An Online Two-Dimensional Approach to Characterizing the Charge-Based Heterogeneity of Recombinant Monoclonal Antibodies Using a 2D-CEX–AEX–MS Workflow

Poster Reprint ASMS 2025 Poster number TP 122 Titer and charge-based heterogeneity multiattribute monitoring of mAbs in cell culture harvest using 2D ProA CEX MS Anurag Singh Rathore1; KUNAL KRISHNA1; Vadiraja Bhat2; Sunil Kumar1; Deepika Sarin1; Shashank Jain3 1Department of…

Application Note Biopharma/Pharma Determination of Multiple Attributes of Monoclonal Antibodies Simultaneous and parallel multi-attribute analysis using 3D-LC/MS with 2D multimethod option mAb SEC Size variants Protein A CEX LC/MS mAb Titer Charge variants Mol wt (MW) AA sequence PTM Cell…

Poster Reprint ASMS 2023 Poster number MP 626 Multi-attribute monitoring of aggregates and charge variants of monoclonal antibody through native 2DSEC-MS-WCX-MS Sunil Kumar1, Tushar Sharad Savane1, Vadiraja Bhat2, Mike Knierman3, Anurag Singh Rathore1 1 Department of Chemical Engineering, Indian Institute…

Characterization and Monitoring of mAb Charge Variants via Online IEX-MS Samantha Ippoliti Waters MS User Meeting ASMS 2019 Atlanta, GA June 1, 2019 ©2019 Waters Corporation 1 Agenda 1. mAb CQA Characterization 2. Reverse Phase vs Ion Exchange Chromatography 3.…