Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 29th September 2025? Check out new documents from the field of spectroscopy/spectrometry and related techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">LabRulezLCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> libraries.</blockquote>This week we bring you application note by Agilent Technologies and posters by &nbsp;Shimadzu / AOAC and Thermo Fisher Scientific!<h3>1. Agilent Technologies: Accurate Identification of Binder Raw Materials for Li-Ion Battery Electrodes by FTIR</h3>Rapid quality control of incoming materials using the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=Cary+630+FTIR">Agilent Cary 630 FTIR&nbsp;</a><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_battery_electrode_binder_cary_630_ftir_5994_8465en_agilent_4edc49f78f.pdf">Full PDF for download</a></li></ul>Lithium-ion battery (LIB) production relies on a wide variety of specialized materials sourced from numerous suppliers. In high-throughput LIB manufacturing facilities, even minor inconsistencies in raw materials can lead to significant downstream effects on cell performance. Binders are a good example: although used in small quantities to adhere active materials to metal current collectors, they are critical to electrode functionality. Binders directly influence electrode mechanical strength, uniformity, and long-term cell performance. As a result, production engineers and quality control (QC) managers consider binder verification as a key part of quality assurance (QA) workflows.&nbsp;Traditional binders such as polyvinylidene fluoride (PVDF) are effective but introduce environmental and solvent-handling concerns. Alternatives such as polytetrafluoroethylene (PTFE), styrene-butadiene rubber (SBR), and carboxymethyl cellulose (CMC) are increasingly being adopted within the industry to align with evolving sustainability standards. However, these changes also introduce new risks for LIB manufacturers — particularly when material specifications vary or supply sources change. To maintain production consistency and reduce the risk of batch failure or rework, fast and accurate material identification and verification tools are needed. FTIR spectroscopy offers a simple yet powerful solution for the QA/QC of various LIB raw materials. Given the organic and polymeric nature of binders, FTIR spectroscopy offers a rapid and reliable means of identifying them, as discussed in the "Characterization of PVDF polymorphic phases" section of this application note.&nbsp;This study outlines how the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=Cary+630+FTIR">Agilent Cary 630 FTIR spectrometer</a> (Figure 1) with its compact footprint can be used at the point of material receipt to verify binder identity. User-friendly Agilent MicroLab software ensures successful operation following minimal operator training. Analyses carried out using the Cary 630 FTIR support fast decision‑making, help avoid production disruptions, and improve end-product reliability.<h4>Results and discussion&nbsp;</h4>The binder samples were analyzed by placing a small quantity of solid material directly onto the ATR crystal of the Cary 630 FTIR spectrometer. Gentle pressure was applied to ensure good contact. After the measurement was completed, the ATR crystal was cleaned with a light solvent and wipe in preparation for the next sample.&nbsp;The MicroLab software uses a built-in Similarity algorithm that automatically calculates a Hit Quality Index (HQI). The HQI value indicates how closely the acquired sample spectrum matches a reference spectrum from the user‑generated library. HQI values are commonly used as pass/fail criteria in material identification and confirmation&nbsp;workflows.&nbsp;Using the Similarity algorithm, all four unknown samples (samples 1 to 4) were correctly identified according to the binder type stated on the respective container labels. However, variations in HQI values were observed among the PVDF samples (1 to 3). As shown in Table 2, PVDF-210 and PVDF-5130 exhibited HQI values of 0.96271 and 0.96713, respectively, indicating strong spectral matches. PVDF-6020 showed a slightly lower HQI of 0.90741, which may reflect differences in polymer grade or formulation compared to the reference sample. PTFE-104 (Sample 4) was also correctly identified, with a high HQI of 0.98345, suggesting good spectral agreement and a high-confidence match.<h4>Conclusion&nbsp;</h4>An Agilent Cary 630 FTIR spectrometer with Agilent MicroLab software provided a simple solution for identifying electrode binder materials—a typical application in lithium-ion battery quality control (QC) workflows.