Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 23rd February 2026? Check out new documents from the field of spectroscopy/spectrometry and related techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">LabRulezLCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> libraries.</blockquote>This week we bring you application notes by Agilent Technologies, Metrohm, Shimadzu and Thermo Fisher Scientific!<h3>1. Agilent Technologies: UV-Vis Spectroscopy for Quality Assessment of Paprika as per ASTA Method 20.1</h3>From sample prep to analysis using the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=Cary+3500">Agilent Cary 3500 Multicell UV-Vis</a><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_paprika_uv_vis_cary_5994_8612en_agilent_d903914b39.pdf">Full PDF for download</a></li></ul>Paprika is a widely used spice valued for its deep red color and characteristic flavor, contributing to its prominence in both culinary and commercial applications. As a high‑demand commodity produced from dried capsicum pods, paprika is subject to quality variation and adulteration, which can compromise product authenticity, consumer trust, and regulatory compliance. UV-Visible (UV-Vis) spectroscopy is a rapid, reproducible analytical technique for evaluating paprika quality by quantifying pigment concentration. In paprika, color intensity primarily arises from carotenoid compounds, and is commonly assessed using the ASTA Method 20.11 – a well-established industry standard that commercially grades paprika samples as a numerical ASTA color value.In this application note, we used an <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=Cary+3500">Agilent Cary 3500 Multicell UV-Vis spectrophotometer</a> and <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=UV+Workstation+software">Agilent Cary UV Workstation software</a> to determine the ASTA color value of two commercial paprika samples. The measurements were performed in accordance with ASTA Method 20.1 for the determination of extractable color in capsicums and their&nbsp;oleoresins.<h4>Results and discussion</h4><h5>Paprika samples' analysis&nbsp;</h5>ASTA Method 20.1 measures the color intensity of paprika by extracting pigments with acetone and recording absorbance at 460 nm. The absorbance reading, along with sample weight is then used to calculate the ASTA color value. The ASTA color value is a crucial quality parameter for paprika and other spices, as it indicates the intensity and vibrancy of the red color, which is a key characteristic for consumers and food manufacturers. In this study, the two extracted paprika samples were each measured three times to produce triplicate results (Table 4). Sample 1 had a corrected ASTA color value of approximately 103, and sample 2 had a corrected ASTA color value of approximately 61, indicating a lower color intensity. The corrected ASTA color value was automatically generated in an easy-to-read table of values, and a scan report was automatically exported into a user-defined location.<h4>Conclusion&nbsp;</h4>The Agilent Cary 3500 Multicell UV-Vis spectrophotometer with Agilent Cary UV Workstation software enabled fast, reliable, and standardized quality assessment of paprika samples. Using the well-established industry method, ASTA 20.1, the Cary 3500 system provided precise quantification of carotenoid pigments, supporting grading categorization. The high-throughput multicell design of the Cary 3500 Multicell UV-Vis spectrophotometer allows simultaneous measurement of up to seven samples and a reference, improving efficiency and minimizing variability. The Cary UV Workstation software simplifies data acquisition and analysis with intuitive spectral features such as the End of sequence analysis tool, which can be applied automatically to any scan method, and spectra comparisons through the Load spectrum tool. From sample absorbance measurement to final reporting, the Cary 3500 system eliminated the need for manual data processing or external tools, minimizing complexity and facilitating confidence.<h3>2. Metrohm: NIRS analysis of forages</h3>Multiparameter determination in a few seconds<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_nir_131_download_2f897ff7f5.pdf">Full PDF for download</a></li></ul>Forages are plants or parts of plants eaten by both livestock and wildlife. Forages provide bulk, help with weight maintenance, and combat several issues [1]. Alfalfa forage is high in protein—well-suited to promote muscle mass in beef cattle or to increase the production of dairy cows [2]. Alfalfa is also commonly used for horse feed. These animals require forages with good palatability along with high digestibility, intake potential, and protein levels, thus increasing the demand for alfalfa and other high-quality feeds. Farmers have responded by producing even higherquality alfalfa in recent years. Since forage quality depends on chemical, biological, and dynamic properties, both measured and calculated methods must be used. Standard alfalfa assays measure neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), ash, moisture, and protein. For alfalfa pellets, the mean particle size is also important. Near-infrared spectroscopy (NIRS) offers rapid and reliable prediction of fat, moisture, crude protein, fiber, ash and starch in a few seconds and without sample preparation.