This webinar series demonstrates the use of the Thermo Freestyle 1.8 SP2 QF1 software for processing LCMS product-ion mass spectra &nbsp;with the NIST 23 Mass Spectral Search Program (v.3.0). &nbsp;Spectra are easily exported from Freestyle to NIST Search for component identifications utilizing the NIST MSMS libraries and any other available user, crowd-sourced, and commercial MSMS libraries. &nbsp;Freestyle is a very convenient software package for processing data obtained with the their Orbitrap LCMS instruments. &nbsp;In one run both the MS1 and MS2 spectra are obtained in both positive and negative ion modes. &nbsp;The Diode arrary and UV channels can also be easily displayed for targeting the desired species noted in LC analyses.<h5>Handout for whole series</h5><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_1_Data_Processing_My_Favorite_e29bfc0816.pdf">My Favorite Processing Mode in Freestyle</a></li><li>Part 1A: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_1_A_Data_Processing_Preferred_plus_Nearby_Precursors_33bc95778b.pdf">Using "Nearby Precursor" Enhanced Processing Mode in FreeStyle</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_2_Data_Processing_Alternate_99a4b51fa6.pdf">Alternate Processing Modes in Freestyle</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/part_3_improved_handout_d14f81cc32.pdf">Setup of Basic Settings in Freestyle and NIST Search</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_4_NIST_search_type_demonstration_adb3cdc1c5.pdf">Different NIST MSMS Searches Demonstrated</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_5_Elemental_Composition_demonstration_27e151914b.pdf">Elemental Processing within Thermo Freestyle</a></li></ul><h3>Part 1: Data Processing for NIST MSMS Search Using Thermo Freestyle Software (My Favorite Approach)</h3>This guide outlines my preferred method for processing data in Thermo Freestyle software for NIST MSMS searches. These steps streamline data parsing and ensure accurate mass spectral interpretation for LC-MS and MSMS analyses.<h4>Equipment &amp; Software</h4><ul data-spread="false"><li>Thermo Orbitrap MSMS</li><li>Thermo Freestyle Software</li><li>NIST 2023 Tandem Library</li></ul><h4>Orbitrap Data One Acquisition</h4><ul data-spread="false"><li>LC-MS and MSMS Analyses</li><li>Non-targeted acquisition of MSMS</li><li>Positive and Negative Mode</li><li>Photo Detector Array (PDA) and UV-VIS Analyses</li><li>Example compounds:<ul data-spread="false"><li>Uvinul 3000 (MW 214)</li><li>Tinuvin 234 (MW 447)</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_NIST_2023_Tandem_Library_Great_Resource_for_Unknown_Identifications_f4fc13b103.jpg" alt="James Little: NIST 2023 Tandem Library Great Resource for Unknown Identifications" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_NIST_2023_Tandem_Library_Great_Resource_for_Unknown_Identifications_f4fc13b103.jpg 245w," sizes="100vw" width="1384" height="854"><h4>My Favorite Data Processing Workflow</h4><ul><li>shared with me by colleague</li></ul><h5>Step 1: Setting Up Freestyle Windows</h5>To optimize the workspace for data processing:<ol data-spread="false"><li>Open three windows at the bottom of Freestyle:<ul data-spread="false"><li>Top window: Positive ion MS1</li><li>Second window: Negative ion MS2</li><li>Bottom window: UV or PDA trace</li></ul></li><li>Save this layout in Workspace Options/Layouts to ensure automatic parsing when opening new files.</li><li>Add the Nearby Precursors feature to simplify MSMS selection (except for averaged MS spectra).</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Step_1_Setting_Up_Freestyle_Windows_0dac4585f9.jpg" alt="James Little: Step 1 - Setting Up Freestyle Windows" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Step_1_Setting_Up_Freestyle_Windows_0dac4585f9.jpg 245w," sizes="100vw" width="1839" height="403"><h5>Step 2: Adjusting the Window Layout</h5><ol data-spread="false"><li>Use Select Filter to:<ul data-spread="false"><li>Change the top window to positive ion</li><li>Change the second window to negative ion</li><li>Choose PDA or UV for the bottom window</li></ul></li><li>The spectrum window will display the selected MS/MS data for further processing.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Step_2_Adjusting_the_Window_Layout_d18baf8c37.jpg" alt="James Little: Step 2 - Adjusting the Window Layout" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Step_2_Adjusting_the_Window_Layout_d18baf8c37.jpg 245w," sizes="100vw" width="1829" height="1042"><h5>Step 3: Processing MSMS Data</h5><ol data-spread="false"><li>Left-click in the top box for the positive ion chromatogram.</li><li>Right-click to average the spectrum in the Spectrum box.</li><li>Subtract background if necessary before averaging.</li><li>Populate the Avg Range box and select Range 1.</li><li>Go to Positive Ion Chromatogram, specify the range, and confirm.</li><li>Avoid averaging MS2 spectra when exporting to NIST search, as it will convert accurate mass to nominal mass.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Step_3_Processing_MSMS_Data_5a5e2b87b5.jpg" alt="James Little: Step 3 - Processing MSMS Data" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Step_3_Processing_MSMS_Data_5a5e2b87b5.jpg 245w," sizes="100vw" width="1878" height="1059"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Step_3_Processing_MSMS_Data_1_131f4645ca.jpg" alt="James Little: Step 3 - Processing MSMS Data" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Step_3_Processing_MSMS_Data_1_131f4645ca.jpg 234w," sizes="100vw" width="1513" height="1011"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Step_3_Processing_MSMS_Data_2_6ff9040f00.jpg" alt="James Little: Step 3 - Processing MSMS Data" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Step_3_Processing_MSMS_Data_2_6ff9040f00.jpg 245w," sizes="100vw" width="1738" height="1046"><h5>Step 4: Selecting an MS2 Spectrum for NIST Search</h5><ol data-spread="false"><li>Identify an m/z value of interest (e.g., m/z 215.0702).