&nbsp;The MicroLab software enabled the rapid creation of a spectral reference library for binder materials based on the analysis of two known "reference" samples. Four "unknown" binders from a different supplier were then correctly identified using the FTIR method. However, one sample yielded a low, red color-coded confidence score, suggesting possible differences in polymer grade or formulation between the reference and unknown samples.&nbsp;The Cary 630 FTIR enables production and QC teams to:&nbsp;<ul><li>Adapt quickly to new materials or suppliers through easy management of the spectral library&nbsp;</li><li>Verify the identity of incoming raw materials using intuitive pictorial guidance and color-coded pass/fail results&nbsp;</li><li>Detect any mislabeling or contamination of materials&nbsp;</li><li>Monitor batch-to-batch variability that could affect electrode performance and battery manufacturing yields&nbsp;</li></ul>With its compact, modular design, the Cary 630 FTIR can be deployed in production environments, including gloveboxes. Its ease-of-use makes it a valuable tool for ensuring material consistency, minimizing process disruptions, and maintaining high standards in lithium-ion battery manufacturing.<h3>2. Shimadzu / AOAC: Speciation Analysis of Mercury in Seafood by LC-ICP-MS</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/aoac_2025_m028_b60b5ecaa6.pdf">Full PDF for download</a></li></ul>Mercury contamination in seafood poses significant health risks to consumers. Mercury screening using ICPMS alone can provide a total concentration, however some species of mercury are more toxic to humans than others, and a total concentration may not provide adequate information and result in incomplete risk assessments. Generally, methyl mercury is more toxic than inorganic mercury and other organic mercury. Evaluating the toxicity in food requires not only analysis as total mercury but also by the form of mercury. To address this, we propose the use of Liquid Chromatography coupled with Inductively Coupled Plasma Mass Spectrometry (LCICP-MS) for detailed speciation analysis of mercury by connecting an <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=ICPMS-2040%2F2050">ICPMS-2040/2050 </a>with <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+XS+inert">Nexera XS inert.</a> Furthermore, we also evaluated the automatic dilution function of the Nexera series autosampler for the speciation analysis of mercury.<h4>Results&nbsp;</h4>Samples were analyzed with the LC-ICP-MS system, which consisted of the ICPMS-2040/2050 connected to a Nexera XS inert (Fig. 1). LabSolutions ICPMS TRM software can control the ICPMS2040/2050 system and Shimadzu LC units, enabling everything from sample injection to chromatogram analysis to be performed via a single software program. The analytical conditions used for analysis were those included in the LC-ICP-MS Method Package for Mercury Speciation Analysis. Table 1 shows the analytical conditions for HPLC, and Table 2 shows the analytical conditions for ICP-MS.<h4>Conclusion</h4>Speciation analysis of methyl mercury and total mercury in seafood was performed using an LC-ICP-MS system that connected an ICPMS-2040/2050 to a Nexera XS inert according to the conditions in the “LC-ICP-MS Method Package for Mercury Speciation Analysis.” Analysis of methyl mercury and total mercury in seafood showed good spike recoveries demonstrating system suitability.<h3>3. Thermo Fisher Scientific: High Sensitivity, Fast Scanning, Sector Field ICP-MS – Improving Sensitivity for Laser Ablation with the Jet Interface</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_65415_icp_ms_high_sensitivity_fast_scanning_ewcps2019_po65415_en_22e55fbf12.pdf">Full PDF for download</a></li></ul>In laser ablation ICP-MS analysis, the choice of which type of ICP-MS to use may not be entirely obvious. High precision isotope ratio analysis, such as epsilon level precision for geochronology, would need to be measured with a multicollector ICP-MS, such as the Thermo Scientific™ NEPTUNE XT™, but for the majority of applications the precision obtainable with a single collector ICP-MS is sufficient. In the area of single collector ICP-MS we are still left with the choice between a quadrupole-based ICP-MS, such as the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1206">Thermo Scientific™ iCAP™ RQ/TQ</a>, and a magnet sector based ICP-MS, the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1670">Thermo Scientific™ Element XR™</a>.