Fast Gradients for RP-HPLC

VOL 18.1 TheReporter www.sigma-aldrich.com/TheReporter New Paradigm for HPLC Method Development Column: Mobile Phase: Discovery, 5cm x 4.6mm ID, 5µm particles A: O.1% HCO2H (pH 2.5) B: MeCN 0 - 4% in 0.8 min., 4 - 30% in 1.7 min. 3mL/min…

VOL 18.3 TheReporter www.sigma-aldrich.com/TheReporter Optimizing HPLC Separations: Samples with Widely Differing Polarities When confronted with a sample containing widely varying polarities, the LC chromatographer will typically rely on gradient elution. However, if gradient elution is not an option, isocratic elution…

VOL 18.10 TheReporter www.sigma-aldrich.com/TheReporter Scale-Up of Fast Analytical to Fast Preparative Separations This article describes a simple, reproducible, and predictable process for scaling up from fast analytical to fast preparative HPLC separations. Chromatographers performing lab scale preparative or larger process…

VOL 20.3 TheReporter sigma-aldrich.com/TheReporter The Effect of Ion Pair Reagent on Reversed-Phase Selectivity of Polypeptide Separations Reversed-phase high performance liquid chromatography (RP-HPLC) has been a preferred method for resolution of peptides by virtue of its resolving power and suitability of…