</li><li>Apply Chromatogram range filter to isolate the target ion.</li><li>The MS2 spectrum will replace the MS1 chromatogram in the top window.</li><li>Click on the peak of interest to generate an MS2 spectrum.</li><li>Repeat the process for Negative Ion MS2 (MSMS) spectrum.</li><li>Send spectra to NIST MS search for compound identification.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Step_4_Selecting_an_MS_2_Spectrum_for_NIST_Search_271afd5538.jpg" alt="James Little: Step 4 - Selecting an MS2 Spectrum for NIST Search" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Step_4_Selecting_an_MS_2_Spectrum_for_NIST_Search_271afd5538.jpg 245w," sizes="100vw" width="1849" height="1125"><h3>Conclusion</h3>Using this workflow, Thermo Freestyle software efficiently processes LC-MS/MS data for NIST searches, ensuring accurate identification of unknown compounds. By saving custom layouts and avoiding averaged MS2 spectra, this method maximizes precision and efficiency.

 Data Processing for NIST MSMS Search Using Thermo Freestyle Software (My Favorite Approach)

<h3>Using Artificial Intelligence to Drive MS Workflow Productivity</h3>This is the second in a multi-part blog series highlighting the key attributes and impressive benefits of the Stellar mass spectrometer—the first-of-its-kind instrument designed and optimized for highly multiplexed targeted quantitation, ideally suited—for discovery verification. See Part 1 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5077" aria-label="This is the second in a multi-part blog series highlighting the key attributes and impressive benefits of the Stellar mass spectrometer—the first-of-its-kind instrument designed and optimized for highly multiplexed targeted quantitation, ideally suited—for discovery verification" uw-rm-vague-link-id="https://www.thermofisher.com/blog/analyteguru/why-you-need-a-verification-class-mass-spectrometer/$here" data-uw-rm-vglnk="">here</a> and stay tuned for more blog posts in the series.&nbsp;As artificial intelligence (AI) continues to take the modern world by storm, a similar concept in the scientific world called “automated intelligence” is revolutionizing work for research scientists in their transition from discovery to clinical validation. This version of AI enables you to leverage empirical data, user-defined guidance and instrument performance metrics to manage experiments and dramatically improve productivity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Why_You_Need_a_Verification_class_Mass_Spectrometer_45a584008f.jpg" alt="Thermo Fisher Scientific: Why you need a verification-class mass spectrometer – Part 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Why_You_Need_a_Verification_class_Mass_Spectrometer_45a584008f.jpg 207w," sizes="100vw" width="1024" height="773">It’s possible with an all-new instrument—the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Thermo Scientific Stellar mass spectrometer</a> (MS)—which uses AI to transform the process of building methods, data acquisition management and data processing for interpretation and planning next steps.&nbsp; Combined with the unique instrument performance, the Stellar mass spectrometer creates a new paradigm for targeted quantitation.<h3 id="h-garbage-in-garbage-out">Garbage in, garbage out</h3>Scientific research is all about data, and just like with any computational work, poor-quality input will always produce faulty or unusable output. This idea is especially true for clinical and translational researchers who perform biomarker verification. The quality of your method development and research will always be determined by several things: (1) your existing data (e.g., compound databases, empirical data, in silico predictive models), (2) your guidance (e.g., real-time decision-making parameters) and (3) the experimental capabilities of your instrument platform.<h3 id="h-another-challenge-limitations-in-the-discovery-to-validation-workflow">Another challenge: limitations in the discovery-to-validation workflow</h3>Poor quality input for method development is one challenge, but it’s not the only one. As we discussed in our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5077">first blog post </a>researchers have long struggled with a technology gap in available instrument platforms. Until recently, there were no single-vendor solutions that offered excellent performance for both discovery and targeted verification. This was a serious limitation that impacted how much researchers could rely on AI for method-building. The old process still required a lot more hands-on optimization, which seriously limited productivity.<h3 id="h-finally-automated-intelligence-without-the-drawbacks">Finally: automated intelligence without the drawbacks</h3>With the advent of automated intelligence available on Stellar MS to build targeted methods and manage data acquisition, it’s a whole new ball game. Stellar MS software leverage automated intelligence to boost laboratory productivity, capacity, and lab reputation for high-quality data with faster turnaround times. As a researcher, you can now use automated intelligence to “feed in” your previous work and provide instructions about how to use that information to make decisions and generate new data. Here’s how it works:<h3 id="h-how-ai-speeds-method-creation-and-optimization">How AI speeds method creation and optimization</h3>Stellar MS come with the Thermo Scientific software tool PRM Conductor in Skyline software for automated method building. It uses automated intelligence