&nbsp;The reduced cost, size and complexity of the quadrupolebased ICP-MS make them a popular choice for LA-ICP-MS analysis. However, it has always been known that a magnet sector based ICP-MS enjoys a significant increase in sensitivity: detecting more ions for the same amount of atoms introduced. In laser ablation analysis the amount of sample to be analyzed is often strictly limited. Furthermore, to reduce laser-induced fractionation and to stay within discrete sample zones it is highly desirable to ablate as little sample material as possible. The trend towards higher resolution LA-ICP-MS bioimaging has also led to a reduction in the amount of sample ablated. These limitations in sample size make the sensitivity of the ICP-MS vital in order to achieve useable limits of quantification and detection (LOQ and LOD).The Jet Interface option for the Thermo Scientific Element XR HR-ICP-MS greatly increases the sensitivity of fast scanning, sector field ICP-MS, especially for dry plasma. It consists of a high capacity dry interface pump and a companion set of specially designed cones. Here we report the sensitivity (as sample ion yield) of the Element XR when equipped with the Jet Interface option. We then compare this sensitivity to two other ICP-MS for the LA-ICP-MS U-Pb analysis of two common zircon reference materials.&nbsp;<h4>MATERIALS AND METHODS&nbsp;</h4><h5>ICP-MS&nbsp;</h5>Three ICP-MS systems were used:&nbsp;<ul><li>NEPTUNE XT (multi collector ICP-MS, with Jet Interface)&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1670">ELEMENT XR</a> (single collector Sector Field ICP-MS), equipped with Jet Interface option&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/1206">iCAP TQ</a> (single collector quadrupole based ICP-MS), equipped with high sensitivity cones and insert recommended for laser ablation analysis</li></ul><h4>CONCLUSIONS&nbsp;</h4>The Jet Interface for the Element XR SF-ICP-MS gives:&nbsp;<ul><li>Sample Ion Yield &gt;1% for Nd, Hf, Pb and U.&nbsp;</li><li>Over 20 times LA sensitivity compared to Q-ICP-MS</li></ul>

News from LabRulezICPMS Library - Week 40, 2025

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th October 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you other document by Agilent Technologies and posters by Shimadzu / AOAC, Thermo Fisher Scientific / ASMS and Waters Corporation / AOAC!<h3>1. Agilent Technologies: PFAS Food Legislation Overview</h3><ul><li>Other document</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ebook_pfas_food_legislation_5994_8575en_agilent_dfe5a6222c.pdf">Full PDF for download</a></li></ul>This Agilent Technologies eBook provides an overview of global PFAS (per- and polyfluoroalkyl substances) regulations and analytical methods for monitoring these compounds in food, beverages, and food-contact materials. PFAS are synthetic chemicals valued for their water- and grease-resistant properties but are highly persistent and bioaccumulative. Their potential health risks—including immune suppression, elevated cholesterol, liver enzyme changes, and developmental effects—have prompted worldwide regulatory action to limit exposure through food and packaging.In the European Union, Regulation (EU) 2023/915 establishes maximum allowable levels of PFAS in various food products (e.g., meat, fish, eggs), ranging from 0.2 to 8 µg/kg depending on the matrix. Recommendation (EU) 2022/1431 encourages member states to collect PFAS occurrence data and harmonize testing methods, while the upcoming Packaging and Packaging Waste Regulation (PPWR 2025/40) will, from August 2026, limit PFAS in food packaging to 25 ppb per compound and 250 ppb total. Analytical testing across Europe relies primarily on liquid chromatography coupled with tandem mass spectrometry (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=32&amp;mainauthorItems=10">LC/MS/MS</a>), which provides the ppb-to-ppt sensitivity needed for regulatory compliance.In the United States, the framework combines federal, state, and industry standards. The USDA CLG-PFAS 2.04 method employs UHPLC/MS/MS to detect 16 PFAS in animal tissues, while the FDA C-010.03 method quantifies 30 PFAS in food and feed using solid-phase extraction (SPE) followed by LC/MS/MS. The AOAC SMPR 2023.003 standard defines performance criteria for PFAS testing across multiple food categories, ensuring validated and reproducible results. Several U.S. states—including Maine, California, and Washington—have also enacted specific limits for PFAS in meat, milk, and food-contact packaging.In China, national standard GB 5009.253-2016 specifies the determination of PFOS and PFOA in animal-derived foods via isotope-dilution LC/MS/MS. A new draft revision expands testing to 30 PFAS compounds (including isomers) across broader food matrices such as fish, vegetables, and milk. Chinese authorities have