<h3>What is Samplify?</h3>Samplify is an innovative new technology from SIELC, specifically designed for unattended, routine, periodic sampling of virtually any liquid source. Each sample is transferred automatically into an individual destination vial or 96-well plate. Vial trays or plates may then be loaded directly into a variety of analytical systems for further processing.<blockquote>Precise, Reproducible, Unattended Sampling – from: Reactors, Fermenters, Dissolution Vessels, Process Streams, and other Liquid Sources.</blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_2_e6db88c004.jpg" alt="Bioanalytic: Samplify from SIELC Technologies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_2_e6db88c004.jpg 127w," sizes="100vw" width="1012" height="1247">The Samplify system consists of two principal components:<ol><li>&nbsp;a sophisticated electro-mechanical sample probe constructed of materials providing chemical and temperature resistance, and</li><li>SIELC’s renowned and highly flexible liquid handler (LHS) for pulling samples from the liquid source and distributing samples into destination locations.</li></ol>The LHS offers many additional capabilities; for example, the addition of quenching reagents into sample vials immediately after acquisition, if required, and thorough probe cleaning to guard against cross-sample contamination. The entire system may be controlled with SIELC’s Samplify Windows app, or via serial port using provided commands.<blockquote>Samplify is ultra-compact, allowing placement of the probe – or the probe plus LHS, as required – in confined spaces such as a reaction hood or a refrigerator.</blockquote><h3>Samplify Specifications</h3><h4>Samplify Probe</h4><ul><li>Connection: requires Samplify LHS</li><li>Small dimensions: 11 x 1 inch, DIA 1/4 inch. Immersion length up to 6”</li><li>Contact with liquid: Stainless Steel 316 (316L), PEEK &amp; PTFE</li><li>Variable sample volume: from 5 to 500 μL</li><li>Max. working pressure: 120 psi (8 bar)</li><li>Max. working temperature: 200 °C</li></ul><h4>Samplify LHS</h4><ul><li>Instrument Size: 6 x 6.5 x 7 inch (15 x 16 x 17 cm)</li><li>Weight: 5 lb (2.3 kg)</li><li>Collection Options: 48 vial plate (2 mL vials); 96 well plate</li><li>Pressure Max: 250 psi/ 14 bar (4000 μL volume)</li><li>Syringe Capacity Options: 4000 μL</li><li>Valve Options: 7x6</li><li>Volume Accuracy: +/-1 μL (4000 μL volume)</li><li>Probe dead volume: 767 μL</li><li>Syringe draw/refill rates: 48,000 μL/min</li><li>Communication: USB (type B) COM port</li><li>Power: 24 V DC</li><li>Contact with liquid: Stainless Steel 316 (316L), PEEK, PTFE, Vespel</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_Probe_3c4d643d62.jpg" alt="Bioanalytic: Samplify from SIELC Technologies - Probe." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_Probe_3c4d643d62.jpg 108w," sizes="100vw" width="1299" height="1867"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_LHS_e16e757cd9.jpg" alt="Bioanalytic: Samplify from SIELC Technologies - LHS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sampling_Solutions_Start_Here_Samplify_from_SIELC_Technologies_Samplify_TM_LHS_e16e757cd9.jpg 168w," sizes="100vw" width="859" height="799">

