Analysis of the global histone modification landscape in mouse tissue using nano C18-monolithic column and timsTOF HT

Top‐Down Mapping of Protein Modifications by Multimodal MSⁿ on the New timsOmni Platform Francis Berthias1, Nurgül Bilgin2, Athanasios Smyrnakis3, Mariangela Kosmopoulou3, Christian Albers4, Jasmin Mecinović2, Dimitris Papanastasiou3, and Ole N. Jensen1 1 Department of Biochemistry and Molecular Biology; 2 Department…

Sample Preparation Core histones were acid-extracted from cultured HEK293T cells, column-purified by ion exchange, and desalted by perchloric acid precipitation. The purified histones were prepared for MS using a hybrid chemically derivatization strategy, whereby an initial conversion of free lysines…

ASMS 2025 timsplot: A Python Shiny App for Visualizing timsTOF Proteomics Results Zachary J. Kirsch1, Manubhai Kadayil Prabhakaran1, Diego Assis1, Ruben Shrestha2, and Matthew Willetts1 1Bruker Scientific, Billerica, MA, 2Bruker Scientific, San Jose, CA Introduction The timsTOF platform has garnered…

High sensitivity lysine acetylation profiling with Trapped Ion Mobility Spectrometry and PASEF ASMS 2020, WP 507 Xianming Liu1, Jun Zhu2, Chen Bu2, Lianqi Zhou2, Ning Chen3, Xiaoxian Du1, Shourjo Ghose4, Stephanie KasparSchoenefeld5, Heiner Koch5. 1Bruker Daltonics, Shanghai, China 2PTM Biolabs…