also introduced bans and restrictions on PFOS, PFOA, and PFHxS production and use, along with environmental management and disposal requirements.Agilent offers comprehensive PFAS testing solutions for food laboratories, including robust HPLC and LC/MS instrumentation, validated methodologies aligned with EPA, EU, and ASTM standards, and a curated PFAS MRM database. The portfolio also features Captiva EMR PFAS Food cartridges pre-tested for background contamination, certified PFAS standards, and data-handling tools such as Agilent MassHunter and SLIMS. Together, these workflows enable reliable PFAS quantification, reduced contamination risk, and full compliance with international food-safety legislation.<h3>2. Shimadzu / AOAC: Reliable and direct quantitative analysis of multi-mycotoxins in animal feed by using Shimadzu LCMS-8050RX</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/aoac_2025_m040_3bceabefcd.pdf">Full PDF for download</a></li></ul>Mycotoxins are toxic compounds produced by moulds that contaminate crops such as cereals, nuts, spices, dried fruits, apples, and coffee especially under warm, humid conditions. These chemically stable toxins can persist through food processing and pose serious health risks to humans and animals, ranging from acute poisoning to cancer and immunosuppressive. Among the many identified toxic compounds, Aflatoxins, Ochratoxin A, Fumonisins, T-2, Zearalenone, and Deoxynivalenol (DON) (Figure 1) are of particular concern. These are the most studied and regulated due to their significant impact on human and animal health, making their testing critically important. While conventional testing methods (HPLC-UV/FLD) are still common, regulatory focus is shifting toward LC-MS/MS testing due to its sensitivity and specificity.The European Committee for Standardization (CEN), under the M/520 mandate, is developing standardized methods for this purpose. Mycotoxins are generally extracted via liquid–liquid (for milk, wine, juices) or solid–liquid extraction (for cereals, dried fruits, spices, feed), typically using acidic buffers. In this study, we developed a fast, highthroughput LC-MS/MS method for multi-mycotoxin detection in animal feed using the Shimadzu LCMS8050RX (Figure 2). MRM based quantitative method was developed using Shim-pack GIST C18-AQ HPLC column with an aim to achieve improved peak separation and required sensitivities for the compounds under study. A matrix-matched calibration curve (0.5–50.0 ppb) showed accuracy between 80–120 %, and recoveries (at 2.5 and 5.0 ppb) were 70–120 %, complying with SANTE guidelines[1] .<h4>Materials and method</h4><h5>LC-MS/MS analysis</h5><ul><li>UHPLC: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+X3">Nexera X3</a></li><li>MS: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8050RX">LCMS-8050RX</a></li></ul><h4>Conclusion&nbsp;</h4><ul><li>Average recovery values for multi-mycotoxins in animal feed sample were found to be within the acceptance criteria as per the SANTE/12682/2019 guidelines.&nbsp;</li><li>The results obtained at 2.5 ppb and 5.0 ppb concentration level were accurate, repeatable and reproducible with RSD less than 20 %.</li><li>A simple liquid-liquid extraction method has been successfully developed and validated for the simultaneous quantification of nine mycotoxins in a single run.</li></ul><h3>3. Thermo Fisher Scientific / ASMS: High-throughput proteomics using narrow window DIA on the Orbitrap Astral Zoom MS</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_407_narrow_window_dia_proteomics_po407_asms2025_en_eea95fbc58.pdf">Full PDF for download</a></li></ul>The recently introduced Orbitrap Astral mass spectrometer has significantly expanded the scale and scope of proteomics experiments, advancing discovery and translational research. Building on this innovation, we demonstrate the performance of the next evolution of the platform, the Orbitrap Astral Zoom mass spectrometer, at throughputs ranging from 30 to 300 samples-per-day (SPD) analysing a HeLa digest using narrow window data-independent (nDIA) acquisition. The novel instrument features a higher acquisition rate (270 Hz vs. 200 Hz) achieved through improved ion optics settling times and faster ion transfers, increased sensitivity via pre-accumulation, and deeper coverage through enhanced spectral processing, among other improvements. Comparative analysis with the Orbitrap Astral MS reveals further improvements in peptide and protein group identifications enabling even deeper coverage at high throughput whilst maintaining precise quantitation.