Sampling Solutions Start Here: Samplify from SIELC Technologies

<blockquote>Rafał Szewczyk1,2, Anna Lenartowicz1, Alina Kuryłowicz3, Katarzyna Krupczyńska-Stopa1,2, Maciej Stopa1,21. LabExperts sp. z o. o., Gdańsk, Poland; 2. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315">Bioanalytic</a> sp. z o. o., Gdańsk, Poland; 3 Department of Human Epigenetics, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland</blockquote><h4>Introduction</h4>Obesity is a growing problem that affects more than 1 billion people worldwide. Estrogens deficiency play an important role in the pathogenesis of obesity. Therefore, there is a need to characterize sex hormones profile in adipose tissues in normal-weight and obese individuals to predict the effectiveness of estrogen-oriented approach in prevention and treatment of obesity. Determination of steroid hormones in human adipose tissue is challenging due to the low concentration of hormones, low amount and complexity of matrix. LC-MS/MS is an attractive analytical technique for development of such challenging methods because of high specificity, sensitivity and short analysis time. In this work we present LC-MS/MS method for a quantitative determination of estradiol, estrone, androstenedione and testosterone in human adipose tissue.<h4>Methods</h4>Sample preparation method consists of two stages: 1) liquid–liquid extraction of previously homogenized and frozen tissue using ethanol:ethyl acetate (1:1) mixture with internal standards addition. Upper layer obtained after this extraction was collected and evaporated under nitrogen stream and reconstituted in 20% MetOH; 2) Solid-phase extraction (SPE) using Sep-Pak® C18 Cartridges; 2g sorbent (Waters). Elution fraction collected after SPE procedure was evaporated under nitrogen stream and reconstituted in 40% MetOH. Compounds were analyzed using reversed-phase chromatography on Nexera LC40 (Shimadzu) LC coupled with QTRAP 6500+ (SCIEX) operating in positive and negative electrospray ionization scheduled MRM mode. Analyte presence confirmation was based on 2 criteria: specific retention time and ion ratio. Data were processed in SCIEX OS software.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Figure_1_Table_1_a673d66465.jpg" alt="Bioanalytic: Figure 1. Schematic representation of the sample preparation procedure." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Figure_1_Table_1_a673d66465.jpg 118w," sizes="100vw" width="873" height="1150"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Table_1_LC_MS_MS_method_parameters_31a6b1493d.jpg" alt="Bioanalytic: Table 1. LC-MS/MS method parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Table_1_LC_MS_MS_method_parameters_31a6b1493d.jpg 245w," sizes="100vw" width="2047" height="891"><h4>Preliminary data</h4>Literature data regarding methods determining both estrogens and androgens in human adipose tissue are limited. Because of different physicochemical properties of these two groups of steroid hormones it was considered to prepare two distinct methods in the optimization stage. Finally, after several different approaches tested, it was possible to develop single sample preparation and analytical method suitable for all the tested analytes with sufficiently good sensitivity. The method was validated separately for visceral (VAT) and subcutaneous (SAT) adipose tissue and fulfilled the following criteria for each analyte: linearity (R ≥ 0.995), reproducibility (%CV ≤ 15%), accuracy in the range: 80 – 120%. Method’s working range depends on adipose tissue type and analyte (visceral: androstenedione 1-100 ng/g; testosterone 0.1-10 ng/g; estradiol and estrone 0.05-10 ng/g; subcutaneous: androstenedione 1-100 ng/g; testosterone, estradiol and estrone 0.1-10 ng/g). Developed method was successfully applied to analyze adipose tissue samples collected from normal-weight and obese patients using only 0.25 g of homogenized tissue sample, what is important in case of limited clinical material availability.&nbsp; The analysis was performed on pairs of visceral and subcutaneous adipose tissue from 15 obese patients (10 women and five men) and five pairs of adipose tissues from normal weight subjects (40 tissues in total). It was found that the concentrations of androstenedione, testosterone and estrone were higher while estradiol lower in visceral tissues than in subcutaneous tissues, irrespective of body weight. The tissues of obese patients (both VAT and SAT) were characterized by higher concentrations of estrone, while normal weight subjects had significantly higher testosterone concentrations in visceral than in subcutaneous adipose tissue depot.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Figure_3_b8063adb06.jpg" alt="Bioanalytic: Figure 3. Chromatogram of LC-MS/MS analysis of extracted tissue sample with marked ionization parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Figure_3_b8063adb06.jpg 245w," sizes="100vw" width="1874" height="1155"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Table_2_2198fdaeed.jpg" alt="Bioanalytic: Table 2. Summary of method validation results depending on the adipose tissue type." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Table_2_2198fdaeed.jpg 245w," sizes="100vw" width="3122" height="886"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Figure_4_fb31f04031.jpg" alt="Bioanalytic: Figure 4. Charts representing results of analysis of adipose tissue samples collected from normal-weight and obese patients. VAT-O - visceral tissue from obese patients; SAT-O subcutaneous tissue from obese patients; VAT-N - visceral tissue from normal-weight patient; SAT-N - subcutaneous tissue from normal weight patient." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Sensitive_quantitative_LC_MS_MS_method_of_steroid_hormones_determination_in_adipose_tissue_as_a_helpful_tool_in_obesity_research_Figure_4_fb31f04031.jpg 220w," sizes="100vw" width="1756" height="1247"><h4>Novel aspect</h4>Sensitive LC-MS/MS method allowing for simultaneous quantitation of estrogens and androgens in single run using low amount of adipose tissue.

Sensitive quantitative LC-MS/MS method of steroid hormones determination in adipose tissue as a helpful tool in obesity research

<blockquote>Julia Mironenka1, Rafał Szewczyk1,2, Katarzyna Krupczyńska-Stopa1,2, Maciej Stopa 1,2&nbsp;LabExperts sp. z o. o., Gdańsk, Poland; 2. Bioanalytic sp. z o. o., Gdańsk, Poland;</blockquote><h4>Introduction</h4>Antibiotics are commonly used in therapy of infectious diseases. Their overuse to increase animal productivity, including honey production, has become a serious problem in food safety regulations. High doses of antibiotics used by farms, find their way into the end products that enter human consumption. Honey is a valuable product, often used in medicine and as a dietary ingredient. All the sweetness of honey disappears when it reaches the laboratory as a matrix for LC-MS/MS analysis, due to the great diversity of components content between each, or even the same kind of honey. In this work we have focused on the development of highly reproducible and precise method of 57 antibiotics determination by LC-MS/MS in honey, regardless of its origin.<h4>Methods</h4>Sample preparation is based on modified QuEChERS method. The honey samples after enrichment with known concentrations of analytes were hydrated using acidified water, heated and sonicated until sugar dissolved. Next, after mixing with acidified acetonitrile solution, samples were desalted using ready to use QuEChERS sachets and filtered with PVDF syringe filter. &nbsp;Before the LC-MS/MS analysis samples were diluted with water. Data were acquired on QTRAP 5500+ (SCIEX) mass spectrometer coupled with ExionAC LC (SCIEX) and processed with SciexOS 2.2 software. The LC-MS/MS analysis was performed in positive ionization sMRM mode during reversed phase 12-min separation on Fortis H2O C18 chromatographic column. Mobile phases consisted of water, acetonitrile and formic acid additive.<figure class="image image_resized" style="width:50%;"><img style="aspect-ratio:362/1175;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_How_to_deal_with_sweet_matrix_a_method_for_LC_MS_MS_analysis_of_antibiotics_in_honey_regardless_of_its_kind_Fig_1_Sample_preparation_procedure_efcd25086b.jpg" alt="Bioanalytic: Fig 1. Sample preparation procedure." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_How_to_deal_with_sweet_matrix_a_method_for_LC_MS_MS_analysis_of_antibiotics_in_honey_regardless_of_its_kind_Fig_1_Sample_preparation_procedure_efcd25086b.jpg 48w," sizes="100vw" width="362" height="1175"></figure><h4>Preliminary data</h4>Honey is a complex matrix that consists of over 180 components. Depending on the origin, type and bees, the ingredients are present in different amounts, which undoubtedly affects its properties. Developing a repeatable extraction method for one compound becomes a challenge. In the present work a multimethod for 57 analytes from 10 different classes of antibiotics was developed. The optimization of the sample preparation method consisted of: selection of solvents and pH of the solutions, concentration of additives, time of sonication and extraction, dissolution ratio and method of cleaning the sample before LC-MS/MS analysis. The intensity and shape of signals of samples subjected to evaporation under nitrogen concentration and dissolved in the initial phase of the chromatographic method were compared to samples analyzed immediately after extraction with different water dilutions. The present method allows sample preparation without time-consuming stage of evaporation followed by residues dissolving.The efforts devoted to method development resulted in obtaining averaged recovery of tetracyclkines, cefalosporins, sulfonamides and fluorochinones was found to be 106.4%. The linearity (R&lt;0.995) covered wide concentration range 0.5-500 µg/kg. LLOQ for most macrolides (tylosin, spiramycin, tilmicosin) and tetracyclines (doxycycline, chlorotetracycline, 4-epi-chlorotetracycline) was 0.5 µg/kg, for some of beta-lactams (nafcillin, cloxacillin, oxacyllin) was 0.1 µg/kg and for some sulfonamides (sulfadimethoxine, sulfachinoxaline, sulfchloropyrazine) reached 0.05 µg/kg. Thanks to a laborious optimization process excellent repeatability for 10 different honeys obtained from local beekeepers was achieved. The accuracy and precision for the whole set of honeys was lower than 20% in the working range of the assay.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_How_to_deal_with_sweet_matrix_a_method_for_LC_MS_MS_analysis_of_antibiotics_in_honey_regardless_of_its_kind_Table_1_The_results_of_method_validation_6a3be83107.jpg" alt="Bioanalytic: Table 1. The results of method validation." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_How_to_deal_with_sweet_matrix_a_method_for_LC_MS_MS_analysis_of_antibiotics_in_honey_regardless_of_its_kind_Table_1_The_results_of_method_validation_6a3be83107.jpg 245w," sizes="100vw" width="2126" height="1166"><figure class="image"><img style="aspect-ratio:897/575;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_How_to_deal_with_sweet_matrix_a_method_for_LC_MS_MS_analysis_of_antibiotics_in_honey_regardless_of_its_kind_Table_2_Table_3_Table_4_58dd61a3ef.jpg" alt="Bioanalytic: Table 2. Ion source parameters; Table 3. sMRM scan parameters; Table 4. General LC-MS parameters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_How_to_deal_with_sweet_matrix_a_method_for_LC_MS_MS_analysis_of_antibiotics_in_honey_regardless_of_its_kind_Table_2_Table_3_Table_4_58dd61a3ef.jpg 244w," sizes="100vw" width="897" height="575"></figure><h4>Novel aspect</h4>The effective analytical procedure for the determination of 57 antibiotics residues from different honey samples in one LC-MS/MS run.