to build highly effective, targeted acquisition methods from your discovery data (from either<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?instrumentationItems=33&amp;manufacturerItems=3&amp;search=Thermo+Scientific+Orbitrap"> Thermo Scientific Orbitrap</a>-based MS systems or the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Stellar MS</a>).PRM Conductor aids in the generation of targeted-MS2 and MS3 assays. The creation of an assay is broken into three main parts:<ol><li>Skyline metadata describing precursor-to-product transitions is used to filter transitions against a set of thresholds. The filters are based on integrated peak area, relative area, signal-to-background, correlation to median transition, LC base peak width, and retention time. Precursors are filtered by whether they have at least a certain number of qualifying transitions, typically three for peptides and two for small molecules.</li><li>Targeted assays are created with a visualization that depicts the concurrency of the assay in terms of how long the instrument would take to acquire data for the precursors as a function of retention time. Given a desired number of acquired points per LC peak and corresponding cycle time, you can see how various experimental parameters affect the feasibility of acquiring data for the qualifying precursors, such as linear ion trap scan rate and acquisition window width.</li><li>Finally, you can decide whether to create multiple assays for all qualifying precursors, or a single assay with a “balanced load” that picks the best N peptides per protein. Instrument methods for the final assay(s) can then be exported, based on a user-defined template method.</li></ol>In addition, Stellar MS solutions use <a target="_blank" rel="noreferrer noopener" aria-label="Normalized Collision Energy - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://assets.thermofisher.com/tfs-assets/cmd/warranties/psb104-normalized-collision-energy-technology-en.pdf$normalizedcollisionenergy"><a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Warranties/PSB104-Normalized-Collision-Energy-Technology-EN.pdf">Normalized Collision Energy</a></a> (NCE) – a well-established principle that automatically compensates for mass dependency by automatically estimating all of the collision energies. NCE bypasses the need for optimization per precursor-to-product ion transition performed by a triple quadrupole mass spectrometer which takes much longer to perform, multiplies the experimental cost due to replicate injections of standards and dedicated research staff time.&nbsp;<h3 id="h-how-ai-streamlines-data-acquisition">How AI streamlines data acquisition</h3>With the Stellar MS automated intelligence capabilities, scientists can also speed data acquisition with these tools:Dynamic AGC (automated gain control) helps the system maintain constant ion count and high data quality regardless of ion flux. Essentially, the Stellar MS regulates the number of charges or ions per spectrum by employing a hyper-fast ion gate managing ion accumulation to pass an ideally constant number of ions per spectrum.<figure class="image"><img style="aspect-ratio:400/443;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Why_you_need_a_verification_class_mass_spectrometer_Part_2_2db3d7d0df.jpg" alt="Thermo Fisher Scientific: Figure 1: a) Theoretical depiction of how AGC expands the accessible quantitative range of concentrations. b) Assessment of active targets at the start of a cycle, with their minimum acquisition times on the y-axis, and where more intense precursors are white. 3) Expansion of injection times inverse-proportionally to target abundance. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Why_you_need_a_verification_class_mass_spectrometer_Part_2_2db3d7d0df.jpg 141w," sizes="100vw" width="400" height="443"></figure>As depicted in Figure 1, the ability to vary the injection time expands the dynamic range of quantitation, from a base range given by just the storage and detector saturation limits and multiplies it by the gate linearity range. In the case of the Stellar MS, the pulsed nature of ion trap mass analysis limits the intra-scan dynamic range to about three orders (from 1 to 25k ions in a peak), while the gate can give a linear response from about 3d-6 seconds up to a practical experimental limit, for example 3e-1 seconds.Another tool, Adaptive RT, dynamically manages PRM acquisition, which means you’ll nearly eliminate the risk of missing data while maximizing target capacity.&nbsp; Missing data requires you to re-run samples, costing time and money. Using the method (generated by PRM Conductor software), you can run one representative sample on the gradient and product ion distribution profiles as a function of retention time are built into the instrument editor, which combines PRM settings and Adaptive RT to manage every microsecond of sample acquisition. The system will continue to update and modify the method to adjust for any unexpected LC perturbation or manage data acquisition across multi-channel UHPLC column formats. For a more in-depth description, check out the <a target="_blank" rel="noreferrer noopener" aria-label="newly published BioRxiv paper - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.biorxiv.org/content/10.1101/2024.05.31.596848v1$newlypublishedbiorxivpaper"><a target="_blank" rel="noopener noreferrer" href="https://www.biorxiv.org/content/10.1101/2024.05.31.596848v1">newly published BioRxiv paper</a></a>.<h3 id="h-conclusion-automated-intelligence-maximizes-workflow-performance">Conclusion: Automated intelligence maximizes workflow performance</h3>In order to maximize LC-MSn workflow performance for translational verification, you need solutions that streamline all steps while meeting experimental expectations—scale, sensitivity, specificity, throughput, and ease-of-use. Together, automated intelligence on the Stellar MS combined with unique instrument performance can help deliver the ultimate performance to boost your lab’s productivity and prestige.