In-depth characterization of monoclonal antibodies

In-Depth Characterization of Monoclonal Antibodies Using Intact Mass Analysis and Middle-down Approaches on Orbitrap Ascend BioPharma Tribrid Mass Spectrometer Jingjing Huang, Christopher Mullen, Kristina Srzentić, Graeme McAlister, Vlad Zabrouskov, Rafael Melani Thermo Fisher Scientific, San Jose, California, USA Abstract Purpose:…

APPLICATION NOTE 73885 Complete characterization of monoclonal antibodies under native and denaturing conditions Kristina Srzentić1, Eugen Damoc2, Kai Scheffler3, Tom Buchanan4, Krisztina Radi4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Bremen, Germany 3 Germering, Germany 4 Hemel…

Complete characterization of KADCYLA using a new Orbitrap Excedion Pro BioPharma hybrid mass spectrometer with electron-transfer/higher-energy collision dissociation (EThcD) fragmentation Xiaoxi Zhang1, Roberto Gamez2, Reiko Kiyonami3, Cong Wang4, Peter Krueger4, Heiner Koch4, Min Du3 1 Thermo Fisher Scientific, Shanghai, China;…

APPLICATION NOTE 74160 Proton transfer charge reduction (PTCR) improves spectral matching and sequence coverage in middle-down analysis of monoclonal antibodies Luca Fornelli,1 Ryan N. Oates,1 Kristina Srzentić,2 Christopher Mullen,3 John E. P. Syka,3 and Romain Huguet3 University of Oklahoma, Norman,…