Characterization of a synthetic double stranded siRNA using high-resolution mass spectrometry

Customer application note | 004514 Pharma Efficient early-phase screening of siRNA purity and impurities via denaturing IP-RPLC-UV with a dual-channel UHPLC system Authors Application benefits Martina Lioi, Selin Bagci, David Benda, • Increased efficiency and time savings: Parallel scouting across…

APPLICATION NOTE 73789 Oligonucleotide mapping using BioPharma Finder software Authors: Haichuan Liu1, Kevin Guo2, Jennifer Sutton1, Min Du2 Thermo Fisher Scientific, San Jose, CA Thermo Fisher Scientific, Boston, MA 1 2 Keywords: Oligonucleotide, DNA, RNA, data dependent acquisition, tandem mass…

0 Introduction 100 80 Relative Abundance Synthetic ONs with different functionalities including antisense ONs, small interfering RNAs (siRNAs), aptamers and immunostimulatory RNAs (isRNAs) are candidate therapeutic agents due to their specificity, and well-established synthesis and modification technologies. Still characterization is…

Application Note Biopharma Complementary LC/MS and UV‑Vis Spectrophotometry for siRNA Quality Control and Thermal Stability Assessment Authors Abstract Yulan Bian and Ravindra Gudihal Agilent Technologies, Inc. Small interfering RNAs (siRNAs) are increasingly important therapeutic agents, which require robust analytical strategies…