<blockquote>For more information, explore the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Stellar Mass Spectrometer MS</a> or <a target="_blank" rel="noreferrer noopener" aria-label="reach out to your Thermo Fisher Scientific sales representative - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.thermofisher.com/us/en/home/global/forms/industrial/stellar-mass-spectrometer-contact.html$reachouttoyourthermofisherscientificsalesrepresentative"><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/global/forms/industrial/stellar-mass-spectrometer-contact.html">reach out to your Thermo Fisher Scientific sales representative</a></a>.Intrigued to learn more about the Stellar MS and how it can improve your work? Watch for more blog posts in this series soon.<h5>Visit our&nbsp;<a target="_blank" rel="noreferrer noopener" aria-label="LinkedIn page - open in a new tab" data-uw-rm-ext-link="" uw-rm-external-link-id="https://www.linkedin.com/showcase/chromatography-&amp;-mass-spectrometry/posts/?feedview=all$linkedinpage"><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/chromatography-&amp;-mass-spectrometry/posts/?feedView=all">LinkedIn page</a></a>: #StellarMassSpec #MassSpectrometry #TranslationalResearch</h5><h4>Dr. Scott Peterman</h4><div itemprop="description">Scott Peterman, Ph.D., is Senior Product Marketing Manager for Life Science Mass Spectrometry of the Chromatography and Mass Spectrometry Division of Thermo Fisher Scientific.</div></blockquote>

Why you need a verification-class mass spectrometer – Part 2

The analysis of intact proteins, such as therapeutic monoclonal antibodies (mAbs) under “native” (non-denaturing) conditions using mass spectrometry gives drug developers unique structural insights and allows simplified determination of some biochemical and biophysical properties.Until recently these analyses have been the domain of time-of-flight (TOF) mass spectrometers, due to the ability of this analyzer type to determine masses of very high mass-to-charge ratio (m/z).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_TOF_vs_Orbitrap_for_Intact_Protein_Structural_Insights_a3206fa950.jpg" alt="Thermo Fisher Scientific: TOF vs. Orbitrap for Intact Protein Structural Insights." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_TOF_vs_Orbitrap_for_Intact_Protein_Structural_Insights_a3206fa950.jpg 245w," sizes="100vw" width="768" height="414">Systems conventionally required specialized configurations and conditions to ensure large ions were de-solvated efficiently in the source, transported through the mass spectrometer with adequate transmission efficiency, and detected with acceptable selectivity such that all relevant protein isoforms can be differentiated and identified.TOF systems used for this work typically required expert tuning to achieve acceptable results; however many systems suffered robustness in source de-solvation and lacked spectral resolution to separate key proteoforms. TOF-based systems could provide average intact and native mass, but features such as glycoforms were lost in the spectral noise – TOF could see the forest, but not the trees.<h3 id="toc-hId--1902366970" data-uw-rm-heading="level" role="heading" aria-level="2">See the Detail</h3>Consequently, significant efforts were made in the development of extended mass range Orbitrap-based mass spectrometers for the characterization of intact protein assemblies. In 2012, a prototype <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/iqlaaegaapfalgmbdl?ce=E.19CMD.PM102.16927.01&amp;cid=E.19CMD.PM102.16927.01">Thermo Scientific Exactive Plus EMR system</a> was used by <a target="_blank" rel="noopener noreferrer" href="https://www.ncbi.nlm.nih.gov/pubmed/23064518">Rose et al.</a>, to determine measurement of various protein assemblies with “outstanding mass-spectral resolution.”Data on the Orbitrap-based system represented a “notable improvement over those obtained with TOF instrumentation.” TOF data was only able to determine an average m/z result for large m/w proteins; whereas data obtained using orbitrap-based MS was baseline resolved; which even allowed determination of binding events on these large proteins. The improvements in spectral resolution on Orbitrap meant a new level of structural insight could be gained, even to the level of studying small molecule interactions with megadalton proteins – studies that were previously unimaginable using TOF.High mass analysis Orbitrap-based MS development didn’t stop there; in 2017 Thermo Fisher Scientific launched a <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/order/catalog/product/0726055?ce=E.19CMD.PM102.16927.01&amp;cid=E.19CMD.PM102.16927.01">BioPharma Option</a> for the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/uk/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-hybrid-mass-spectrometer-comparison.html?ce=E.19CMD.PM102.16927.01&amp;cid=E.19CMD.PM102.16927.01">Thermo Scientific Q Exactive range of mass spectrometers</a>. This enabled a high mass mode for determinations up to 8,000 m/z, opening the door for simple, robust, and high spectral quality intact mass analyses of mAbs. Not only did this provide exceptional data to drug developers, it also came with a simple and robust set-up procedure that made acquisition of data under native-like conditions as simple as acquiring a peptide map.Drug researchers like <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cs_72645_native_ms_protein_characterization_omass_cs72645_en_d12100345a/cs-72645-native-ms-protein-characterization-omass-cs72645-en.pdf">OMass</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/cs_72665_ms_therapeutic_proteins_lifearc_cs72665_en_cfa26cdc5d/cs-72665-ms-therapeutic-proteins-lifearc-cs72665-en.pdf">LifeArc</a> and <a target="_blank" rel="noopener noreferrer" href="http://analyteguru.com/how-to-make-cancer-face-the-music/">Symphogen</a> were quick to adopt this technology in routine.