Utilization of the ACQUITY PREMIER System and Column for Improved Oligonucleotide Bioanalytical Chromatographic Performance

Application Note Improved Oligonucleotide SPE-LC-MS Analysis Using MaxPeak High Performance Technology Kathryn Brennan, Mary Trudeau Lame, Michael Donegan, Paul D. Rainville Waters Corporation Abstract The demand for highly selective and sensitive LC-MS bioanalytical assays in support of research and development…

Application Note Sensitive LC-MS/MS Bioanalytical Quantitation of Antisense Oligonucleotides Suma Veeramachineni, Mark D. Wrona Waters Corporation Abstract This application demonstrates the quantitative and qualitative capabilities of the Waters™ Xevo™ TQ Absolute Tandem Quadrupole Mass Spectrometer (MS/MS) and its suitability for…

Improving LC-MS/MS Bioanalytical Quantitation of Oligonucleotides using New High Performance Tandem Quadrupole Mass Spectrometer Suma Veeramachineni1; Suraj Dhungana1; Ying-Qing Yu1; Mark Wrona1; 1Waters Corporation, Milford, MA Introduction Results Improving LC-MS/MS bioanalytical performance especially for ESI negative analytes has been of…

Utilization of Novel Low Adsorption Chromatographic Surface Technology for Improved Bioanalytical Quantitation of Oligonucleotides Mary Trudeau & Kathryn Brennan, Waters Corporation, MA , USA Introduction LLE Extraction Add 100 µL 25:24:1 Phenol:Chloroform:Isoamyl Alcohol Vortex ~ 30 minute Centrifuge @ 14,000…