How to deal with sweet matrix? – a method for LC-MS/MS analysis of antibiotics in honey, regardless of its kind

<blockquote>Adrian Soboń (1,2); Julia Mironenka (1); Anna Lenartowicz (1); Rafał Szewczyk (1,2); Katarzyna Krupczyńska-Stopa (1,2); Maciej Stopa (1,2); Andrzej Kwaśnica (3)1 – LabExperts sp. z o. o., Gdańsk, Poland; 2 – Bioanalytic sp. z o. o., Gdańsk, Poland; 3 – Lab4Tox sp. z o. o., Wrocław, Poland</blockquote><h4>Introduction</h4>Anabolic androgenic steroids (AAS) are a group of natural and synthetic compounds that are chemically similar to and mimic the actions of endogenous testosterone. They stimulate androgen receptors, contributing to an increase in muscle mass and strength, and on the other hand reduction of body fat. Although the use of AAS in sports is banned, they are still the most abused class of drugs in sports. High-resolution mass spectrometry (HRMS) offers analytical laboratories a powerful tool to detect drugs and efficiently and confidently identify them using mass defect data on precursor and quantifier ion with parallel spectral library matching followed by quantitative analysis at low levels.<figure class="image"><img style="aspect-ratio:1452/1062;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_HR_MS_MS_EAD_and_CID_fragmentation_leads_to_selective_sensitive_and_reliable_quantitation_of_anabolic_steroids_in_urine_Figure_1_8a460d9979.jpg" alt="Bioanalytic: Figure 1. Workflow of sample pretreatment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_HR_MS_MS_EAD_and_CID_fragmentation_leads_to_selective_sensitive_and_reliable_quantitation_of_anabolic_steroids_in_urine_Figure_1_8a460d9979.jpg 214w," sizes="100vw" width="1452" height="1062"></figure><h4>Methods</h4>Sample preparation starts with enzymatic hydrolysis of potential anabolic conjugates, followed by the clean-up procedure using a mix-mode SPE cartridge (C8 + aminopropyl). AAS were analyzed using ZenoTOF 7600 system with the time scheduled Zeno MRMhr for optimal sensitivity using both collision-induced dissociation (CID) and electron-activated dissociation (EAD).Chromatographic separation was done on a Kinetex 2.6 µm XB-C18 (100 A; 100 x 2.1 mm) column using an ExionLC AC system (Sciex). Mobile phase solvents were water (A) and mix of methanol and acetonitrile (1:1 v/v) (B). Separation was performed at a flow rate of 500 µl/min with a column temperature of 40 °C. Data processing was made using SCIEX OS v.3.1 software.<figure class="image"><img style="aspect-ratio:1985/840;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_HR_MS_MS_EAD_and_CID_fragmentation_leads_to_selective_sensitive_and_reliable_quantitation_of_anabolic_steroids_in_urine_Table_2_Table_3_8f1bf5bce7.jpg" alt="Bioanalytic: Table 2. Compounf settings in MRM HR (CID) method. Table 3. Compounds settings MRM HR (EAD) method. Note, that it possible performed both in CID or EAD fragmentation in one method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_HR_MS_MS_EAD_and_CID_fragmentation_leads_to_selective_sensitive_and_reliable_quantitation_of_anabolic_steroids_in_urine_Table_2_Table_3_8f1bf5bce7.jpg 245w," sizes="100vw" width="1985" height="840"></figure><h4>Preliminary Data</h4>The gold standard in the LC-MS/MS quantitation is the scanning of two MRM pairs on low resolution (LR) triple quad instrument – quantifier and qualifier ion. Unfortunately, matrix effects may lead to insufficient confirmation of compound presence, especially at low levels where MRM ratio and matrix interferences can lead to result misinterpretation. To overcome the specificity problems in the LR data, HR system was used to establish sensitive, selective, qualitative and quantitative method to determine 28 AAS in urine matrix.Both collision-induced dissociation (CID) and electron-activated dissociation (EAD) fragmentation methods were used to select specific fragment ions for quantitation and MS/MS libraries for analytes. The matrix-matched calibration curves was prepared in the range of 0.01 – 100 ng/ml. The linearity range (r ≥ 0.995) of the curves were analyte dependent. Lower limits of quantification can be achieved for some compounds.EAD is an alternative fragmentation mode that produces unique fragmentation ions, which can be used as diagnostic ions for compound differentiation. The creation of our own library was important because in the developed method MS/MS spectrum is collected for the optimized collision energy (CE), while the spectra deposited in commercial databases are generated at a constant CE value and contain only a few of the tested substances. Moreover, there are no commercial databases for EAD fragmentation data.The created LC-MS/MS method is characterized not only by high sensitivity, but by using a HR-MS/MS, it enables determination of additional factors confirming the presence of the compound including mass defect for precursor and quantifier ion, isotope distribution and finally library matching.In addition, the presence of Zeno ion trap gives better quality and sensitivity of the fragmentation spectrum. The developed LC-HR-MS/MS method is characterized by a much better specificity compared to the analyzes performed on any LR device.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_HR_MS_MS_EAD_and_CID_fragmentation_leads_to_selective_sensitive_and_reliable_quantitation_of_anabolic_steroids_in_urine_Table_4_bc828cc2bb.jpg" alt="Bioanalytic: Table 4. Example of multi-level confirmation and scoring by the anabolic AAS method in urine using ZENO TOF 7600 LC-MS/MS system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_HR_MS_MS_EAD_and_CID_fragmentation_leads_to_selective_sensitive_and_reliable_quantitation_of_anabolic_steroids_in_urine_Table_4_bc828cc2bb.jpg 245w," sizes="100vw" width="2702" height="804"><h4>Novel aspect</h4>Sensitive and selective LC-HR-MS/MS method using CID and EAD fragmentation modes for anabolic steroids quantitation in human urine with confidence.

