It’s Not All About the Column: The Role of the Mobile Phase and Your Instrument

RECORD | Already taken place Mo, 14.6.2021
In this presentation, we’ll look at how our mobile phase components help us get the best separation possible. We will look more closely at what makes up our aqueous component as well as our choice of organic.
Agilent Technologies: It’s Not All About the Column: The Role of the Mobile Phase and Your Instrument
Agilent Technologies: It’s Not All About the Column: The Role of the Mobile Phase and Your Instrument

Choosing the right column is a critical part of successful chromatography but that’s not all it takes.

We’ll discuss:

Choosing a buffer
  • Options
  • pH ranges
  • Volatile vs. non-volatile
Organic
  • Choices
  • Compatibility with aqueous component and sample

We’ll also talk about how our instrument can contribute to our success or defeat our efforts without us knowing why.

Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.

Agilent Technologies
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