<h4>Methods</h4>HeLa digest standard measured on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a> and Thermo Scientific <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/836">Orbitrap Astral MS</a> coupled to Thermo Scientific <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC</a> utilizing 30-300 samples-per-day (SPD) LC-MS methods<h4>Conclusions&nbsp;</h4>The novel Orbitrap Astral Zoom mass spectrometer is, among its broad and comprehensive range of applications, an excellent instrument for high-throughput proteomics.&nbsp;<ul><li>High-throughput nDIA on the Orbitrap Astral Zoom MS generates deep coverage of proteomes</li><li>&nbsp;The novel Orbitrap Astral Zoom MS further pushes the boundaries in terms of quantity and quality of identifications&nbsp;</li><li>The Orbitrap Astral Zoom MS enables precise quantitation with full flexibility to focus on MS2-based quantitation</li></ul><h3>4. Waters Corporation / AOAC: Integration of Ultra-short Chain PFAS Into Routine Analysis Methods: Addressing Retention and Confirmatory Ions</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009019_f2e0c3e56f.pdf">Full PDF for download</a></li></ul>The poster presents a novel LC-MS/MS workflow enabling simultaneous quantification and confirmation of ultra-short through long-chain PFAS in a single analysis. The work was conducted using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/191">Waters ACQUITY Premier LC system</a> equipped with a PFAS kit, Atlantis Premier BEH C18 AX mixed-mode column, and a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/554">Xevo TQ Absolute tandem mass spectrometer</a> with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1544">waters_connect software</a> for quantitation.The study addresses two analytical challenges: (1) the poor chromatographic retention of ultra-short chain PFAS (e.g., TFA, PFPrA) on conventional reverse-phase columns and (2) the absence of confirmatory ions for some short-chain PFAS, which typically produce limited fragment ions. Using the Atlantis Premier BEH C18 AX column, which combines reversed-phase and anion-exchange mechanisms, the authors achieved stable retention of C2–C4 PFAS. Optimized pH control using ammonium hydroxide and acetic acid further stabilized retention times of highly polar compounds.For mass spectrometric confirmation, the team demonstrated the use of the m/z 19 fragment transition as a new confirmatory ion for PFAS that previously lacked one. On the Xevo TQ Absolute system, low-mass transmission enhancement significantly increased sensitivity for this ion, allowing confirmatory detection of PFBA and PFPeA even at low (ppt-level) concentrations. Ion ratio stability across calibration curves met common method validation criteria (±30%), demonstrating robustness for routine use.The combined method enables the simultaneous analysis of ultra-short to long-chain PFAS in a single injection without requiring additional columns or HRMS verification. Results from landfill leachate and surface water samples confirmed the method’s capability to resolve co-eluting interferences and to reliably identify PFAS at environmentally relevant levels. This workflow offers an efficient and practical solution for laboratories transitioning to include ultra-short chain PFAS within standard monitoring methods.

News from LabRulezLCMS Library - Week 41, 2025

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 19th January 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by Agilent Technologies, Shimadzu, Waters Corporation and poster by Thermo Fisher Scientific!<h3>1. Agilent Technologies: Automated Bioprocess Monitoring with Agilent 1290 Infinity III Bio Online LC Solutions</h3>Process analytics of L-alanine production using genetically modified Vibrio natriegens<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_automated_bioprocess_monitoring_1290_infinity_bioonline_solutions_5994_8865en_agilent_9f33de6ddf.pdf">Full PDF for download</a></li></ul>Bioprocess engineering is a challenging field because of the constantly changing nature of bioprocess models which make them difficult to validate.1 Consequently, process control needs to be developed at various levels using strategies tailored to these specific requirements.1 The performance and effectiveness of the bioreactor are determined by key performance indicators such as product yield, titer, production rate, and quality of the product.1 These factors can be quickly influenced by changes in process variables and the raw materials used.1,2 The ability to react quickly to problems is therefore crucial for preserving ongoing production, a factor that can be particularly significant in bioprocesses due to the substantial cost and time investmented.1,2 Such challenges can be tackled by constantly monitoring the bioprocess, which requires the ability to analyze and collect data quickly. Therefore, monitoring is of the utmost importance.