<blockquote>“The system gets tuned and calibrated once a week. It’s incredibly stable and easy to set-up.” – Dr. David Tickle, Senior Principal Scientist, LifeArc</blockquote><h3 id="toc-hId--1873737819"><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=xtj_JIcD9BM">Symphogen on TOF vs. Orbitrap</a></h3>Users were able to run native-MS every day, and generate data with spectral quality so superior that they are now able to achieve full intact protein glycoform determinations in 15 minutes using Orbitrap-based analysis. This was a process that conventionally required complex sample preparation and chromatographic fractionation routines when using TOF based detection.<blockquote>“The thing that struck me is the difference in quality of data when comparing TOF vs Orbitrap data.”&nbsp; – Dr. Dan Bach Kristensen, Principal Investigator, Symphogen</blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_TOF_vs_Orbitrap_for_Intact_Protein_Structural_Insights_2_98f57e9e65.jpg" alt="Thermo Fisher Scientific: TOF vs. Orbitrap for Intact Protein Structural Insights." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_TOF_vs_Orbitrap_for_Intact_Protein_Structural_Insights_2_98f57e9e65.jpg 245w," sizes="100vw" width="990" height="396"><h3 id="toc-hId--1845108668"><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=CE2O4a0VJaw">CV-MS for Simple Characterization of Native Variants</a></h3><h3 id="toc-hId--1816479517">We want more!</h3>When it comes to intact and native mass spectrometry average mass is no longer enough. Structural insights are only provided when isoforms are easily differentiated and can be confidently determined. Orbitrap-based mass spectrometry delivers.So much so that researchers are wanting to analyse ever-larger and more complex molecules. This led, last year, to the introduction of the new <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/us/en/home/products-and-services/promotions/industrial/q-exactive-uhmr.html?ce=E.19CMD.PM102.16927.01&amp;cid=E.19CMD.PM102.16927.01">Thermo Scientific Q Exactive UHMR</a> (Ultra High Mass Range) Hybrid Quadrupole-Orbitrap MS System. The Q Exactive UHMR systems enable analysis of intact MegaDalton assemblies and resolve small differences in masses that reveal key ligands, modifications and interactions for better understanding of biological processes.<blockquote>“Thermo Fisher has made a major contribution to native mass spectrometry in recent years. In the past we couldn’t get resolution of very large protein complexes with few charges. This was a real stumbling block for us. We have benefited from recent developments in native high-resolution mass spectrometry. We didn’t realize what we weren’t seeing before.”&nbsp; – Professor Dame Carol Robinson, Chief Scientific Consultant, OMass Technologies</blockquote>For more information on Orbitrap-based mass spectrometry for intact and native mass analysis <a target="_blank" rel="noopener noreferrer" href="http://www.thermofisher.com/nativems?ce=E.19CMD.PM102.16927.01&amp;cid=E.19CMD.PM102.16927.01">click here.</a>

TOF vs. Orbitrap for Intact Protein Structural Insights

This webinar series demonstrates the use of the Thermo Freestyle 1.8 SP2 QF1 software for processing LCMS product-ion mass spectra &nbsp;with the NIST 23 Mass Spectral Search Program (v.3.0). &nbsp;Spectra are easily exported from Freestyle to NIST Search for component identifications utilizing the NIST MSMS libraries and any other available user, crowd-sourced, and commercial MSMS libraries. &nbsp;Freestyle is a very convenient software package for processing data obtained with the their Orbitrap LCMS instruments. &nbsp;In one run both the MS1 and MS2 spectra are obtained in both positive and negative ion modes. &nbsp;The Diode arrary and UV channels can also be easily displayed for targeting the desired species noted in LC analyses.<h5>Handout for whole series</h5><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_1_Data_Processing_My_Favorite_e29bfc0816.pdf">My Favorite Processing Mode in Freestyle</a></li><li>Part 1A: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_1_A_Data_Processing_Preferred_plus_Nearby_Precursors_33bc95778b.pdf">Using "Nearby Precursor" Enhanced Processing Mode in FreeStyle</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_2_Data_Processing_Alternate_99a4b51fa6.pdf">Alternate Processing Modes in Freestyle</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/part_3_improved_handout_d14f81cc32.pdf">Setup of Basic Settings in Freestyle and NIST Search</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_4_NIST_search_type_demonstration_adb3cdc1c5.pdf">Different NIST MSMS Searches Demonstrated</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_5_Elemental_Composition_demonstration_27e151914b.pdf">Elemental Processing within Thermo Freestyle</a></li></ul><h3>Part 4: Data Processing for NIST MSMS Search using Thermo Freestyle Software (NIST Search Type Demonstration)</h3><h4>Equipment &amp; Software</h4><ul><li>Thermo Orbitrap MSMS</li><li>Thermo Freestyle Software</li><li>NIST 2023 Tandem Library</li></ul><h4>Streamlining Data Processing for Mass Spectrometry</h4>Mass spectrometry is a powerful tool for compound identification, but its efficiency depends largely on how data is processed and analyzed. By utilizing Thermo Freestyle Software in combination with NIST MSMS Search, researchers can enhance the accuracy and speed of their spectral identifications.