Myth busting: “You cannot sequence oligonucleotides over 20 to 30 nucleotides long by LCMS/MS” Learn how to routinely sequence 100 nt oligonucleotides

Oligonucleotide sequencing by LC-MS/MS: A novel Approach for Characterization and Quality Control of mRNA-based Vaccines and Biotherapeutics Alexander Schwahn, Angela Criscuolo, and Ken Cook The world leader in serving science 1 [email protected] | ISC2022 Why have oligonucleotides become so popular?…

Customer application note | 000723 Biotechnology mRNA direct sequence mapping using automated partial digestion with magnetic nuclease and LC-HRMS Authors Application benefits Mark Dickman1, Christina Vanhinsbergh1, Jon Bardsley , Ken Cook , Andrew 2 2 Williamson2, Jennifer Sutton3, Keeley Murphy3…

High-throughput LC/MS characterization of mRNA therapeutics using a fast DDA method on the Orbitrap Astral MS Tabiwang Arrey1; Angela Criscuolo1; Matthew James Garland1, Bernd Hagedorn1, Keeley Murphy2; Eugen Damoc1, 1Thermo Fisher Scientific, Bremen, Germany; 2Thermo Fisher Scientific, San Jose, CA…

APPLICATION NOTE 73870 Identification and quantitation of oligonucleotides, impurities, and degradation products Haichuan Liu1, Kevin Guo2, Jennifer Sutton1, Keeley Murphy1, Min Du2 1 Thermo Fisher Scientific, San Jose, CA 2 Thermo Fisher Scientific, Boston, MA Keywords: Oligonucleotide, RNA, impurity, purification,…