&nbsp;This application note describes the advantages of using an Agilent 1290 Infinity III Bio Online LC solution with Agilent Online LC Monitoring software. A bioreactor was interfaced with a Flownamics Seg-Flow S3 sampling system for aseptic withdrawal and an Agilent 1290 Infinity III Bio Online LC to make an hourly analysis.&nbsp;This is a fully automated approach for taking sterile samples from a bioreactor and preparing or diluting them. In addition, the 1290 Infinity III Bio Online LC solution displays data in near real time, which is a crucial element for effective bioprocess&nbsp;monitoring.<h4>Experimental&nbsp;</h4><h5>Equipment&nbsp;</h5>The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III+Bio">1290 Infinity III Bio </a>Online LC comprised the following&nbsp;modules:&nbsp;<ul><li>Agilent 1290 Infinity III Bio Flexible Pump (G7131A) with an InfinityLab Quick Change Bio inline filter assembly (part&nbsp;number 5067-1607), 2.1 mm id, 0.2 µm pore size&nbsp;</li><li>Agilent 1290 Infinity III Bio Online Sample Manager (G3167B) with Sample Thermostat (option #101) clustered with Agilent 1290 Infinity Valve Drive (G1170A), featuring a reactor valve pod (part number 5067-6680) and Online LC Monitoring Software&nbsp;</li><li>Agilent 1290 Infinity III Multicolumn Thermostat (G7116B) equipped with an Agilent Quick Connect Bio Heat Exchanger Std. (G7116-60071)&nbsp;</li><li>Agilent 1290 Infinity III Diode Array Detector (DAD) (G7117B), equipped with a Bio Max-Light Cartridge Cell (G4212-6008), 10 mm, 1 µL&nbsp;</li><li>Agilent 1260 Infinity High-Performance Degasser (G4225A) is connected between S1 (transport solvent) solvent line and Agilent 1290 Infinity III Bio Online Sample Manager (G3167B)&nbsp;</li></ul><h5>Software&nbsp;</h5><ul><li>Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=OpenLab+CDS">OpenLab CDS</a> version 2.8 or later versions&nbsp;</li><li>Agilent Online LC Monitoring Software version 1.3 or later&nbsp;versions&nbsp;</li><li>Flownamics FlowWeb control&nbsp;software</li></ul><h5>Instrumental configuration&nbsp;</h5>The 1290 Infinity III Bio Online LC solution was used with a Seg-Flow interface for automatic sampling and online analysis (see Figure 1). The Seg-Flow interface was used to withdraw samples from a bioreactor through a Flownamics F-Series 310 FISP probe with a ceramic membrane filter (0.2&nbsp;µm pore size) from a 5 L bioreactor. The drawn samples were sent through the built-in sample collection cup on the Seg‑Flow to the LC and the samples were retained into vials. The collected samples were then automatically derivatized by using an injector program and subsequently analyzed. A more detailed description of the instrumental configuration has been published.3<h4>Conclusion&nbsp;</h4>For online bioprocess monitoring, a fully automated setup consisting of a bioreactor, a Flownamics sampling system, and an Agilent 1290 Infinity III Bio Online LC solution was successfully established. This setup is capable of taking aseptic samples from a bioreactor, preparing, analyzing, and processing samples without the need for manual interaction. In addition, the added value of the Agilent Online LC Monitoring software was evident in the display method and the ability to track it quickly.<h3>2. Shimadzu: Analysis of Oligonucleotide Impurities Using Single Quadrupole Mass Spectrometer</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00974_en_c50bb67c7a.pdf">Full PDF for download</a></li></ul><h4>User Benefits</h4><ul><li>Combination use of LCMS-2050 single quadrupole mass spectrometer and LabSolutions Insight Biologics provides comprehensive characterization of oligonucleotides and related impurities.</li><li>Purity calculations for target components can be performed even on poorly separated peaks by utilizing MS spectra simultaneously acquired with UV chromatogram.</li></ul>In recent years, oligonucleotide therapeutics have been paid attention as a novel drug discovery modality because of their rapid progress of development. During the synthesis of oligonucleotide therapeutics, impurities that have differing base chain lengths are generated along with the target oligonucleotide. To ensure the safety and efficacy of the drug, comprehensive detection and identification of these impurities are required.