HR-MS/MS EAD and CID fragmentation leads to selective, sensitive and reliable quantitation of anabolic steroids in urine

<blockquote>Maciej Stopa1, 2; Rafał Szewczyk1, 2; Adrianna Radulska3, Anna Lenartowicz2; Julia Mironenka2; Adrian Soboń1, 2; Katarzyna Krupczyńska-Stopa1, 2; Tomasz Borkowski3; Ewelina Marciniak3, Leszek Kalinowski31<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315">Bioanalytic</a> Sp. z o.o., Gdansk, Poland; 2LabExperts Sp. z o.o., Gdansk, Poland; 3 Medical University of Gdansk, Gdansk, Poland</blockquote><h4>Abstract</h4>Cardiac arrhythmia affects approximately 12.6% of people over the age of 65. Ventricular arrhythmias are considered as responsible for 75% to 80% of sudden cardiac deaths. Therapy of the disease include ablation, use of cardioverter or defibrillator and pharmacological treatment where the key for successful therapy lays in maintaining the necessary concentration of drugs. The goal can be achieved by therapeutic drug monitoring (TDM). Developed assay named “CardioCarePack” is based on the use of capillary blood collected and dried at home by a patient with volumetric absorptive microsampling (VAMS), quantitative analysis of selected drugs and their metabolites and a telemedical system that integrates all data between a doctor, patient and laboratory supporting the therapy process. CardioCarePack is ready for commercialization.<h4>Methods</h4>Blood collected with a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?distributorItems=315&amp;search=MITRA">MITRA® microsampling device</a> (20 µl) is prepared with full validated protocol that include sonication with 0.1% formic acid in water followed by acetonitrile extraction, evaporation under nitrogen stream and resuspension in LC mobile phase. Sample is subjected to LC-MS/MS analysis in positive ionization MRM mode with QTRAP 5500+ mass spectrometer (SCIEX) or similar one. Analysis covers simultaneous quantitation of 15 drugs: Sotalol, Metoprolol, Bisoprolol, Propafenone, Carvedilol, Nebivolol, Amiodarone, Digoxin, Perindopril, Ramipril, Eplerenone, Spironolactone, Zofenopril and 2 metabolites: 5-hydroxypropafenone and Desethylamiodarone. Results are placed in a server-based telemedical system where depending on accession rights history, doses, therapeutic index flagging, cardiograms, sample tracking and many other data are available for patient, doctor and laboratory staff, respectively.<h4>Preliminary Data</h4>The project involved more than 300 patients who had been monitored for 2 years during regular pharmacological therapy. Every half a year during a visit in medical facility venous blood for serum testing and capillary blood on the MITRA® sorbent were collected. Between the visits patients were collecting samples by VAMS themselves at home. All samples were analyzed by fully validated procedure where among the others linearity (r ≥ 0.995), accuracy (80 – 120%), reproducibility (%CV ≤ 15%) for both MITRA® and serum samples were calculated. The LC-MS/MS method developed for 17 compounds covers therapeutic range of the tested drugs: 0.25 – 25 ng/ml (Digoxin and Nebivolol) 2.5 – 250 ng/ml (Metoprolol, Bisoprolol, Propafenone, Carvedilol, Perindopril, Ramipril, Spironolactone, Zofenopril and 25 – 2500 ng/ml (Sotalol, Desethylamiodarone, Eplerenone, Amiodarone, 5-hydroxypropafenone). Results generated during LC-MS/MS analysis were analyzed with SCIEX OS 1.7 software.On the basis of samples collected during 4 visits in medical facility a correlation between drugs concentration in venous blood (serum) and capillary blood (MITRA®) (S/M) was calculated. For some compounds S/M ratio was close to 1 (ex. Sotalol – 0.95, %CV – 5.12%, Nebivolol – 1.03, %CV – 3.17%) or moderately to more than 2-fold different (ex. Digoxin – 0.69, %CV – 1.46%, Ramipril – 1.18, %CV – 11.1, Perindopril – 1.86, %CV – 4.73%, Amiodarone – 2.17, %CV – 10.78). The correlation factors are statistically significant (p &lt; 0.05) and can be used for accurate concentration estimation in venous blood.Developed telemedicine system integrate CardioCarePack service – a patient, doctor and LC-MS/MS laboratory. The system helps in Doctor’s supervision over the patient’s condition on the basis of collected within the system data ex.: autonomous verification of the therapeutic index with flagging, visualization of results, tracking samples on the patient-doctor line, schedule of visits, results history and many more.<h4>Novel aspect</h4>A modern telemedicine system for TDM of cardiological drugs based on LC-MS/MS analysis of samples collected at home with VAMS.

