Optimizing HPLC Separations: Samples with Widely Differing Polarities

A P P L I C AT I O N S N O T E B O O K www.waters.com Introduction Most chromatographers have experienced problems retaining and separating polar compounds using conventional reversed-phase (RP) chromatography. These difficult-to-analyze compounds either…

VOL 18.1 TheReporter www.sigma-aldrich.com/TheReporter New Paradigm for HPLC Method Development Column: Mobile Phase: Discovery, 5cm x 4.6mm ID, 5µm particles A: O.1% HCO2H (pH 2.5) B: MeCN 0 - 4% in 0.8 min., 4 - 30% in 1.7 min. 3mL/min…

VOL 18.6 TheReporter www.sigma-aldrich.com/TheReporter (continued on page 3) LC issue Conditions for A, B & C differ only in column length, flow and gradient. Panel A. Column: Discovery C18, 15cm x 4.6mm ID, 5µ Cat. No.: 504955 Sample: Gradient RP…

APPLICATION NOTE 72877 Accelerated method development for the separation of water-soluble vitamins by RP-HPLC with UV detection using an empirical approach to predict separation Authors Sylvia Grosse, Soo Hyun Park, Mauro De Pra, Frank Steiner Thermo Fisher Scientific, Germering, Germany…