Rapid monitoring of mAb aggregates during the purification process development of therapeutic mAbs using a modified Orbitrap hybrid MS

Aggregates analysis of a bispecific antibody using Orbitrap Excedion Pro BioPharma Hybrid mass spectrometer with extended mass range Xiaoxi Zhang1, Reiko Kiyonami2, Weijing Liu3, Xuepu Li1, Min Du2. 1 Thermo Fisher Scientific, Shanghai, China. 2 Thermo Fisher Scientific, Massachusetts, US…

Applying UHPLC HRAM MS-MS/MS method to assess host cell protein clearance during the purification process development of therapeutic mAbs Reiko Kiyonami1, Ying Chen2, AI De Leon2, Min Du1, 1Thermo Fisher Scientific, Lexington, MA; 2Thermo Fisher Scientific, Bedford, MA Introduction Mass…

NATIVE MASS SPECTROMETRY DISTINGUISHES HOMO VS HETERO NON-COVALENT DIMERIC AGGREGATES OF MONOCLONAL ANTIBODIES Catalin Doneanu1, Hui Wei2, Richard Ludwig3, Laurel Hoffman2, Li Tao2, Ying Qing Yu1 and Weibin Chen1 1 Waters Corporation, Milford, MA; 2Bristol-Myers Squibb, Pennington, NJ; 3Bristol-Myers Squibb,…

Agilent Biocolumns Aggregate/Fragment Analysis Application Compendium Contents Background 2 Getting Started 3 How to Guide - Size Exclusion Chromatography for Biomolecule Analysis - 5991-3651EN 4 Featured Application Notes Elevate Your mAb Aggregate Analysis - 5994-2709EN 1 22 22 High-Resolution, High-Throughput…