Titer and charge-based heterogeneity multiattributemonitoring of mAbsin cell culture harvest using 2D ProACEX MS

Poster Reprint ASMS 2023 Poster number MP 625 A native multi-dimensional monitoring workflow for at-line characterization of mAb titer, size, charge, and glycoform heterogeneities in cell culture supernatant Sanghati Bhattacharya1, Shashank Jain2 , Vadiraja Bhat3, Srishti Joshi1, Anurag S. Rathore1…

Poster Reprint ASMS 2025 Poster number TP 121 An Online Two-Dimensional Approach to Characterizing the Charge-Based Heterogeneity of Recombinant Monoclonal Antibodies Using a 2D-CEX– AEX–MS Workflow Anurag Singh Rathore1; Kunal Krrishna1; Vadiraja Bhat2; Sunil Kumar1; Vineela Peruri1; John Sausen3 1Department…

Application Note Biopharma/Pharma Determination of Multiple Attributes of Monoclonal Antibodies Simultaneous and parallel multi-attribute analysis using 3D-LC/MS with 2D multimethod option mAb SEC Size variants Protein A CEX LC/MS mAb Titer Charge variants Mol wt (MW) AA sequence PTM Cell…

Application note | 003310 Biopharma Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS Authors Application benefits Maria Grübner , Kai Scheffler , 1 1 • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of…