Enhancing Subunit-Level Profiling of mAbs and ADCs with MS- Quality Difluoroacetic Acid

HIGH SENSITIVITY LC-MS PROFILING OF ANTIBODY DRUG CONJUGATES WITH DIFLUOROACETIC ACID ION PAIRING AND A HIGHCOVERAGE PHENYL-BONDED STATIONARY PHASE Jennifer Nguyen1,2, Jacquelynn Smith3, Olga V. Friese3, Jason C. Rouse4, Daniel P. Walsh1, and Matthew A. Lauber1 1 Waters Corporation, Milford,…

[ APPLICATION NOTE ] A Novel Phenyl Bonded Phase for Improved Reversed-Phase Separations of Proteins Jennifer M. Nguyen, Lavelay Kizekai, Dan Walsh, Jim Cook, and Matthew A. Lauber Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Polyphenyl bonded phase…

[ APPLICATION NOTE ] Improving the Recovery of Intact Antibodies from Reversed-Phase Separations Using a BioResolve RP mAb Polyphenyl Column Ximo Zhang, Robert Birdsall, and Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS INTRODUCTION Method for improving the…

Using Hydrophilic Interaction Chromatography for Heightened Product Characterization to Overcome Challenges with Hydrophobic Monoclonal Antibodies and Antibody Drug Conjugates Jacquelynn Smith,1 Matthew A. Lauber,3 Stephan M. Koza,3 Erin E. Chambers,3 Jason C. Rouse,2 and Olga V. Friese1 Biotherapeutics Pharm. Sci.,…