&nbsp;Reversed phase ion-pair chromatography is often employed as a method to separate the target compound from co-existing impurities. However, it is often difficult to establish analytical conditions that provide compete separation of all impurities that are formed in synthesis process due to their similar structures and properties to the principal compound of active ingredient. Against this drawback, liquid chromatography-mass spectrometry (LC-MS) is commonly used for impurity detection and identification. For analysis of oligonucleotide impurities, quadrupole time-of-flight mass spectrometers are mainly used because of their high mass resolution and ability to perform sequence and structure estimations via MS/MS analysis. However, in quality control process, the use of a single quadrupole mass spectrometer is expected to be advantageous because of providing mass information with easy operation when impurity information is already known.&nbsp;This article presents a simulated impurity analysis of synthetic oligonucleotide using the LCMS-2050 high-performance liquid chromatography-mass spectrometer and LabSolutions Insight Biologics.<h4>Analytical conditions</h4>The employed instrument setup was <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+XS">Nexera XS inert</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-2050">LCMS-2050</a>. HPLC conditions are shown in Table 2, and MS conditions are shown in Table 3. The conditions were set where n-1(5') co-eluted with FLP, while n-3(5') was separated (Fig. 1) to demonstrate impurity analysis performance.<h4>Conclusion</h4>The simulated sample consisting of mainly FLP and small amounts of n-1(5')/n-3(5') was analyzed using the LCMS-2050 and LabSolutions Insight Biologics software and obtained data were processed appropriately. Detection, identification, and quantitation at low concentrations were accomplished not only for n-3(5') well-separated from FLP but also for n-1(5') co-eluted with FLP. In case that establishing analytical conditions is difficult, Using this instrument setup and software will provide efficient quality confirmation of oligonucleotide therapeutics.<h3>3. Thermo Fisher Scientific: Simultaneous Quantitation and Untargeted Discovery (SQUAD) workflow using multiple fragmentation techniques for quantitation and characterization of trace level nitrosamine in biological matrices</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_squad_workflow_multiple_fragmentation_techniques_po_en_94a5b27b12.pdf">Full PDF for download</a></li></ul>N-Nitrosamines are a class of carcinogenic chemical compounds that pose threat to humans exogenously through matrices like drinking water, food, cosmetics, and endogenously by nitrosation in gastrointestinal tract, nitrate to nitrite conversion, etc. An accurate, reliable analytical method is critical for detection and quantification of these trace-level, polar and low molecular weight compounds to understand the association between their exposure and related diseases.&nbsp;An optimized SQUAD workflow is presented that discovers unknown compounds in the matrix by using high resolution orbitrap and a sensitive linear ion trap detector that quantifies the low intensity nitrosamines in a single run. The workflow uses multiple fragmentation types – HCD, CID and UVPD and MSn to help characterize isomeric structures.<h4>Methods</h4>Serial dilution of Nitrosamine standards were subjected to Simultaneous Quantitation and Discovery (SQUAD) analysis using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Thermo Scientific™ Vanquish™ Horizon UHPLC system</a> and the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/561">Thermo Scientific™ Orbitrap IQ-X™ Tribrid™ mass spectrometer</a> . Nitrosamine spiked matrices of human pooled urine and human Serum were subjected to SQUAD analysis.<h4>Data Analysis&nbsp;</h4>Spectra were evaluated for nitrosamines using Thermo Scientific™ FreeStyle™ software and annotated using Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757">Mass Frontier™ 8.0 software</a>. Untargeted data analysis was performed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Compound Discoverer 3.4 software</a>. Calibration curves were generated using Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77">TraceFinder™ 5.2 software</a>.<h4>Conclusions&nbsp;</h4><ul><li>Detection limits as low ppb levels ( 0.2 ppb –0.5 ppb) and quantitation limits (0.2 ppb- 5 ppb ) in presence of the matrix were achieved for all seven nitrosamines by applying parallel ion trap analysis&nbsp;</li><li>SQUAD analysis was run on Orbitrap IQ-X Tribrid MS and achieved low ppb level quantitation in ion trap and identified unknown compounds through discovery analysis in Orbitrap.