>, 2026, <strong>48</strong>, e02064</span></p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.susmat.2026.e02064"><span>10.1016/j.susmat.2026.e02064</span></a></p></blockquote>

 Purifying Water Using Light? Laboratory Tests Have Shown 97% Efficiency 

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

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Purifying Water Using Light? Laboratory Tests Have Shown 97% Efficiency

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