Reverse phase analysis of free complex glycans

Dave Thomas and Ian Acworth Thermo Fisher Scientific, Chelmsford, MA Ap plica t ion Note 1 1 27 Label-Free Analysis by UHPLC with Charged Aerosol Detection of Glycans Separated by Charge, Size, and Isomeric Structure Key Words Glycan Quantitation, Glycan…

Udayanath Aich1, Julian Saba2, Xiaodong Liu1 and Chris Pohl1 1 Thermo Fisher Scientific, Sunnyvale, CA, USA; 2Thermo Fisher Scientific, Mississuaga, ON, Canada Appli cat i on N ote 2 0 8 2 7 Structural Analysis of Native N-Glycans Released from…

In M M G N-linked glycans are released from proteins by PNGase-F. The released glycans are separated by ultra high performance liquid chromatography (UHPLC) on a new UHPLC platform that integrates the charged aerosol detector into the system for increased…

Alexandra Manka and Mauro De Pra Thermo Fisher Scientific, Germering, Germany Key Words Biosimilars, Biotherapeutics, Bovine Fetuin, Fluorescence Detector, Glycan Analysis, IgG Goal To demonstrate successful separation and detection of 2-aminobenzamide (2-AB)-labeled complex glycan libraries on a Thermo Scientific™ Vanquish™…