Analysis of mRNA Cap Impurity Profiles and Capping Efficiency Using RapiZyme™ MC1 Ribonuclease

Application Note Oligo Mapping of mRNA Digests Using a Novel Informatics Workflow Catalin E. Doneanu, Chris Preston, Matt Gorton, Tatiana Johnson, Bala Addepalli, Ying Qing Yu Waters Corporation Abstract Recent advances in LC-MS and informatics technologies are supporting scientists in…

Application Note Head to Tail Analysis of mRNA by RapiZyme™ MC1 and Cusativin Alexandre F. Gomes*, Tatiana Johnston*, Balasubrahmanyam Addepalli * Equal contribution Waters Corporation, United States Published on August 10, 2026 Abstract Head to Tail Analysis of mRNA by…

Using Novel RNases to Measure 5’ Cap and Poly(A) Tail Modifications during Oligonucleotide Mapping LC-MS of messenger RNA Authors: Balasubrahmanyam Addepalli; Tatiana Johnston; Catalin Doneanu; Alexandre Gomes; Christian Reidy; Ying Qing Yu; Matthew Lauber Affiliations: WATERS CORPORATION, MILFORD MA 01757…

Application Note LC-MS Analysis of Two mRNA Critical Quality Attributes: 5’-Capping Efficiency and Average Poly(A) Tail Length and Heterogeneity Using the Xevo™ MRT Mass Spectrometer Catalin Doneanua, Alexandre F. Gomesa, Jo-Anne Rileyb, Ying Qing Yua a Waters Corporation, United States…