CardioCarePack – personalized medicine system to improve live quality of patients suffering from cardiac arrhythmias

<blockquote>Anna Lenartowicz (1); Julia Mironenka (1); Adrian Soboń (1,2); Rafał Szewczyk (1,2); Katarzyna Krupczyńska-Stopa (1,2); Maciej Stopa (1,2); Andrzej Kwaśnica (3) 1 – LabExperts sp. z o. o., Gdańsk, Poland; 2 – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/315">Bioanalytic</a> sp. z o. o., Gdańsk, Poland; 3 – Lab4Tox sp. z o. o., Wrocław, Poland</blockquote><h4>Introduction</h4>Compound presence confirmation is one of the key aspects of targeted and quantitative analysis in forensics applications. High resolution tandem mass spectrometry (HR-MS/MS) may become a major tool for this kind of analysis because of its mass accuracy resulting in unparallel specificity, all combined with broad linearity and scanning speed achieved in the newest hardware on the market. In this work we show a modern HR-MS/MS method for a sensitive detection of 27 analytes with high confidence in venous blood and blood collected on a volumetric absorptive microsampling (VAMS) probe. Analytes include: Tramadol, Morphine, Fentanyl, Methadone, Codeine, Alprazolam, Diazepam, Flunitrazepam, Lorazepam, Oxazepam, Zolpiclone, Clonazepam, Nordiazepam, Zolpidem, Hydroxyzine, Amphetamine, Methamphetamine, MDA, MDMA, MDEA, THC, Cocaine, 6-acetylmorphine, 7-aminoflunitrazepam, 7-aminoclonazepam, Benzoylecgonine, THC-COOH.<h4>Materials and Methods</h4>Briefly, sample preparation included: spiking of internal standards, protein precipitation and extraction using acetonitrile:methanol (1:1) with 0.1% formic acid, evaporation under nitrogen stream and reconstitution in LC mobile phase. Compounds were analyzed using reversed-phase chromatography on ExionAC LC coupled with ZenoTOF 7600 (SCIEX) operating in positive electrospray ionization scheduled MRMhr mode acquiring full MS/MS spectra for each compound. Developed processing method use multi-level confirmation and combined scoring based on HR data where result is affected by 5 criteria that have different decision weights: MS/MS library confirmation (50%), precursor ion mass defect (20%), quantifier ion mass defect (20%), precursor ion isotope pattern (5%) and retention time (5%). Each criterium contributes in the combined score calculation and has its own range of 3-level acceptance in ppm, % or seconds, respectively. The cut-off point of combined score is estimated at 70% after series of analysis and allows flawless reporting of compounds from DRUID list. Data were processed in SCIEX OS software.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytics_Figure_1_Procedure_of_blood_samples_preparation_ed1d01bbb3.jpg" alt="Bioanalytics: Figure 1. Procedure of blood samples preparation." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytics_Figure_1_Procedure_of_blood_samples_preparation_ed1d01bbb3.jpg 167w," sizes="100vw" width="1256" height="1177"><h4>Results</h4>The method was validated separately for blood and blood collected on VAMS and fulfilled the following criteria for each analyte: linearity (R ≥ 0.995), working range (LLOQ – 50 ng/ml), reproducibility (%CV ≤ 15%), accuracy (80 – 120%) and recovery (80 – 120%). For majority of analytes included in the method LLOQ was in the range 0.01- 0.05 ng/ml (ex. Diazepam, Flunitrazepam, Kodeine, Morphine, MDA) for blood and 0.05-0.1 ng/ml for VAMS sampler. For analytes such as Tramadol, Fentanyl, Methadone and Cocaine the LLOQ ranged between 0.001- 0.005 ng/ml for blood and 0.01-0.05 for VAMS. The method was successfully applied to routine analysis of blood samples.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytics_Table_1_Parameters_applied_in_LC_MS_MS_method_a71f5fed46.jpg" alt="Bioanalytics: Table 1. Parameters applied in LC-MS_MS method" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytics_Table_1_Parameters_applied_in_LC_MS_MS_method_a71f5fed46.jpg 245w," sizes="100vw" width="1162" height="323"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytics_Figure_2_Chromatogram_presenting_LC_MS_MS_analysis_of_27_psychoactiv_compounds_from_DRUID_list_b369671a31.jpg" alt="Bioanalytics: Figure 2. Chromatogram presenting LC-MS_MS analysis of 27 psychoactiv compounds from DRUID list." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytics_Figure_2_Chromatogram_presenting_LC_MS_MS_analysis_of_27_psychoactiv_compounds_from_DRUID_list_b369671a31.jpg 245w," sizes="100vw" width="1884" height="753"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytics_Figure_3_Criteria_applied_in_developed_processing_method_using_a_multi_level_confirmation_and_combined_scoring_based_on_HR_data_e6f93aa598.jpg" alt="Bioanalytics: Figure 3. Criteria applied in developed processing method using a multi-level confirmation and combined scoring based on HR data." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytics_Figure_3_Criteria_applied_in_developed_processing_method_using_a_multi_level_confirmation_and_combined_scoring_based_on_HR_data_e6f93aa598.jpg 245w," sizes="100vw" width="1893" height="1060"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytics_Table_2_Results_of_quantitative_methods_validation_74781e6dcf.jpg" alt="Bioanalytics: Table 2. Results of quantitative methods validation." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytics_Table_2_Results_of_quantitative_methods_validation_74781e6dcf.jpg 162w," sizes="100vw" width="1162" height="1120"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytics_Figure_4_Example_of_false_positive_result_exclusion_for_Metamphetamine_in_unspiked_venous_blood_sample_due_to_multi_level_confirmation_method_cd0fc2540a.jpg" alt="Bioanalytics: Figure 4. Example of false positive result exclusion for Metamphetamine in unspiked venous blood sample due to multi-level confirmation method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytics_Figure_4_Example_of_false_positive_result_exclusion_for_Metamphetamine_in_unspiked_venous_blood_sample_due_to_multi_level_confirmation_method_cd0fc2540a.jpg 147w," sizes="100vw" width="1173" height="1246"><h4>Discussions and Conclusions</h4>A quantitative LC-MS/MS method was validated according to the international recommendations. The greatest advantage of presented method is its high specificity and extremely low Limits of Quantitation. Most of described in literature LC-MS/MS methods used for psychoactive compounds quantification are based on MRM scanning mode with triple quadrupole instruments. Such approach does not allow to obtain sufficiently high specificity because of difficult data interpretation at low concentrations of analytes in real-life samples where MRM ratio and matrix interferences leads to data misinterpretation. Developed method allows sensitive, specific and false positive resistant HR-MS/MS quantitation of 27 psychoactive compounds in blood and blood collected by VAMS.

