Raw Material Testing: Developing Methods for Quality Control of Phosphoramidites used in the Chemical Synthesis of Oligonucleotides

Application Note Biopharma/Pharma Analyzing Raw Material for Oligonucleotide Synthesis Flexible and robust method development for the analysis of nucleoside phosphoramidites with the Agilent 1290 Infinity II Bio LC System 100 Response (mAU) 80 60 40 20 0 0 5 10…

Application note | 001949 Biotech LC/UV/HRAM MS-based impurity profiling and structure elucidation of phosphoramidite raw materials used for oligonucleotide synthesis Application benefits Authors Sven Hackbusch , Gary Held , Yi Zhang , 1 Min Du 2 3 • Sensitive detection…

Application Note Biopharma and Pharma Identification of Commercially Available Oligonucleotide Starting Materials Directly Through Containers Authors Frederic Pulliere and Christopher Welsby Agilent Technologies, Inc. Abstract Handheld Raman spectrometers can selectively identify oligonucleotide starting materials, also known as phosphoramidites, directly through…

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…