Although 100% recovery is expected in SPE, values above 100% are sometimes observed. This article explains the most common causes and provides practical guidance on how to identify and resolve them.

A technical guide to troubleshooting low analyte recovery in SPE. Explains relative vs. absolute recovery and provides practical solutions for conditioning, loading, and sorbent selection.

Phospholipids in serum and plasma can cause ion suppression, reduced MS sensitivity, and shorter column lifetime. Learn why phospholipid removal is critical for reliable LC-MS analysis.

How can neutral analytes be efficiently concentrated in the presence of ionized acidic or basic contaminants? This SPE tip explains how mixed-mode cartridges improve selectivity and cleanup.

Practical guide to oligonucleotide sample preparation using SPE. Learn how to optimize wash and elution steps for clean extracts and reliable LC-MS bioanalysis.

A streamlined workflow for N-linked glycan sample preparation, combining PNGase F digestion, fluorescent labeling, and HILIC SPE clean-up for reliable HPLC and LC-MS analysis.

Matrix effects can suppress or enhance analyte signals and compromise accuracy. Learn how sample prep techniques—especially SPE with Strata-X PRO—reduce phospholipid interference and improve LC-MS results.

A clear guide to Normal Phase SPE: when to use it, how polar interactions drive retention, and which solvents to choose in increasing polarity—plus a quick reference list for optimized sample preparation.

Learn how to evaluate column loadability when scaling from analytical to preparative HPLC, and how to balance purity, yield, and productivity for optimal purification.

GC performance often declines due to worn or contaminated inlet parts. Regular replacement of liners, septa, and key components reduces failures, prevents downtime, and keeps results consistent.