How sure you can be of the results of quantification of psychoactive compounds in blood?

High Performance Liquid Chromatography (HPLC) is widely recognized as a premier chemical analytical technique in many industries vital for human existence. Its ability to separate and detect numerous classes of organic and inorganic compounds, as well as determine their concentration in various matrices make HPLC by far the most popular analytical tool in the world.However, its high costs, operational complexity, and the need for highly qualified specialists present significant challenges.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1242"> Alltesta™ Automated Analyzer</a>, simplifies the HPLC analysis process, making it more accessible without compromising quality or accuracy.<h4>Challenge</h4>The laboratory in question faced the challenges of:<ul><li>High costs of HPLC instrumentation and separation columns</li><li>Significant space requirements – traditional HPLC systems take up a large amount of laboratory space, limiting flexibility and accessibility.</li><li>The need for a specialized laboratory environment and employ highly educated and well-trained scientific personnel</li><li>Extensive analytical method development, even for simple analyses.&nbsp;</li></ul>These factors limit the accessibility of HPLC and restrict its use outside specialized laboratories.<h4>Solution: Alltesta™ Automated Analyzer</h4>The laboratory decided to explore SIELC’s offerings and quickly found an Alltesta™ Automated Analyzer that met their requirements. Designed to simplify HPLC workflows and address key user challenges, the system offers:<ul><li>Compact size – Alltesta™ Automated Analyzer features a space-saving design, making it ideal for small laboratories and research environments with limited workspace.</li><li>Ease of use – operates from a standard power outlet and connects to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1250">HPLC.cloud</a> via Wi-Fi, Ethernet, or a mobile network.</li><li>Automated method setup – The company SIELC removes the major burden of analytical method development from an inexperienced system operator, allowing them to focus on obtaining analytical results in minimal time. This is accomplished by SIELC’s experienced staff, who identify the optimal columns, solvents (mobile phases), and gradient parameters for a particular analysis—free of charge for clients. The company also maintains a vast library of analytical methods for numerous compounds and produces a wide variety of HPLC columns to support their implementation.</li><li>Cloud-based control and data processing – server-hosted software enables remote operation and provides analytical results in an easy-to-read format.</li><li>Advanced HPLC technologies – the system features a pulse-free syringe pump (up to 5000 psi) (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247">Alltesta™ Mini Syringe Pump</a>), an autosampler (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244">Alltesta™ Mini-Autosampler</a>), and a multi-wavelength UV detector (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248">Alltesta™ Mini UV-Vis Detector</a>), ensuring reliable analytical performance.</li></ul><figure class="image"><img style="aspect-ratio:1567/972;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg" alt="Bioanalytic: SIELC Alltesta™ Gradient Automated Analyzer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg 245w," sizes="100vw" width="1567" height="972"></figure><h4>Results &amp; Impact&nbsp;</h4>The implementation of Alltesta Automated Analyzer has significantly streamlined the HPLC analysis process. Laboratory report:<ul><li>Reduced costs by minimizing expenses on consumables and cutting method development time.</li><li>Simplified workflow – even individuals without specialized training can perform analyses and obtain accurate results.</li><li>System reliability – extensive testing has shown no hardware or software failures.</li><li>Enhanced data security – HPLC.cloud ensures safe data storage, reliable processing, and secure remote access.&nbsp;</li></ul>Dr. Michael Z. Kagan, PhD, Senior Chromatographer with 28 years of experience at Pfizer, AbbVie, and Coca-Cola, highlights:&nbsp;"During six months of testing, I did not encounter a single hardware or software issue. The system is easy to use, offers flexible column and method configurations, and benefits from expert chromatographic support, making Alltesta a reliable and user-friendly solution."<h4>Conclusion</h4>Alltesta™ Automated Analyzer removes the main barriers associated with HPLC analysis, making it accessible, simple, and efficient. With automation and cloud-based technologies, users can focus on obtaining results rather than dealing with complex instrument settings.