&nbsp;</li><li>Employing nano LC helped achieve lower limit of quantitation (5 ppt)</li></ul><h3>4. Waters Corporation: Validated LC-MS/MS Method for Vitamin E Acetate in Cannabis Plants and Derived Products</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009082_en_c7a6fc6190.pdf">Full PDF for download</a></li></ul><h5>Benefits&nbsp;</h5><ul><li>The validated LC-MS/MS method provides high sensitivity and specificity for VEA quantification in complex cannabis matrices, including flower and concentrates.&nbsp;</li><li>Minimal sample preparation reduces handling time and improves laboratory throughput, supporting efficient routine analysis.&nbsp;</li><li>The method demonstrates robust performance across multiple matrices, ensuring reliable detection even in lipid-rich, chemically diverse samples.</li><li>Use of Waters ACQUITY UPLC H-Class System and Xevo TQ-S micro Mass Spectrometer enables accurate quantitation well below regulatory requirements.&nbsp;</li><li>The validated method complies with Missouri DCR and DHSS regulatory limits, enabling laboratories to confidently monitor VEA in cannabis products.</li></ul>VEA is used as a diluent and viscosity modifier in certain cannabis-derived products, including vaping formulations. Following increased public health awareness during 2019, VEA became a marker of interest for quality control and regulatory surveillance across cannabis supply chains due to its potential impact on lung health. In addition to concentrates and vape liquids, trace residues may be present in infused products and processing intermediates. Therefore, reliable quantification of VEA in these chemically diverse, lipid-rich matrices requires an analytical method that is sensitive, selective, and robust against matrix effects. In February 2024, Missouri’s DCR revised the tolerance limit for VEA in cannabis products from 0.2 ppm to 5 ppm.1&nbsp;Liquid chromatography coupled with tandem quadrupole mass spectrometry (LC-MS/MS) provides the sensitivity and specificity required to distinguish VEA from endogenous cannabinoids, terpenes, and other lipophilic excipients, while also offering the sensitivity to support low-ppm and sub-ppm action limits. Unlike UVbased methods, where co-eluting chromophores may interfere, LC-MS/MS enables targeted multiple reaction monitoring (MRM) for confident identification and accurate quantitation in complex cannabis matrices.&nbsp;This application note describes the validation of an LC-MS/MS method for the quantitation of VEA in two inhalable products, cannabis flower and cannabis concentrates. The method was developed and validated using the Waters ACQUITY UPLC H-Class System coupled with a Xevo TQ-S micro Triple Quadrupole Mass Spectrometer. Chromatographic separation was achieved with a reversed-phase ACQUITY UPLC BEH C18 Column. A streamlined sample preparation approach was applied for cannabis flowers and concentrates, which minimizes handling time and increases sample throughput.&nbsp;The validation study was conducted at GPA. Method performance was evaluated for calibration range, linearity, LOD, LOQ, accuracy, precision, recovery, repeatability, and robustness. The results of these are evaluated against acceptance criteria set by State of Missouri DHSS and presented in this application note.<h4>Experimental&nbsp;</h4><ul><li>LC system: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198">ACQUITY UPLC H-Class Plus System&nbsp;</a></li><li>MS detection: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/209">Xevo TQ-S micro Mass Spectrometer&nbsp;</a></li><li>Column: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1952">ACQUITY UPLC BEH C18 Column</a>, 2.1 x 50 mm, 1.7 µm (p/n: 186002350)</li><li>Software: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/635">MassLynx™ 4.1 Software&nbsp;</a></li><li>Data processing: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=TargetLynx">TargetLynx™</a> Application</li></ul><h4>Conclusion&nbsp;</h4>The LC-MS/MS method developed and validated for VEA quantification in cannabis flower and concentrates demonstrates excellent linearity, accuracy, precision, and robustness, and meets the regulatory requirements. Calibration curves achieved R² values greater than 0.999, with LOD and LOQ well below the Missouri action limit of 5 ppm. Matrix-spiked recoveries across flower and concentrate samples were within the 70–130% acceptance range, and QC samples met all performance criteria, confirming method reliability. Robustness testing across analysts and matrices further validated the method’s reproducibility. Overall, this approach provides a streamlined, sensitive, and regulatory-compliant workflow for laboratories, enabling accurate monitoring of VEA to support quality control, consumer safety, and regulatory compliance in cannabis-derived products

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