<h4>NIST MSMS Search v3.0 – Simplified and Enhanced</h4>The NIST MSMS Search version 3.0 introduces an improved interface, making search settings more intuitive. This version allows for:<ul><li>Spectrum import flexibility – Multiple ways to import and process spectra</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Typical_Settings_for_NIST_MS_Search_3_0_Tandem_Mode_dc120f16ec.jpg" alt="James Little: Typical Settings for NIST MS Search 3.0 (Tandem Mode)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Typical_Settings_for_NIST_MS_Search_3_0_Tandem_Mode_dc120f16ec.jpg 245w," sizes="100vw" width="1574" height="664"><ul><li>Various search modes<ul><li>Identity MS/MS: searches spectra restricting by the precursor ion and its specified m/z</li><li>Identity/High Resolution: No Precursor, just compares the overall fit of unknown spectra to reference spectra</li><li>Similarity/Hybrid MS/MS - very useful if a related compound is present in the reference libraries, but the actual compound is not present</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Library_Search_Options_for_NIST_MS_Search_3_0_Tandem_Mode_bb8ca30d1e.jpg" alt="James Little: Library Search Options for NIST MS Search 3.0 (Tandem Mode)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Library_Search_Options_for_NIST_MS_Search_3_0_Tandem_Mode_bb8ca30d1e.jpg 245w," sizes="100vw" width="1569" height="786"><ul><li>Advanced filtering options – Remove low-intensity ions and electronic noise</li><li>Customizable settings – Users can save configurations for future searches</li></ul><h4>Configuring Search Settings for Optimal Performance</h4>To get the most accurate results, users should:<ul><li>Select the appropriate MSMS libraries – NIST 2023 includes essential databases.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Select_the_appropriate_MSMS_libraries_42e991834a.jpg" alt="James Little: Select the appropriate MSMS libraries" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Select_the_appropriate_MSMS_libraries_42e991834a.jpg 199w," sizes="100vw" width="1036" height="814"><ul><li>Adjust precursor m/z tolerance – Ensure compatibility with your mass spectrometer.</li><li>Use the “Best Matching” option – Simplifies results by filtering only the best spectrum using the CAS Number as a filter</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Other_Settings_for_NIST_MS_Search_3_0_Tandem_Mode_3b222cdcf4.jpg" alt="James Little: Other Settings for NIST MS Search 3.0 (Tandem Mode)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Other_Settings_for_NIST_MS_Search_3_0_Tandem_Mode_3b222cdcf4.jpg 245w," sizes="100vw" width="1575" height="869"><ul><li>Save search configurations – Avoid resetting parameters for each new session.</li></ul>⚠️ Important! Save your settings for future searches using the Save Configuration option, and restore them when needed to maintain consistency in your analyses.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Important_Save_Your_Settings_for_Future_Sessions_f614f0a27e.jpg" alt="James Little: Important! Save Your Settings for Future Sessions." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Important_Save_Your_Settings_for_Future_Sessions_f614f0a27e.jpg 245w," sizes="100vw" width="1072" height="632"><h4>Conclusion</h4>Utilizing NIST MSMS Search v3.0 within Thermo Freestyle Software allows for a faster, more accurate, and customizable approach to compound identification. By selecting the appropriate search type and optimizing settings, researchers can enhance the reliability of their mass spectrometry workflows.

Data Processing Using Thermo Freestyle Software with NIST MSMS Search for Compound Identifications (NIST Search Type Demonstration)

Quantification of Gd species in a tetrameric Gd-based contrast agent using HPLC-ICP-MS

This webinar series demonstrates the use of the Thermo Freestyle 1.8 SP2 QF1 software for processing LCMS product-ion mass spectra &nbsp;with the NIST 23 Mass Spectral Search Program (v.3.0). &nbsp;Spectra are easily exported from Freestyle to NIST Search for component identifications utilizing the NIST MSMS libraries and any other available user, crowd-sourced, and commercial MSMS libraries. &nbsp;Freestyle is a very convenient software package for processing data obtained with the their Orbitrap LCMS instruments. &nbsp;In one run both the MS1 and MS2 spectra are obtained in both positive and negative ion modes. &nbsp;The Diode arrary and UV channels can also be easily displayed for targeting the desired species noted in LC analyses.<h5>Handout for whole series</h5><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/4978">My Favorite Processing Mode in Freestyle</a></li><li>Part 1A: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_1_A_Data_Processing_Preferred_plus_Nearby_Precursors_33bc95778b.pdf">Using "Nearby Precursor" Enhanced Processing Mode in FreeStyle</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_2_Data_Processing_Alternate_99a4b51fa6.pdf">Alternate Processing Modes in Freestyle</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/part_3_improved_handout_d14f81cc32.pdf">Setup of Basic Settings in Freestyle and NIST Search</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5160">Different NIST MSMS Searches Demonstrated</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/Part_5_Elemental_Composition_demonstration_27e151914b.pdf">Elemental Processing within Thermo Freestyle</a></li></ul><h3>Part 1A: Data Processing Using Thermo Freestyle Software with NIST MSMS Search for Compound Identifications (“Nearby Precursors” Enhancement)</h3><h4>Equipment &amp; Software</h4><ul><li>Thermo Orbitrap MSMS</li><li>Thermo Freestyle Software</li><li>NIST MSMS Search</li></ul>Mass spectrometry data processing is a critical step in analyzing complex chemical mixtures. This article focuses on a preferred method for processing data using Thermo Freestyle software, with an added enhancement—Nearby Precursors. This approach allows for efficient spectral analysis and identification of compounds, improving workflow efficiency and accuracy.