UHPLC Analysis of 2-Aminobenzamide- Labeled Glycans with the Vanquish Flex System

Alexandra Manka and Mauro De Pra Thermo Fisher Scientific, Germering, Germany Key Words Biosimilars, Biotherapeutics, Bovine Fetuin, Fluorescence Detector, Glycan Analysis, IgG Goal To demonstrate successful separation and detection of 2-aminobenzamide (2-AB)-labeled complex glycan libraries on a Thermo Scientific™ Vanquish™…

APPLICATION NOTE 21764 Accurate and precise quantification of mAb-released N-glycans with an amide HILIC column Authors Xin Zhang, Xuefei Sun Thermo Fisher Scientific, Sunnyvale, CA, USA Application benefits • Proven intra- and inter-column reproducibility in retention time, backpressure, and…

O-linked glycans are released from proteins by reductive β-elimination. The released glycans are separated by ultra high performance liquid chromatography (UHPLC) on a new UHPLC platform that integrates the charged aerosol detector into the system for increased performance and ease…

Dave Thomas and Ian Acworth Thermo Fisher Scientific, Chelmsford, MA Ap plica t ion Note 1 1 27 Label-Free Analysis by UHPLC with Charged Aerosol Detection of Glycans Separated by Charge, Size, and Isomeric Structure Key Words Glycan Quantitation, Glycan…