<h4>EXPERIMENTAL EQUIPMENT</h4>Samples of alfalfa (including fresh alfalfa, alfalfa pellets, and alfalfa cubes) were analyzed on a <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=220&amp;search=NIR+Analyzer">Metrohm NIR Analyzer</a>. All measurements were performed in reflection mode using the large cup. The samples were measured in rotation to collect spectral data from several areas. Spectral averaging of signals from several spots helped to reduce sample inhomogeneity. The reference values were measured according to ISO norms described at the end of this Application Note. Metrohm software was used for all data acquisition and prediction model development.<h4>CONCLUSION&nbsp;</h4>This Application Note demonstrates the feasibility to determine multiple key parameters of alfalfa forage with NIR spectroscopy. Several analytical methods are usually required to measure key quality parameters for forage (Table 4). NIRS forage analysis enables a highly accurate, cost-effective, and fast alternative.<h3>3. Shimadzu: Analysis of Heavy Metals in Baby Food Using ICP-MS</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_01039_en_c563bb1b82.pdf">Full PDF for download</a></li></ul><h4>User Benefits</h4><ul><li>Heavy metals in various baby foods can be analyzed simultaneously with sufficient sensitivity.</li><li>Stable analysis can be performed even for baby foods with high matrix content.</li><li>Using the standard LabSolutions ICPMS rinse function, the time to clean the sample introduction system is minimized, which prevents carryover.</li></ul>Infants’ metabolic pathways are still developing, and because of their lower body weight, they are known to be more susceptible to the effects of harmful substances. In the United States, the Baby Food Safety Act 20211), and in the EU, Commission Regulation (EU) 2023/9152) and Commission Regulation (EU) 2024/19873) require the control of toxic metals contained in baby foods. ICP-MS is suitable for the analysis of trace amounts of toxic metal elements because it can perform multi-element simultaneous analysis with high sensitivity.However, baby foods vary in matrix composition, which raises concerns about non-spectral interferences and long-term stability during ICP-MS analysis. This Application News describes the analysis of five baby foods with different matrices using the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=ICPMS-2040%2F2050">ICPMS-2040/2050</a> (Fig. 1). Additionally, spike recovery tests and long-term stability were assessed based on the Food and Drug Administration (FDA) Elemental Analysis Manual (EAM) Section 4.74).<h4>Equipment Configuration and Analysis Conditions&nbsp;</h4>The equipment configuration is shown in Table 2. To reduce running costs, a mini-torch with lower argon gas consumption compared to conventional torches was used for analysis. Additionally, to streamline sample preparation, an Online Internal Standard Kit was utilized for online addition.&nbsp;The analysis conditions are detailed in Table 3. After analyzing high-concentration samples, the sample introduction system can influence the analysis values of subsequent samples (carryover). If carryover occurs, re-measurement is necessary, which consumes additional time and samples. With LabSolutions ICPMS, rinse solutions can be set for each sample, enabling effective cleaning in minimal time. Normal cleaning was performed using pure water (Rinse solution 1), and an additional acid rinse solution (Rinse solution 2) was introduced only after high-concentration samples.For example, Table 4 shows the difference in carryover concentration when measuring a blank sample after introducing a sample with a CCV concentration, comparing the case where rinse solution 2 is added for an additional 240 seconds to the case where it is not. Among the elements measured this time, Se and Hg tend to have a higher risk of carryover, but by introducing rinse solution 2, carryover was reduced to below the detection limit. Moreover, even if unexpectedly high-concentration samples are introduced, the Extended Rinsing function allows for automatic additional rinsing (Fig. 3). This eliminates the need to repeatedly check analysis values or to set a uniform cleaning time uniformly, thus reducing the verification effort, the amount of rinse solution required, and the analysis time while preventing carryover.&nbsp;Furthermore, to minimize time spent cleaning the sample introduction system, ProActive Rinsing was used (Fig. 4). When measuring multiple samples, starting the rinse in advance during measurement reduces the measurement time for subsequentsamples.