A native multi-dimensional monitoring workflow for at-line characterization of mAb titer, size, charge, and glycoform heterogeneities in cell culture supernatant

Biopharma Compendium Accelerate Your CQA Efficiency and Approach it with Multi-Attribute Methodology Breakthroughs to Increase the Efficiency of Critical Quality Attribute (CQA) Testing and Allow for a Multi- Attribute Method (MAM) Production of therapeutic monoclonal antibodies (mAbs) relies on living…

Application note | 003310 Biopharma Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS Authors Application benefits Maria Grübner , Kai Scheffler , 1 1 • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of…

Application Note Biopharma/Pharma Determination of Multiple Attributes of Monoclonal Antibodies Simultaneous and parallel multi-attribute analysis using 3D-LC/MS with 2D multimethod option mAb SEC Size variants Protein A CEX LC/MS mAb Titer Charge variants Mol wt (MW) AA sequence PTM Cell…

Application Note Pharma & Biopharma Multi-Attribute Analysis of Monoclonal Antibodies Using the Agilent InfinityLab 2D-LC Solution and Q-TOF MS Authors Gerd Vanhoenacker, Isabel Vandenheede, Pat Sandra, and Koen Sandra Research Institute for Chromatography (RIC) Kortrijk, Belgium Sonja Krieger, Sonja Schneider,…