Exploring Depth and Breadth of a Protein Complex Mixture with Top-Down Data-Independent Acquisition Using an Orbitrap Fusion Tribrid Mass Spectrometer

Technical note | 003155 Clinical Enhancing detection of hemoglobin variants in clinical research using dried blood spot and high-resolution accurate mass (HRAM) Orbitrap mass spectrometry Authors Application benefits Yvonne E. Song, Jingshu Guo, • Direct protein extraction from dried blood…

proteins on a proteomics scale is challenging and requires significant method optimization of front–end separation, instrument parameters and data analysis. In this study, we developed a general RP-LC-MS method for intact/ top-down analysis on an Orbitrap Fusion mass spectrometer using…

Technical note | 003497 Structural biology Decipher intricate glycoproteins using data-independent acquisition-proton transfer charge reduction and native top-down mass spectrometry Authors Introduction Weijing Liu, Yuqi Shi, Christopher Mullen, The SARS-CoV-2 pandemic underscores the urgent need for rapid viral glycoprotein Julian…

Low Attomole Limit of Quantification on an Orbitrap Fusion Introduction Lumos Tribrid Mass Spectrometer One of the major challenges in Proteomics is the quantification of low abundance proteins and peptides of biological relevance such as transcription factors or low stoichiometry…