<h4>Conclusion&nbsp;</h4>In this Application News, elemental analysis of baby food was conducted using the ICPMS-2040/2050. We achieved detection limits that met stringent standards. The spike recovery tests yielded good results for all samples with different matrices, confirming the validity of the analysis. Additionally, we analyzed 50 samples of infant formula with a high matrix over approximately six hours and confirmed good stability. The ICPMS-2040/2050 offers high sensitivity, accurate analysis, and good stability. Its Extended Rinsing and ProActive Rinsing functions effectively clean the sample introduction system, preventing carryover. Therefore, the ICPMS-2040/2050 is a suitable instrument for elemental analysis in baby food.<h3>4. Thermo Fisher Scientific: Food protection methods assessed by Surface Enhanced Raman Spectroscopy</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/food_protection_methods_surface_enhanced_raman_spectroscopy_an54670_en_5c5e701389.pdf">Full PDF for download</a></li></ul>Lipid oxidation is a major issue for the meat industry as it can not only affect&nbsp;sensory quality in terms of flavor and odor but can also lead to the formation of toxic compounds such as malondialdehyde (MDA) and cholesterol oxidation products, as well as the accumulation of carbonyls, alcohols and volatile acids. The degree of oxidation depends on various factors such as the type of meat, the fat content, the storage temperature, the availability of oxygen and the type of packaging1, 2. To prevent oxidation, synthetic antioxidants are commonly added to meat products, but consumers are now demanding more natural products3. Several studies have been carried out to evaluate the antioxidant activity of many essential oils or extracts from plants or spices such as rosemary, cinnamon or oregano, and the new trend is to develop innovative packaging by adding these natural antioxidants directly to the packaging materials4–6. These materials, often films, are known as “active packaging” because they enable the controlled release of active agents either on the surface or in the internal atmosphere of the product, or by trapping the radicals responsible for harmful effects on food quality. However, the antioxidant performance of these films must be accurately determined to enable the selection of the right packaging for each foodstuff.&nbsp;Among all the techniques available, Raman spectroscopy is a promising candidate for detecting most molecular species with high specificity and sensitivity, particularly in the presence of Surface-Enhanced Raman Scattering (SERS). Indeed, SERS is a physical phenomenon that occurs in the presence of a rough nano-structured metallic surface that causes the intensification of Raman signals from molecules adsorbed to the surface thanks to the combination of a chemical effect and an electromagnetic effect7 . The metals used are generally gold or silver, which can be prepared by electrochemical roughening, metallic coating, or deposition of nanoparticles (often in a colloidal form). Commercial solutions are available, but many researchers prefer to create their own SERS substrates. The scope of this work is to investigate the potential of a home-made SERS substrate to compare the lipid oxidation over time in ground pork preserved either in a low-density polyethylene (LDPE) conventional packaging or in an active packaging containing oregano extract.<h4>Material and method&nbsp;</h4>Fresh ground pork meat was purchased in bulk from a local supermarket and 22 g samples of meat were placed in 5-cm diameter polystyrene Petri dishes. Each sample was covered with an active film and packed in an LDPE bag under a normal atmosphere. Reference samples were also prepared using conventional LDPE films. All samples were stored in a refrigerator at 5 °C and their lipid extracts were analyzed after 0, 7, 9, 11, 14 and 16 days using a home-made SERS substrate based on silver nanoparticles deposited on a glass Petri dish. A special home-made cooling system was used to perform the analyses at low temperature and SERS spectra were measured at -2 °C on a <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2239">Thermo Scientific™ DXR3 Raman Microscope</a> (Waltham, MA, USA) with a 532 nm laser source, a 900 lines/mm grating covering the 50-3500 cm-1 spectral range and an x10 objective (NA 0.40). The laser power was set at 5&nbsp;mW, and 20 exposures of 30 s each were selected to acquire all data. Thermo Scientific™ OMNIC™ v. 9.2 software was used for data collection and analysis. All the measurements were performed in triplicate. As the decrease in the peak at 1655&nbsp;cm-1 (stretching of C=C bonds from unsaturation) can be attributed to lipid peroxidation, and the 1439 cm-1 (scissoring deformation of CH2) is not affected by it, the area ratio between 1655 cm-1 and 1439 cm-1 bands was calculated to follow the lipid oxidation.<h4>Conclusion&nbsp;</h4>The effectiveness of a home-made SERS substrate coupled with a home-made cooling system was demonstrated for monitoring lipid oxidation in packaged ground pork meat over time. The advantages of this technique are its speed and the minimal sample handling requirements. An improvement factor of the order of 107 over conventional Raman was observed. This study also confirmed the greater efficacy of active packaging containing oregano extracts against lipid oxidation in ground pork, compared to conventional LDPE packaging.

