Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF): Bottom-up proteomics with near optimal ion usage

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…

Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF) The timsTOF Pro with diaPASEF enables deeper proteome coverage in a single 4D shotgun proteomics experiment, with highly reproducible qualitative and quantitative results – making it a near-ideal mass analyzer…

POSTER NOTE 65541 Improved Identification, Improved Identification, Quantification Accuracy, and Workflow Q Efficiency using a Modified Quadrupole Orbitrap Mass and Ta Mass Spectrometer Aaron M. Robitaille N. Arrey 2, Markus Kellma Spectrometer and Tandem Mass Tags1, Tabiwang (TMT) Approach 1…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…