<h3>Step-by-Step Guide to Data Processing</h3><h4>1. Setting Up the Workspace</h4>To streamline data analysis, follow these steps to configure the workspace in Thermo Freestyle:<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Open three windows at the bottom of Freestyle:&nbsp;<ul style="list-style-type:circle;"><li style="tab-stops:list 72.0pt;">Top Window: Positive ion MS1</li><li style="tab-stops:list 72.0pt;">Middle Window: Negative ion MS2</li><li style="tab-stops:list 72.0pt;">Bottom Window: UV or PDA trace</li></ul></li><li style="tab-stops:list 36.0pt;">Save the layout using Workspace Options/Layouts to ensure automatic application when new files are opened.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Part_1_A_Setting_Up_the_Workspace_e21f14fc45.jpg" alt="James Little: Part 1A - Setting Up the Workspace" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Part_1_A_Setting_Up_the_Workspace_e21f14fc45.jpg 245w," sizes="100vw" width="1558" height="386"><h4>2. Selecting Ranges and Filters</h4><ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Keep adding windows until you have three total.</li><li style="tab-stops:list 36.0pt;">Use the filter function to configure:&nbsp;<ul style="list-style-type:circle;"><li style="tab-stops:list 72.0pt;">Positive ion in the top window</li><li style="tab-stops:list 72.0pt;">Negative ion in the second window</li><li style="tab-stops:list 72.0pt;">UV/PDA trace in the bottom window</li></ul></li><li style="tab-stops:list 36.0pt;">The positive and negative ion options are the last two items in the filter list.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Part_1_A_Selecting_Ranges_and_Filters_34d956ade6.jpg" alt="James Little: Part 1A - Selecting Ranges and Filters" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Part_1_A_Selecting_Ranges_and_Filters_34d956ade6.jpg 245w," sizes="100vw" width="1486" height="790"><h4>3. Spectrum Window and Data Processing</h4>Once the workspace is set up, it provides three working areas:<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Processing boxes to refine data selection</li><li style="tab-stops:list 36.0pt;">Function selection boxes to choose necessary analyses</li><li style="tab-stops:list 36.0pt;">Spectrum visualization for MS and MSMS data</li></ul>Tip: Always save the layout so it automatically loads when opening new files.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Part_1_A_Spectrum_Window_and_Data_Processing_d0b532ee4d.jpg" alt="James Little: Part 1A - Spectrum Window and Data Processing" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Part_1_A_Spectrum_Window_and_Data_Processing_d0b532ee4d.jpg 245w," sizes="100vw" width="1500" height="859"><h3>Enhancing Analysis with Nearby Precursors</h3>The Nearby Precursors feature allows users to quickly access and analyze MS2 spectra from associated peaks. Here’s how to use it effectively:<h4>1. Selecting Peaks and Generating MS2 Spectra</h4><ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Left-click on a peak in either the positive or negative chromatogram (e.g., peak at 10.05 minutes).</li><li style="tab-stops:list 36.0pt;">The corresponding MS2 spectrum will appear in the second window.</li><li style="tab-stops:list 36.0pt;">Double left-click on the purple circle associated with the ion to display the MSMS spectrum.</li></ul>Note: Nearby Precursors cannot be used with an averaged MS spectrum!<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Part_1_A_Nearby_Precursors_Option_2435bc6a66.jpg" alt="James Little: Part 1A - Nearby Precursors Option" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Part_1_A_Nearby_Precursors_Option_2435bc6a66.jpg 245w," sizes="100vw" width="1169" height="633"><h4>2. Sending Spectra to NIST MSMS Search</h4>Once the MS2 spectrum is displayed:<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Select the bar above the spectrum with a left-click.</li><li style="tab-stops:list 36.0pt;">Send the spectrum to NIST search for further identification.</li><li style="tab-stops:list 36.0pt;">Remove unwanted spectra by clicking the ‘x’ in the box.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Part_1_A_Nearby_Precursors_Option_NIST_MSMS_74e962efcd.jpg" alt="James Little: Part 1A - Nearby Precursors Option - NIST MSMS" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Part_1_A_Nearby_Precursors_Option_NIST_MSMS_74e962efcd.jpg 245w," sizes="100vw" width="1477" height="673"><h4>Troubleshooting: Recovering Deleted Spectra Windows</h4>If all MS and MS2 spectrum windows are accidentally removed:<ol><li style="tab-stops:list 36.0pt;">Navigate to Workspace Options &gt; Spectrum &gt; Insert View.</li><li style="tab-stops:list 36.0pt;">Reinsert the necessary spectrum windows to continue the analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Part_1_A_If_You_Delete_All_the_MS_and_MS_2_Windows_by_Mistake_b62af649db.jpg" alt="James Little: Part 1A - If You Delete All the MS and MS2 Windows by Mistake" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Part_1_A_If_You_Delete_All_the_MS_and_MS_2_Windows_by_Mistake_b62af649db.jpg 205w," sizes="100vw" width="998" height="758"><h4>Conclusion</h4>The Preferred Approach combined with the Nearby Precursors enhancement provides a streamlined and efficient way to process LC-MS and MSMS data using Thermo Freestyle software. This method ensures better spectral organization, easier peak selection, and faster searches in NIST MSMS databases.