News from LabRulezICPMS Library - Week 09, 2026

Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 13th April 2026? Check out new documents from the field of spectroscopy/spectrometry and related techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">LabRulezLCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> libraries.</blockquote>This week we bring you application notes by Metrohm, Shimadzu and Waters Corporation and other document byThermo Fisher Scientific!<h3>1. Metrohm: Dried pet food analysis by nearinfrared spectroscopy (NIRS)</h3>Rapid determination of moisture, protein, fat, and ash<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_nir_129_download_9ff310dbb5.pdf">Full PDF for download</a></li></ul>Pet food testing involves accurate monitoring and control of moisture, protein, fat, and ash content. This allows pet food manufacturers to satisfy nutritional adequacy requirements, meet regulatory guidelines, and provide high-quality foods to pet owners. Traditional analysis methods are often slow and laborintensive. Near-infrared (NIR) spectroscopy provides a fast, nondestructive alternative for analyzing these key quality control parameters with minimal sample preparation. This study evaluates the use of NIR spectroscopy in the pet food industry for efficient and reliable quality control during dried pet food production.<h4>EXPERIMENTAL EQUIPMENT&nbsp;</h4>199 dried pet food samples (mainly dog and cat food) from different suppliers were analyzed in whole and ground forms on an <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=220&amp;search=OMNIS+NIR">OMNIS NIR Analyzer</a> Solid (Figure 1). The samples were added to an OMNIS sample cup and analyzed in diffuse reflection mode. To include sample variety, the sample rotated during measurement to collect spectra from different locations. The automatically averaged spectra were used for model development. Reference values were obtained by official methods such as ISO 6496 (moisture), ISO 5983 (protein), ISO 5984 (ash), and ISO 6492 (fat).<h4>CONCLUSION</h4>This Application Note demonstrates the feasibility of using NIR spectroscopy for the analysis of several pet food quality parameters, including protein, moisture, fat, and ash. Results indicate that lower prediction errors can be obtained when grinding pet food samples before their analysis. A comparison between different methods used for the analysis of pet food clearly highlights the significant time-saving potential of NIR spectroscopy (Table 1). Furthermore, the simplicity of the analysis and the availability of a pre-calibrated instrument, using the pre-calibration, dry pet food, solid (6.06008.027), make both the implementation and the application of NIR spectroscopy with OMNIS NIRS straightforward.<h3>2. Shimadzu: Determination of Elemental Impurities in Bioethanol Using ICP-MS</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_01021_en_942f8087b2.pdf">Full PDF for download</a></li></ul><h4>User Benefits</h4><ul><li>Bioethanol samples can be directly injected into ICP-MS for analysis.</li><li>Trace elements can be accurately determined with high sensitivity.</li></ul>To achieve carbon neutrality, bioethanol is expected to be one of the alternative energy sources to fossil fuels. First-generation bioethanol, produced mainly from corn, sugarcane, and similar crops, has been widely used. However, to avoid competition with food supplies, research and development are also advancing on second-generation bioethanol produced from non-food raw materials such as cellulosic materials. For example in Japan, seven companies including Toyota Motor Corporation have formed the raBit (Research Association of Biomass Innovation for Next Generation Automobile Fuels) to advance technologies for next-generation bioethanol. Shimadzu is also participating as a supporting member to promote R&amp;D through technical cooperation for analysis.&nbsp;Fuels in which bioethanol is blended into gasoline are referred to as E5 (up to 5% bioethanol), E10 (up to 10% bioethanol), etc.; using these fuels in vehicles can reduce environmental impact. At the same time, ethanol blended into gasoline and used as a fuel is required to meet trace element concentration limits (e.g., for copper) specified in standards such as ASTM D48061) and EN 153762). ICP-OES is commonly used for these analyses, but when ultra-trace impurity levels must be measured, higher-sensitivity ICP-MS is more suitable.&nbsp;In this Application, trace elements in first-generation and second-generation bioethanol were determined using the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=ICPMS-2050">ICPMS-2050</a> (Fig. 1). Spike recoveries were also evaluated to confirm the validity of the analytical results.