In-Source Fragmentation Searches in NIST26 Chromatogram Window
Learn how to recognize and search in-source fragmentation spectra in NIST26 using chromatogram results, Score (Unfiltered), and MS/MS Library Search for compound identification.
Processing MS/MS Data in NIST26 Chromatogram Window
A practical guide to processing LC-MS/MS data in the NIST26 Chromatogram Window, from parameter setup and library searching to identification review and result export.
NIST26 Advanced Course LC-MS/MS Identifications of Unknowns
Discover the new NIST26 LC-MS/MS workflow with integrated chromatogram analysis, expanded MS/MS libraries, hybrid searching, and advanced tools for confident unknown compound identification.
Lib Search: Cleaning Up Workspace and Temporary HLM Files in NIST26clean up
Learn how NIST MS Search stores library search history, what HLM files are used for, and how to automatically remove temporary files to keep your workspace clean and organized.
Raw Data Files for EI Low Resolution in the Identifications Folder
Discover what the NIST Identification Folder contains during low-resolution EI GC-MS processing. Learn how TIC, ELU, FIN, TSV, RUN, and LOG files support deconvolution and library searching.
MS Interpreter for EI Accurate Mass Data, Correlating Structure to m/z
Learn how to use MS Interpreter in NIST 26 to interpret accurate-mass EI GC-MS spectra, verify library hits, compare candidate structures, and improve compound identification.
RI Calibration in NIST26 Chromatogram and Applying to Calculating RI in Samples
Learn how to create retention index calibrations in NIST 26, calculate RI values automatically, and improve GC-MS compound identification by combining spectral library searching with retention indices.
Lib Search: Similarity EI Hybrid Search for Spectra Sent from Chromatogram Window
Learn how NIST 26 Hybrid Search expands EI GC-MS library searching by identifying structurally related compounds using mass-shifted fragment matching, DeltaMass analysis, and MS Interpreter validation.
Normal EI Search for Spectra Sent from Chromatogram Window
Learn how to use NIST 26 Lib Search with spectra sent from the Chromatogram Window, compare Full Spectrum and Reverse searches, and improve EI GC-MS identifications.
Processing of Accurate Mass GC-MS with NIST26 Chromatogram
Learn how to process accurate-mass GC-MS data in NIST 26 using integrated deconvolution, library searching, MS Interpreter validation, filtering tools, and Excel reporting workflows.