Optimizing HPLC Analysis with Alltesta™ Automated Analyzer

SIELC has developed the first and only HPLC software that is accessible via the cloud - <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1250">HPLC.cloud</a>.<h4>&nbsp;Special Features:</h4><ul><li>Cloud Integration: Real-time data streaming and storage on HPLC.cloud ensure your experiment data is always backed up and accessible.</li><li>Remote Access: Control and monitor your system from anywhere — lab, office, or on the go — using a PC, tablet, or phone.</li><li>Data Security: All your results are stored in a centralized, secure location — so no data is ever lost.</li><li>Sync Capabilities: Chromatograms are synced with HPLC.cloud every second, providing real-time experiment updates.</li></ul><h4>Key Functionality:</h4><ul><li>Real-time Chromatogram Streaming: Watch live data as it’s being collected.</li><li>Sequence Building and Recording: Build, record, and store experiment sequences.</li><li>Method Storage: Save and reuse methods for consistency and efficiency.</li><li>Data Accessibility: Store all your sample and result data securely in the cloud, accessible anytime.</li><li>Remote Control: Start, stop, or modify analyses from any internet-connected device.</li><li>Comprehensive Data Analysis: Calibrate, analyze, and quantify directly within the cloud interface.</li><li>Mobile and PC Access: Use on PC, tablet, or smartphone for maximum flexibility.</li><li>Online Data Search: Easily search and access your data from anywhere.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg" alt="SIELC: SIELC Software – HPLC.cloud" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Software_HPLC_cloud_1_441d304d5d.jpg 225w," sizes="100vw" width="801" height="554">No software installation. No USBs. No data loss.

How to Use HPLC Cloud - A Complete Guide

<h3>Summary</h3>High Performance Liquid Chromatography (HPLC) is an essential analytical tool in laboratories across various industries, enabling precise separation and detection of complex compounds. While traditional diode-array HPLC systems are valuable for method development, they can be unnecessary when routine analyses are performed. Our laboratory sought a streamlined solution for standardized separations using a pre-optimized protocol—one that was intuitive, space-efficient, and required minimal hands-on expertise.&nbsp;We adopted the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1242">SIELC Alltesta™ Automated Analyzer</a> compact HPLC system with LED-based detection, which provided an ideal balance of performance, ease of use, and automation. This system significantly improved workflow efficiency, reduced operational demands, and empowered even non-specialist staff to reliably perform high-quality analyses.<h3>Challenge</h3>Our laboratory faced several key constraints that limited the scalability and efficiency of HPLC analyses:<ul><li>Staffing limitations – Traditional HPLC setups often require experienced analytical chemists, creating bottlenecks in routine workflows.</li><li>Space constraints – Larger HPLC systems demand significant bench space, reducing flexibility in smaller lab environments.</li><li>Multitasking difficulties – Monitoring experiments manually detracted from other research activities, limiting productivity.</li></ul>To overcome these challenges, we sought an HPLC system that could provide accurate, repeatable results without requiring specialized personnel or extensive lab resources.<h3>Solution: SIELC’s compact LED-Based Alltesta™ Automated Analyzer</h3>After evaluating available options, our laboratory implemented a compact HPLC system featuring LED detection. This choice allowed us to optimize efficiency while maintaining analytical integrity. The key advantages of this system included:<ul><li>Compact footprint – The small-scale design seamlessly integrated into our lab setup, freeing up valuable workspace.</li><li>User-friendly interface – Intuitive software enabled streamlined control and monitoring, even when multitasking on other unrelated tasks.</li><li>Automated sampling – The high-performance autosampler facilitated unattended operation, reducing manual intervention and improving throughput.</li><li>Simplified detection – LED-based detection was ideal for our needs, providing highly stable and reproducible results for pre-optimized protocols.</li><li>Versatile usability – Interns and non-specialist personnel could operate the system with minimal supervision, lowering staffing requirements.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg" alt="Bioanalytic: Enhancing Routine HPLC Analysis with a Compact LED-Based System: SIELC Alltesta™ Gradient Automated Analyzer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_cover_image_b8131d2b08.jpg 245w," sizes="100vw" width="1567" height="972"><h3>Results &amp; Impact</h3>The adoption of the compact LED-based HPLC system led to substantial improvements in laboratory efficiency and accessibility. Key benefits included:<ul><li>Reduced staffing demands – The system’s simplicity enabled non-specialist operators to perform analyses, minimizing reliance on dedicated analytical chemists.</li><li>Increased productivity – Automated sampling and remote monitoring capabilities allowed researchers to focus on other projects while experiments ran in the background.</li><li>Optimized lab space – The system’s compact design enabled seamless integration into our lab, maximizing workspace utility.</li><li>Reliable performance – Routine analyses were executed with high precision and reproducibility, ensuring consistent experimental outcomes.</li></ul><blockquote>Dr. Nikolai Braun, PhD, Research Scientist at Luna Labs, highlights:"Compact, intuitive, and reliable: the Alltesta™ Automated Analyzer has everything you need for routine measurements, revolutionizing what you expect from an HPLC system."</blockquote><h3>Conclusion</h3>By integrating a compact LED-based Alltesta™ Automated Analyzer into our workflow, we successfully enhanced efficiency, reduced operational complexity, and expanded analytical capabilities beyond specialized personnel. This case study highlights the transformative impact of accessible, intuitive chromatography solutions—empowering laboratories to maintain high-quality analyses while minimizing overhead costs and staffing requirements.

