New prm-PASEF for highly multiplexed targeted acquisition in clinical samples

prm-PASEF®: enabling high-throughput, high sensitivity targeted proteomics The prm-PASEF acquisition method has been developed to translate the advantages of the parallel accumulation serial fragmentation (PASEF) acquisition strategy to the targeted proteomics field. Abstract In comparison with standard selected and parallel…

High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and…

timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics The timsTOF Pro powered by PASEF enables sequencing speed of > 120 Hz, high sensitivity and robustness as well as an additional dimension of…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…