Mass spectrometry-based structural analysis of oligonucleotide DNA duplexes

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…

Application Note Biopharma Complementary LC/MS and UV‑Vis Spectrophotometry for siRNA Quality Control and Thermal Stability Assessment Authors Abstract Yulan Bian and Ravindra Gudihal Agilent Technologies, Inc. Small interfering RNAs (siRNAs) are increasingly important therapeutic agents, which require robust analytical strategies…

Application Note Investigating the Strength of the Sense and Antisense Oligonucleotide Interaction to Form siRNA Duplexes Using the BioAccord™ LC-MS System Catalin Doneanu, Ying Qing Yu Waters Corporation, United States Published on August 03, 2026 Abstract A hydrophilic interaction liquid…

Liquid Chromatograph Mass Spectrometer LCMS-9050 Application News Reversed-Phase Ion-Pair LC/MS Analysis of siRNA under Denaturing and Non-Denaturing Conditions Junna Nakazono User Benefits  The LCMS-9050 quadrupole time-of-flight mass spectrometer can be used to characterize siRNA.  The elution of siRNA…