<h4>Conclusion</h4>In this Application, bioethanol was introduced directly into the ICPMS-2050 using an organic solvent introduction system to determine trace elements. Good spike recoveries were obtained, demonstrating that bioethanol can be analyzed accurately by direct injection.<h3>3. Thermo Fisher Scientific: <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2364">Thermo Scientific™ Nicolet™ FTIR</a> and <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2239">Thermo Scientific™ DXR Raman Microscope</a> Solutions</h3><ul><li>Other document</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/FL_52919_microspectroscopy_portfolio_flyer_516ee05816.pdf">Full PDF for download</a></li></ul>Microspectroscopy has become an essential tool in modern analytical laboratories, enabling detailed chemical identification and spatial analysis at the microscale. Whether working in pharmaceuticals, materials science, forensics, or electronics, selecting the right combination of FTIR or Raman microscopy is critical for achieving accurate and efficient results.Thermo Scientific offers a comprehensive portfolio of FTIR and Raman microscope solutions designed to match different analytical needs, user expertise levels, and throughput requirements. From routine QA/QC to advanced research applications, these systems provide scalable solutions for a wide range of analytical challenges .<h4>Matching the Right Tool to Your Application</h4>The portfolio is structured to help users “find their best fit” based on analytical complexity, required spatial resolution, and workflow demands. Entry-level configurations, such as the SurveyIR™ microspectroscopy accessory combined with Nicolet FTIR spectrometers, are ideal for routine identification tasks in QA/QC environments.As analytical needs increase, systems such as the Nicolet iN5 IR microscope with <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2364">Nicolet iS20 FTIR</a> offer improved spatial resolution and flexibility for more frequent analyses. These solutions are particularly suitable for laboratories performing regular identification of materials with moderate complexity.For advanced analytical workflows, integrated systems like the Nicolet iN10 IR microscope or Nicolet RaptIR+™ FTIR microscope provide enhanced capabilities, including spatial distribution analysis and high-resolution measurements down to the micrometer scale. These systems are designed for analytical service labs and R&amp;D environments where both identification and morphological insights are required.<h4>Expanding Capabilities with Raman Microscopy</h4>Raman microscopy complements FTIR by enabling high-resolution chemical imaging and analysis of complex samples. Systems such as the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2239">DXR3 Raman microscope</a> and <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2237">DXR3xi Raman imaging microscope</a> extend analytical capabilities to submicron spatial resolution, making them ideal for advanced research applications.These instruments allow users to not only identify compounds but also visualize their spatial distribution and morphology within a sample. This is particularly valuable in fields such as nanomaterials, semiconductors, and life sciences, where detailed structural and compositional information is required.<h4>From Identification to Imaging</h4>One of the key differentiators across the portfolio is the ability to move from simple identification to comprehensive imaging:<ul><li>Single-point analysis is available across all systems for rapid material identification</li><li>Small-area analysis enables localized chemical characterization</li><li>Large-area and spatial mapping provide insights into distribution and heterogeneity</li></ul>Higher-end systems, such as imaging microscopes, combine these capabilities with advanced automation and data processing tools, allowing users to efficiently analyze complex samples and extract meaningful insights .<h3>4. Waters Corporation: Accurate Particles Characterization in Lipid Nanoparticle Therapies</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009097_en_15af008f22.pdf">Full PDF for download</a></li></ul>Lipid nanoparticles (LNPs) have emerged as one of the most promising drug delivery methods1. LNPs encapsulate genetic material in submicrometer sized lipid vesicles, deliver large biologic payloads, exhibit low immunogenicity, and can enable large manufacturing scalability as seen with the Moderna® and Pfizer® mRNA COVID-19 vaccines. Like all biologics, LNPs can be physically unstable2, aggregate, and form subvisible particles (SVPs), and thus need to be properly formulated and evaluated for physical and chemical stability3. The next generation of LNPs are even more chemically and biologically complex than their predecessors1, presenting unique challenges in both their stability and manufacturability. Accurate low volume subvisible particle measurements will form a critical aspect of the quality assessment of LNPs.