Comprehensive analysis of low abundant mannose glycopeptides in peptide mapping of adalimumab

Optimized digestion procedure and characterization for monoclonal antibodies and proteins by CESI-MS Featuring CESI 8000 Plus and TripleTOF® 6600+ LC-MS/MS System Zuzana Demianova1, Anna Lou1 and Sahana Mollah2 1 SCIEX, Brea, CA; 2 SCIEX, Redwood City, CA Peptide mapping by…

How to Determine Glycan Profiles of Biopharmaceuticals from Peptide Mapping Data A new and sensitive approach combining BioPharma Compass, PASEF and VIP-HESI Abstract Glycosylation is a common critical quality attribute (CQA) of therapeutic proteins and needs to be characterized during…

APPLICATION NOTE 73672 Accelerating monoclonal antibody peptide mapping with high acquisition speed Orbitrap-based MS Authors: Phil Widdowson, Amy Claydon, Tom Buchanan Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Peptide mapping, monoclonal antibody, mAb, post-translational modification, PTM, high throughput analysis, golimumab,…

Application Note Biotherapeutics and Biosimilars Glycopeptide Characterization for Various Monoclonal Antibodies Using the Agilent 6545XT AdvanceBio LC/Q-TOF Author David L. Wong Agilent Technologies, Inc. Introduction Monoclonal antibodies (mAbs) and their derivatives represent a very complex but important class of biopharmaceutical…