Characterization and Sequencing of Duplex siRNA Using the BioAccord™ LC-MS System and Vion IMS QTof Mass Spectrometer in Combination With the waters_connect™ CONFIRM Sequence App

Application Note Investigating the Strength of the Sense and Antisense Oligonucleotide Interaction to Form siRNA Duplexes Using the BioAccord™ LC-MS System Catalin Doneanu, Ying Qing Yu Waters Corporation, United States Published on August 03, 2026 Abstract A hydrophilic interaction liquid…

Application Note Expanding the Antibody-Oligo Conjugate (AOC) Characterization Toolbox: Part 2Denaturing and Non-denaturing Analysis of siRNA Payload Samantha Ippoliti, Ying Qing Yu, Connor Brandenburg, Tara MacCulloch Waters Corporation, United States Note: This is the second application note in a three-part…

Comprehensive LC-MS Analytical Assays for Antibody-Oligonucleotide Conjugate (AOC) and siRNA Linker-Payload Characterization Samantha Ippoliti1; Brad Williams1; Ying Qing Yu1; Connor Brandenburg; Tara MacCulloch; Michelle Chen2; Sophia Kenrick2 1 Waters Corporation, Milford, MA; 2Waters Corporation, Goleta, California INTRODUCTION METHODS Antibody-oligo conjugates…

Application Note A Xevo™ G3-based Workflow for Purity Determination, Intact Mass Measurement, and MS/MS Sequencing of Impurities Detected in Synthetic Oligonucleotides Catalin E. Doneanu, Jonathan Fox, Brad J. Williams, Chris Knowles, Ying Qing Yu Waters Corporation Abstract This study evaluates…