Antibody subunit analysis workflow on a quadrupole-Orbitrap mass spectrometer: from optimized sample preparation to data analysis

APPLICATION NOTE 73885 Complete characterization of monoclonal antibodies under native and denaturing conditions Kristina Srzentić1, Eugen Damoc2, Kai Scheffler3, Tom Buchanan4, Krisztina Radi4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Bremen, Germany 3 Germering, Germany 4 Hemel…

Application note | 001391 Biopharma Improved and universal inline electrochemical reduction and subunits LC-MS analysis of multiple classes of monoclonal antibodies Authors Application benefits Sara Carillo, Tomos E. Morgan, Hendrik1 1 • Reduced mAb and reduced subunits analysis can be…

RES MS analysis of antibodies at the protein and peptide levels is critical during development and production of biopharmaceuticals. The compositions of current generation therapeutic proteins are often complex due to their heterogeneity caused by various modifications which are relevant…

Technical note | 003318 Biopharma In-depth characterization of monoclonal antibodies Intact mass analysis and middle-down mass spectrometry approaches on an Orbitrap Ascend BioPharma Tribrid mass spectrometer Authors Goal Jingjing Huang, Christopher Mullen, To assess the performance of the Thermo Scientific™…