&nbsp;Like all injectables, LNPs are subject to USP 787/788 SVP testing4 . However, USP 788 compendial Method 1, light obscuration (LO), is deemed “unsuitable to measure liposomal and colloidal suspensions” as stated in the chapter. In fact, USP 788 recommends membrane microscopy (Compendial Method 2) for measuring liposomal formulations, given LO’s inability to handle solutions with a complex matrix4. Aura uses Backgrounded Membrane Imaging (BMI), a form of membrane microscopy that enables high throughput, low volume subvisible particle characterization. In this application note, we demonstrate how Aura™ GT System can accurately and thoroughly characterize the subvisible particle content of LNPs with volumes as little as 25 µL per sample. We focus on the analysis of two LNP samples formulated in different buffers and subjected temperature and freeze thaw stresses. In addition, we use Aura GT’s SYBR™ Gold assay5 to help characterize nucleic acid escape in stressed LNP samples and quantitatively analyze the stability and purity of these samples.<h4>Experimental</h4><h5>Particle Measurements&nbsp;</h5>LNP samples were measured using BMI and Fluorescence Membrane Microscopy (FMM) using <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=5&amp;search=aura+gt">Aura GT </a>on a black membrane plate. This procedure was adapted for each stress condition three wells were loaded with 25uL per well, and the filtrate stained with 1x SYBR Gold (2% v/v DMSO in PBS) for 1 min prior to FMM measurements. This procedure was adapted from Application note 16.5<h4>Conclusion&nbsp;</h4>Both samples A and B showed significant subvisible particle formation, more than 1x10^6/mL &gt;2 µm across most conditions except thermal stress, pointing to inherently unstable LNP formulations. Freeze thaw cycles significantly increased subvisible particle formation, while heat stress significantly reduced the subvisible particle concentration across both samples. Compared to protein formulations, this heat stress effect may appear unconventional. However, considering the LNP composition and upon further look at the literature, we are able to understand this phenomenon and conclude that significant heat stress can break a liposomal formulation apart3 , increasing the LNP solubility and thereby resulting in less insoluble particles captured on the membrane. On the other hand, freeze thawing produces more insoluble aggregates, thereby increasing subvisible counts as shown in BMI, exhibiting similar behavior to heat induced aggregation as in most protein formulations. A recurring theme in subvisible particle stability is that each formulation exhibits its own particle profile, LNPs are no exception. Another interesting data point is that freeze thawed LNPs showed significant SYBR Gold staining, whereas unstressed LNPs did not, indicating a unique nucleic acid leakage or rupture behavior for this type of stress. Finally, we also observed that buffer type did not significantly impact the subvisible particle count (data not shown). Whilst we observed significant particle counts across all samples tested, pH and buffer variance did not significantly increase particle formation. Indeed, several LNP formulations have reported robust stability profiles across a variety of buffers3 . Filtration of the final product may not be appropriate, as particle formation occurs following filtration, most likely in a concentration dependent manner (Figure 4). Multiple filtration steps, whilst able to clear the product, will undoubtedly result in reduced concentration of pharmacologically active LNPs and as a function of time particles will return as described in our “What Happens When I Filter My Sample” Application Note 21.&nbsp;In conclusion, Aura enables high throughput, low volume, accurate sub-visible particle analysis with direct applicability for LNP stability assessment. Arcane particle analysers, unsuitable for LNP assessment, will struggle to overcome the challenges associated with the physical and chemical complexity of LNPs. Indeed, Aura resolved these key challenges, providing a solution to sub-visible particle analysis of LNPs, from early stage inception through to product release.

News from LabRulezICPMS Library - Week 16, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Restek at ISC 2026

Thermo Fisher Scientific at ISC 2026

Waters at ISC 2026

Managing 4D-STEM data with smarter acquisition

Spatial Proteomics for Translational Research

Streamlining Endotoxin Testing: Automated Sample Preparation and Audit-Ready Workflows for QC Laboratories