Enhancing Routine HPLC Analysis with a Compact LED-Based System

<h4>A New Standard in HPLC Column Connection by SIELC</h4>SIELC Technologies, a pioneer in chromatography innovation, introduces a single-ended HPLC column — a revolutionary new design that redefines how columns are connected and used in high-performance liquid chromatography. For decades, laboratories have relied on the same standard column format — and have dealt with the same recurring problems: leaky fittings, clogged junctions, dead volume, and the constant hassle of tubing and connection selection.SIELC has developed a single-ended HPLC column design.This new system redefines how columns are installed, used, and replaced — streamlining your workflow and eliminating common points of failure.<h5>Key Benefits of the New Design:</h5><ul><li>No fittings or tubing required — eliminates leaks, complex installation, and the need to match tubing length or ID.</li><li>Zero dead volume thanks to a direct connection via a dedicated adapter.</li><li>Leak containment — in case of pressure failure, solvent remains inside the adapter with no external leakage.</li><li>Tool-free column replacement — disconnect the column without touching the fittings.</li><li>Aluminum body — ensures efficient heat transfer and provides a smooth, label-friendly surface even on the shortest columns.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_HPLC_column_3af7eb512a.jpg" alt="SIELC: HPLC column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_HPLC_column_3af7eb512a.jpg 171w," sizes="100vw" width="1600" height="1459"><h4>HPLC Column Adapter</h4>The SIELC adapter is a key component of the new single-ended column system, designed to eliminate leaks, dead volume, and connection complexity in HPLC setups.This adapter is an engineering solution that ensures perfect sealing, precise flow alignment, and reliable performance under high pressure — all without traditional fittings, ferrules, or tools.<ul><li>Dimensions: 21 × 21 × 26 mm (0.83 × 0.83 × 1.02 in)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_HPLC_Column_Adapter_8495244726.jpg" alt="SIELC: HPLC Column Adapter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_HPLC_Column_Adapter_8495244726.jpg 165w," sizes="100vw" width="918" height="868"><blockquote>Special offer: First-time buyers receive a Free Single-Column Adapter with their purchase. Experience the new connection standard — no fittings, no leaks, no extra cost.</blockquote><h4>How the Single-Ended HPLC Column Works?</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_How_the_Single_Ended_HPLC_Column_Works_24ebf8b072.jpg" alt="SIELC: How the Single-Ended HPLC Column Works?" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_How_the_Single_Ended_HPLC_Column_Works_24ebf8b072.jpg 245w," sizes="100vw" width="1600" height="474">As the mobile phase enters through the MP Inlet, it flows into the adapter’s internal capillary aligned with the column axis. When the mobile phase passes through the column packing bed, it exits through the bypass channel connected with the MP Outlet.This design eliminates dead volume, prevents mobile-phase leakage, and provides a simple way to remove and replace the column when needed.<h3>Dual-Ended vs. Single-Ended HPLC Columns</h3><h4>Dual-Ended HPLC Columns</h4><ul><li>Column connections: Dual-ended HPLC columns have two separate ends. Each connection requires fittings, ferrules, and tubing to connect the column to the LC system.</li><li>Installation: Installation requires manual alignment of fittings and precise adjustment of tubing length to minimize dead volume. This process can be time-consuming and is prone to user error.</li><li>Dead volume: Dead volume is typically present at both the inlet and outlet connections. These junctions can cause peak broadening and reduced chromatographic efficiency.</li><li>Leakage risk: Leakage risk is relatively high because solvent connections rely on ferrules and fittings that may loosen or wear over time.</li><li>System integration: Dual-ended columns require separate tubing and careful fitting when installed in column heaters or coolers.</li><li>Thermal performance: Heat transfer may be less uniform due to multiple connections and tubing interfaces.</li><li>Guard column option: Using a guard column requires additional hardware and extra connections, which can introduce additional dead volume into the system.</li><li>Available phases: Dual-ended columns are typically available in standard stationary phases such as C18, HILIC, ion exchange, and others.</li></ul><h4>Single-Ended HPLC Columns</h4><ul><li>Column connections: Single-ended columns use a single coaxial connection with an integrated adapter. No fittings, ferrules, or tubing are required.</li><li>Installation: The adapter is installed only once. After that, the column can be replaced in seconds without tools, making maintenance faster and simpler.</li><li>Dead volume: Near-zero dead volume is achieved because the internal capillaries are precisely aligned within the adapter.</li><li>Leakage risk: The mobile phase is fully contained inside the adapter, creating a leak-free design even under high pressure conditions.</li><li>System integration: A compact stainless-steel adapter allows easy installation in column heaters or coolers.</li><li>Thermal performance: The aluminum outer body provides efficient and uniform heat transfer across the column.</li><li>Guard column option: An integrated zero-dead-volume guard column option is available, allowing quick cartridge replacement without tools.</li><li>Available phases: All SIELC phases as well as traditional chromatographic phases (C18, HILIC, ion exchange, mixed-mode, BIST™, etc.) are available in this format.</li></ul><h4>Adapter for Guard Column</h4>If your workflow requires a guard column or a filter to be installed inline with the main column, then a specialized adapter with a guard option should be used.The SIELC HPLC Single-Column Guard protects the analytical column by capturing particulates and strongly retained contaminants before they reach the stationary phase. Its unique direct-insert design provides a zero-dead-volume connection and allows quick, tool-free replacement of a used guard with a new one. Guards are available for all SIELC stationary phase types — Primesep™, Newcrom™, Chromni™, PEI™, BIST™, and for generic phases such as C18, HILIC and IE.<h5>Guard Cartridges (2 per pack)</h5><ul><li>Guard Adapter — Dimensions: 21 × 21 × 42 mm (0.83 × 0.83 × 1.65 in)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_guard_cartridges_4077d1edf2.jpg" alt="SIELC: Guard cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_guard_cartridges_4077d1edf2.jpg 245w," sizes="100vw" width="768" height="262"><h4>Why This Matters</h4>This new column system removes the guesswork and fragility from high-pressure liquid chromatography. By eliminating traditional high-pressure fittings and minimizing dead volume, you gain more consistent results, lower the risk of system failure, and reduce downtime.Whether you’re running routine analyses or complex separations, this design enhances reliability and usability — so you can focus on your science, not your setup.<blockquote><h5>All SIELC and Traditional Columns and Guards Available in a New Format</h5>C18, HILIC, Ion Exchange, Mixed-Mode, Ion Exclusion, Protein, BIST, and more.</blockquote><blockquote>📄 Want to learn more? <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Single_ended_column_new_2_1_b80be5f107.pdf">Download our brochure</a> to learn about a single-ended HPLC column design.</blockquote>

Single-Ended HPLC Column by SIELC

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Bioanalytic started its operations in 1998. The company was born from a passion for science and the need to provide innovative solutions in the laboratory equipment market, from a small business to a dynamically developing company with an established position.

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