Data Processing Using Thermo Freestyle Software with NIST MSMS Search for Compound Identifications (“Nearby Precursors” Enhancement)

This webinar series demonstrates the use of the Thermo Freestyle 1.8 SP2 QF1 software for processing LCMS product-ion mass spectra &nbsp;with the NIST 23 Mass Spectral Search Program (v.3.0). &nbsp;Spectra are easily exported from Freestyle to NIST Search for component identifications utilizing the NIST MSMS libraries and any other available user, crowd-sourced, and commercial MSMS libraries. &nbsp;Freestyle is a very convenient software package for processing data obtained with the their Orbitrap LCMS instruments. &nbsp;In one run both the MS1 and MS2 spectra are obtained in both positive and negative ion modes. &nbsp;The Diode arrary and UV channels can also be easily displayed for targeting the desired species noted in LC analyses.<h5>Content of previous episodes:</h5><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/4978">My Favorite Processing Mode in Freestyle</a></li><li>Part 1A: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5211">Using "Nearby Precursor" Enhanced Processing Mode in FreeStyle</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Part_2_Data_Processing_Alternate_99a4b51fa6.pdf">Alternate Processing Modes in Freestyle</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/part_3_improved_handout_d14f81cc32.pdf">Setup of Basic Settings in Freestyle and NIST Search</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5160">Different NIST MSMS Searches Demonstrated</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/Part_5_Elemental_Composition_demonstration_27e151914b.pdf">Elemental Processing within Thermo Freestyle</a></li></ul><h3>Part 2: Data Processing Using Thermo Freestyle Software with NIST MSMS Search for Compound Identifications (Alternative "Less Efficient" Approaches)</h3><h4>Equipment &amp; Software</h4><ul><li>Thermo Orbitrap MSMS</li><li>Thermo Freestyle Software</li><li>NIST MSMS Search</li></ul><h4>Efficient Compound Identification Using Thermo Orbitrap MSMS</h4>In this technical guide, James Little outlines methods for processing mass spectrometry data using Thermo Freestyle Software in combination with NIST MSMS Search. The focus is on interpreting data collected from Thermo Orbitrap LC-MS systems operating in both positive and negative ionization modes, supported by Photo Diode Array (PDA) and UV-VIS detection.<h4>Preferred Data Processing Workflow</h4>While there are multiple approaches available in Freestyle, the guide emphasizes a preferred method shared by a colleague — a workflow found to be more efficient once initial setup is completed.Key Steps:<ul><li>Open the data file to view the Total Ion Chromatogram (TIC).</li><li>Left-click to select a peak, then use keyboard arrow keys to “walk through” the chromatographic peak.</li><li>Refer to the header in the lower spectrum display to check polarity and MS1/MS2 status.</li><li>Send the desired MS2 spectrum to NIST MSMS Search for compound identification.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Windows_Arrow_Key_Approach_Using_Total_Ion_Chromatogram_NOT_Parsed_into_Separate_Ones_c089074c5f.jpg" alt="James Little: Windows Arrow Key Approach Using Total Ion Chromatogram, NOT Parsed into Separate Ones" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Windows_Arrow_Key_Approach_Using_Total_Ion_Chromatogram_NOT_Parsed_into_Separate_Ones_c089074c5f.jpg 245w," sizes="100vw" width="1627" height="825"><blockquote>This arrow-key approach leverages the TIC directly without separating it into multiple parsed chromatograms.</blockquote><h4>Alternate Manual Workflow</h4>If not using the keyboard-based approach, a more manual method involves:<ul><li>Clicking the Scan Filters Icon in the Freestyle Workspace &gt; Processing Tab.</li><li>Selecting from the MS1 positive and negative ion lists at the bottom.</li><li>Inserting traces (e.g., PDA or UV) via the Insert Trace option.</li><li>Accessing the PDA/UV traces through the Detector Tab.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Manually_Opening_Windows_e4d5cab7fc.jpg" alt="James Little: Manually Opening Windows" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Manually_Opening_Windows_e4d5cab7fc.jpg 245w," sizes="100vw" width="1619" height="906"><h4>Compound Example: Uvinul 3000</h4><ul><li>Molecular Weight: 214</li><li>Demonstrated using Thermo Orbitrap with both LC-MS and non-targeted MSMS Analyses (Positive and Negative Modes) with Photo Array Detector (PDA) and UV-VIS Analyses</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/James_Little_Uvinul_3000_b0caa75d37.jpg" alt="James Little: Uvinul 3000" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_James_Little_Uvinul_3000_b0caa75d37.jpg 230w," sizes="100vw" width="464" height="315">

Data Processing Using Thermo Freestyle Software with NIST MSMS Search for Compound Identifications (Alternative "Less Efficient" Approaches)

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